Development of a chitosan hydrogel containing flavonoids extracted from Passiflora edulis leaves and the evaluation of its antioxidant and wound healing properties for the treatment of skin lesions in diabetic mice

Abstract

This study aimed to develop a chitosan-based hydrogel containing a mixture of flavonoids isolated from the leaves of Passiflora edulis Sims and to evaluate its stability, antioxidant properties, and wound healing effects on cutaneous lesions in diabetic rats. in vitro studies were carried out to evaluate the biocompatibility and flavonoid release from the chitosan hydrogel. in vivo wound healing studies were conducted on male Wistar rats, where the injured tissue was removed for histological analysis and determination of lipid peroxidation, superoxide dismutase activity, and glutathione peroxidase activity. From the histological analysis and macroscopic evaluation of the contraction of the wounds, it was observed that the formulation presented wound healing properties. In addition, treatment of the wound with the formulation stimulated the antioxidant defense system, suggesting a beneficial effect during the treatment of skin lesions in diabetic rats, especially in the first few days after wounding. According to these results, we can conclude that the chitosan hydrogel containing the flavonoid analyzed in this study has potential use as dressings in the treatment of wounds. K E Y W O R D S antioxidant, chitosan, flavonoid, hydrogel, Passiflora edulis, wound healing

Passiflora edulis: An Insight Into Current Researches on Phytochemistry and Pharmacology

“…Peel flour of P. edulis intake increased glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1, improved the insulin sensitivity in high-fat diet-induced obesity rats by increasing the glucose disappearance rate (Lima et al, 2016), and also prevents insulin resistance induced by low-fructose-diet in rats. In addition, the leaf extract of P. edulis full of flavonoids also has a health benefit to the diabetic state, and show the prevent effect on the appearance of its complications (Salles et al, 2020;Soares et al, 2020).…”

Hydrogels for the Delivery of Plant-Derived (Poly)Phenols

“…HGs to vehicle RU [ 24 , 25 ], CGA [ 26 ], Q [ 27 ], FA [ 28 ], and oligomeric proanthocyanidins (OPC) [ 29 ], as well as polyphenol-rich plant extracts [ 30 , 31 ], appeared effective in ameliorating wound healing in in vivo rat and mouse models, and also in animals with diabetes mellitus experimentally induced by streptozotocin or alloxan ( Table 1 ) [ 28 , 29 , 30 , 31 ]. An innovative RU-conjugated CS-HG was projected by Tran N.Q.…”

“…A CS-based HG containing a mixture of flavonoids extracted from Passiflora edulis Sims leaves was prepared by Soares’s research group ( Figure 7 ) [ 31 ]. The HG was obtained by simple addition of glycerin to an acetic acid solution of the polymer, together with small amounts of methylparaben and propylparaben as preservatives.…”

Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model

“…Based on a previous report, in every 30 s, one leg is amputated as a result of impaired wound healing in diabetic patients worldwide [ 2 ]. Owing to the absence of measures for prevention, non-healing and poorly healing wounds pose a serious global health threat with elevated DM prevalence [ 3 ]. The critical cause of the poor healing of the diabetic wound is prolonged inflammation, poor vascularization and epithelization, and deficient collagen deposition [ 4 ].…”

“…The critical cause of the poor healing of the diabetic wound is prolonged inflammation, poor vascularization and epithelization, and deficient collagen deposition [ 4 ]. Additionally, the sustained reactive oxygen species (ROS) production would further delay the recovery of wounds in diabetic patients [ 3 , 5 ]. Thus, the wound dressing biomaterials with the ability to promote diabetic wound healing have been widely studied.…”