Development of a broad-spectrum Salmonella phage cocktail containing Viunalike and Jerseylike viruses isolated from Thailand

Phothaworn, Preeda; Supokaivanich, Rattaya; Lim, Jiali; Klumpp, Jochen; Imam, Mohammed; Kutter, Elizabeth; Galyov, Edouard E.; Dunne, Matthew; Korbsrisate, Sunee

doi:10.1016/j.fm.2020.103586

Cited by 32 publications

(21 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Remarkably, SPHG1 and SPHG3 did not encode any integrases, virulence associated-factors, or antimicrobial-resistance genes. For biocontrol applications in food, strictly lytic phages are used to avoid potential threats (e.g., the transduction of virulence factors) that are associated with the lysogenic (temperate) phages [45,77,78]. Genome analysis identified SPHG1 and SPHG3 as virulent phages in the Myoviridae and Ackermannvirindae families, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…To be applied as biocontrol agents in the food industry, phages should have certain features, as a broad host range, persist the food processing environment, and do not have any pathogenic or allergic-associated properties [45,46]. Moreover, temperate, or lysogenic phages are disqualified as biocontrol agents, because they are less effective and can integrate into their hosts, which facilitates the transferring of antibiotic-resistant genes or virulence genes to their bacterial host that increase the potential of generating pathogenic strains [27,47].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

et al. 2021

View full text Add to dashboard Cite

Foodborne salmonellosis is a global threat to public health. In the current study, we describe the isolation and characterization of two broad-spectrum, lytic Salmonella phages: SPHG1 and SPHG3 infecting a multidrug-resistant Salmonella Typhimurium EG.SmT3. Electron microscopy and whole genome analysis identified SPHG1 as a Myovirus, while SPHG3 as a new member of the genus “Kuttervirus” within the family Ackermannviridae. SPHG1 and SPHG3 had a lysis time of 60 min. with burst sizes of 104 and 138 PFU/cell, respectively. The two phages were robust at variable temperatures and pH ranges that match the corresponding values of most of the food storage and processing conditions. A phage cocktail containing the two phages was stable in the tested food articles for up to 48 h. The application of the phage cocktail at MOIs of 1000 or 100 resulted in a significant reduction in the viable count of S. Typhimurium by 4.2 log10/sample in milk, water, and on chicken breast. Additionally, the phage cocktail showed a prospective ability to eradicate and reduce the biofilm that formed by S. Typhimurium EG.SmT3. A phage cocktail of SPHG1 and SPHG3 is considered as a promising candidate as a biocontrol agent against foodborne salmonellosis due to its broad host ranges, highly lytic activities, and the absence of any virulence or lysogeny-related genes in their genomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

et al. 2021

View full text Add to dashboard Cite

show abstract

“…It has also been proposed as a therapeutic or decontaminating agent ( Whichard et al, 2003 ; Radford et al, 2017 ). Since then, many studies have reported the isolation and successful application of phages for Salmonella control ( Tang et al, 2019 ; Abhisingha et al, 2020 ; Li et al, 2020 ; Phothaworn et al, 2020 ). Salmonella serotyping is important for disease control and prevention.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

et al. 2021

View full text Add to dashboard Cite

Salmonella is a widely distributed foodborne pathogen. The use of Salmonella phages as biocontrol agents has recently gained significant interest. Because the Salmonella genus has high diversity, efforts are necessary to identify lytic Salmonella phages focusing on different serovars. Here, five Salmonella phages were isolated from soil samples, and vB_SalP_TR2 was selected as a novel phage with high lytic potential against the host Salmonella serovar Albany, as well as other tested serovars, including Corvallis, Newport, Kottbus, and Istanbul. Morphological analyses demonstrated that phage vB_SalP_TR2 belongs to the Podoviridae family, with an icosahedral head (62 ± 0.5 nm in diameter and 60 ± 1 nm in length) and a short tail (35 ± 1 nm in length). The latent period and burst size of phage vB_SalP_TR2 was 15 min and 211 PFU/cell, respectively. It contained a linear dsDNA of 71,453 bp, and G + C content was 40.64%. Among 96 putative open reading frames detected, only 35 gene products were found in database searches, with no virulence or antibiotic resistance genes being identified. As a biological control agent, phage vB_SalP_TR2 exhibited a high temperature and pH tolerance. In vitro, it lysed most S. Albany after 24 h at 37°C with multiplicities of infection of 0.0001, 0.001, 0.01, 0.1, 1, 10, and 100. In food matrices (milk and chicken meat), treatment with phage vB_SalP_TR2 also reduced the number of S. Albany compared with that in controls. These findings highlighted phage vB_SalP_TR2 as a potential antibacterial agent for the control of Salmonella in food samples.

show abstract

“…In the advent, probiotics fail to significantly reduce AR Salmonella; combinatorial approaches involving vaccines, synbiotics, and phage treatments are actively being explored [104,105] as long-term interventions in production agriculture. The success of these approaches requires collaborations between the FDA and WHO to safely identify, scale-up, and apply new interventions as soon as possible, particularly as the global food industry enters the post-antibiotic era.…”

Section: Discussionmentioning

confidence: 99%

Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update

Hulme

2021

IJMS

View full text Add to dashboard Cite

Antibiotic and multi-drug resistant (MDR) Salmonella poses a significant threat to public health due to its ability to colonize animals (cold and warm-blooded) and contaminate freshwater supplies. Monitoring antibiotic resistant Salmonella is traditionally costly, involving the application of phenotypic and genotypic tests over several days. However, with the introduction of cheaper semi-automated devices in the last decade, strain detection and identification times have significantly fallen. This, in turn, has led to efficiently regulated food production systems and further reductions in food safety hazards. This review highlights current and emerging technologies used in the detection of antibiotic resistant and MDR Salmonella.

show abstract

Development of a broad-spectrum Salmonella phage cocktail containing Viunalike and Jerseylike viruses isolated from Thailand

Cited by 32 publications

References 51 publications

Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update

Contact Info

Product

Resources

About