“…Matrix effects have been studied predominantly in environmental microbiology, microbial food safety and forensic analyses, where the amounts of target nucleic acids are often extremely small and the matrices are particularly diverse and challenging [ 28 , 29 , 30 ]. In contrast, with the exception of forensically and microbiologically relevant body fluids and secretions, as well as food safety-relevant matrices such as muscle tissue and milk, the effects of mammalian matrices on qPCR results have been assessed and discussed only in a very limited manner and only for a limited number of tissue types [ 14 , 16 , 31 , 32 , 33 , 34 ].…”