Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of H7N9 Influenza Viruses

Sun, Zhihao; Shi, Baolan; Meng, Fanqiang; Ma, Ruonan; Hu, Qiongyi; Qin, Tao; Chen, Sujuan; Peng, Daxin; Liu, Xiufan

doi:10.3389/fmicb.2018.02069

Cited by 28 publications

(23 citation statements)

References 27 publications

(28 reference statements)

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“…The detection limit for swab samples and tissue homogenates was 4.5 log10EID 50 , and the sensitivity was lower than our strips (2.4 log10EID 50 ). Liu X [18] developed a colloidal gold-based immunochromatographic strip for rapid detection of H7N9 in uenza viruses with a limit of detection of 2.5 log10EID 50 , less sensitive than our strips (2.4 log10EID 50 ). Kang [20] developed a rapid immunochromatographic test for hemagglutinin antigen of H7 subtype in patients infected with novel avian in uenza A (H7N9) virus with a limit of detection of 10 3 TCID 50 , less sensitive than our strips (10 2.6 TCID 50 ).…”

Section: Discussionmentioning

confidence: 99%

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Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

Wang

et al. 2020

Preprint

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Background: H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have close contacted with poultry in the live poultry market. Therefore, it is necessary to develop a rapid point-of-care testing (POCT) technique for H7N9 AIV detection.Methods: The H7N9 AIV was inactivated and purified, and was used as the antigen to immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced through the hybridoma technique. The McAb 10A8 was conjugated with colloid gold as detecting antibody; McAb 9B6 was dispensed on the nitrocellulose membran as the capture test line and the Goat-anti mouse IgG antibody was dispensed as control line respectively. The immunochromatographic strip was prepared.Results: The analysis of ELISA and virus neutralization test showed that the obtained McAbs specifically recognized H7 HA. Based on the prepared strip, the detection of H7 AIV was achieved within 10 min. No cross-reaction occurred between H7 AIVs and other tested viruses. The detection limit of the strip for H7 was 2.4 log10EID50/0.1 mL for chicken swab samples.Conclusion: The McAbs were specific for H7 and the immunochromatographic strip developed in this study was convenient, rapid and reliable for the detection of H7 AIV. The strip could provide an effective method for the rapid and early detection of H7 AIV.

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Section: Discussionmentioning

confidence: 99%

“…Two H7-HA McAbs were developed, and identi ed by HI assay and VN assay. The HI and VN titers of different McAbs [18] were different, which may lead to differences in the speci city and a nity of McAbs. At the same time, different batches of ascites may affect the performance of the test strips.…”

Section: Discussionmentioning

confidence: 99%

Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

Wang

et al. 2020

Preprint

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“…Our assay could be applied as the standardized H7 quantification method. While several rapid H7 antigen diagnosis strips have been developed, the advantages of enhanced sensitivity and suitable for large‐scale samples make our ELISA deserve to become a valuable tool in resource‐limited hospitals or laboratories. In addition, the combination of current H7 with our previous developed N9 immunoassays will greatly aid the simultaneous identification of H7N9 virus in clinic .…”

Section: Discussionmentioning

confidence: 99%

“…Our assay could be applied as the standardized H7 quantification method. While several rapid H7 antigen diagnosis strips have been developed,16,17 the advantages of enhanced sensitivity and suitable for large-scale…”

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confidence: 99%

Establishment of sandwich ELISA for detecting the H7 subtype influenza A virus

Ruan

Sun

Chen

et al. 2019

Journal of Medical Virology

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Avian H7N9 subtype influenza virus infects human with high case‐fatality rate since it emerged in 2013. Although the vaccination has been rapidly used in poultry due to the emergence of highly pathogenic strain, this virus remains prevalent in this region. Thus, rapid diagnosis both in poultry and human clinic is critically important for the control and prevention of H7N9 infection. In this study, a batch of H7 subtype‐specific monoclonal antibodies (mAbs) were developed and a pair of mAb, 2B6, and 5E9 were used to establish a double‐antibody sandwich enzyme‐linked immunosorbent assay (ELISA) to quantify H7 protein and detect influenza A virus baring H7 subtype HA. The lowest detection limit for the recombinant H7 protein was 10 ng/mL and 0.5 HAU/50 μL of A/Guangdong/17SF003/2016(H7N9), 2 HAU/50 μL of A/Netherlands/219/2003(H7N7) and A/Anhui/1/2013(H7N9) for live virus, respectively. The ELISA could not only detect the prevailing H7N9 virus, but also antigenic drift H7 subtype viruses, showing excellent sensitivity and high specificity. Hence, it could serve as a valuable approach to diagnose H7 subtype virus which showed great potential to cause pandemic, as well as antigen quantification.

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“…The analysis can be completed in 20 min without sophistic equipment. The method has been widely used to detect multiple antigens for instance hormones, antibodies in serum ( Zhao et al., 2017 ), viruses ( Sun et al., 2018 ), bacteria ( Wen-de et al., 2017 ), parasites ( Zhuo et al., 2017 ), biotin ( Yao et al., 2017 ), microelement ( Fu et al., 2017 ), and drug residue ( Sakamoto et al., 2017 ). In this study, a CGIS based on monoclonal antibodies against R. anatipestifer outer membrane protein A ( OmpA ) was developed to rapidly detect R. anatipestifer in ducks.…”

Section: Introductionmentioning

confidence: 99%

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

et al. 2020

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Riemerella anatipestifer is one of the major bacterial pathogens of ducks and causes significant economic losses in poultry agriculture. Usually, methods for detecting R. anatipestifer infection need specialized equipment and highly skilled personnel. In this study, a novel colloidal gold immunochromatographic strip was developed for rapid detection of R. anatipestifer in ducks. The monoclonal antibodies 2D5 and 2A6 against R. anatipestifer were used as colloidal gold-labeled protein and capture protein, respectively, to recognize the bacteria in tryptic soy broth medium culture and in hearts of infected ducks. The goat anti-mouse IgG antibody was labeled on nitrocellulose membrane as a control for C line. The labeling pH was optimized as 10.0, and the concentration of 2D5 labeled to colloidal gold particles was optimized as 18 μg/mL. The strip specifically detected serotypes 1, 2, and 10 R. anatipestifer strains and showed no cross-reaction with Escherichia coli , Salmonella enterica, and Pasteurella multocida strains. The sensitivity of the strip for detecting R. anatipestifer was 1.0 × 10 6 colony forming unit. The strips remained stable for up to 8 mo at 4°C, and the detection can be completed within 15 min. The strip can detect R. anatipestifer in hearts of the ducks experimentally infected with R. anatipestifer but not infected with E. coli , which were also confirmed with bacterial isolation followed by multiplex polymerase chain reaction. These results suggested that the strips are reliable methods for identification of R. anatipestifer in laboratories and in duck farms.

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Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of H7N9 Influenza Viruses

Cited by 28 publications

References 27 publications

Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

Establishment of sandwich ELISA for detecting the H7 subtype influenza A virus

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

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