A Lateral Flow Device for Point-of-Care Detection of Doxorubicin

Abstract

A simple, rapid, and sensitive point-of-care (POC) device for the on-site detection of doxorubicin was developed. The proposed method relies on the naked-eye detection of the intrinsic fluorescence of the drug in a lateral flow device (LFD) configuration, exploiting the biological recognition of DNA probes and avoiding the use of expensive antibodies and sophisticated instrumentations. The POC assay does not require any pre-treatment or purification step and provides an immediate visual readout, achieving a limit of detection as low as ca. 1 ng doxorubicin, outperforming several laboratory-based instrumental techniques. The POC method was proven useful for the detection of trace amounts of the drug both in the case of water solutions (to simulate surface contaminations) and in urine samples, opening promising perspectives for routine monitoring of doxorubicin, with potential benefit to healthcare workers and personalized chemotherapies.

Bottom‐up DNA nanostructure‐based paper as point‐of‐care diagnostic: From method to device

“…Reproduced with permissions. 47,48 Copyright 2021, MDPI and Copyright 2022, MDPI. (B) Using ssDNA probe for the detection of multiple SARS-CoV-2 genes by LFA.…”

“… 47 Furthermore, Pomili et al. used this recognition model to achieve a limit of detection (LOD) of doxorubicin as low as 1 ng by using a dsDNA designed to recognize and capture doxorubicin in 1D paper‐based assays 48 …”

“…Using dsDNA probe recognize and capture doxorubicin. Reproduced with permissions 47,48 . Copyright 2021, MDPI and Copyright 2022, MDPI.…”

A multi-line platinum nanozyme-based lateral flow device for the colorimetric evaluation of total antioxidant capacity in different matrices

“…3a). Moreover, exploiting smartphone pictures of these samples, we performed a quantitative analysis of the detection zones, calculating the red (R) value (complementary color of blue) 52,53 for each test line (Fig. 3b).…”

Colorimetric Characterization of Color Imaging System Based on Kernel Partial Least Squares

“…Colorimetric characterization is very important for image high-fidelity display, colorimetric measurement, color reproduction, color analysis, color management of different devices [ 5 ] and color appearance prediction [ 6 ], and the requirements for the accuracy and stability of color characterization are increasing for different applications. Color analysis [ 7 , 8 , 9 , 10 ] plays a very important role in the evaluation of the performance of many technologies including paper-based devices that use colorimetric reactions and that researchers have used with respect to different color spaces such as weighted RGB, greyscale, etc.…”