Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5

Torisky, Rebecca S.; Kovács, László; Avdiushko, Sergei A.; Newman, Jeffrey D.; Hunt, Arthur G.; Collins, G. B.

doi:10.1007/s002990050360

Cited by 50 publications

(27 citation statements)

References 22 publications

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“…These include modifying the virulence of Agrobacterium tumefaciens strains (Hood et al 1993;Torisky et al 1997), sonication of explant tissues to increase the number of infection sites (Santarem et al 1998;Trick and Finer 1998), and addition of thiol compounds to the cocultivation medium (Ohhoft and Somers 2001;. In a comparative study to evaluate virulence of different strains of A. tumefaciens on soybean explants, strain KYRT1 was reported to be more virulent than other commonly used strains, including EHA105, GV3850 and LBA4404 (Torisky et al 1997;Meurer et al 1998). However, no transgenic plants have been recovered or reported via transformation with strain KYRT1.…”

Section: Introductionmentioning

confidence: 97%

Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant

Lee

Krasnyanski

et al. 2003

Theor Appl Genet

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An efficient transformation system was developed for multiple soybean [Glycine max (L.) Merrill.] cultivars using Agrobacterium-mediated gene transfer. A significantly high number of hygromycin-resistant somatic embryos (SEs) was obtained when immature zygotic cotyledons were inoculated with Agrobacterium tumefaciens strain KYRT1 and when the abaxial side of explants was oriented upwards (i.e., the adaxial side of explants was in contact with the medium). Most hygromycinresistant SEs on selective medium were induced along the periphery of the abaxial side of cotyledonary explants. Extended periods of selection (up to 10 weeks postcocultivation) increased the frequency of somatic embryogenesis, and more than 50% of selected SEs tested positive for b-glucuronidase (GUS). Following maturation and regeneration of selected SEs, ten independent transgenic soybean plants of cv Jack were obtained, and the overall transformation frequency ranged from 1.1 to 1.7%. Six and two transgenic plantlets were obtained from cvs Dwight and Williams, respectively. In addition, transgenic suspension lines were established from cvs Jack, Williams, Dwight, Rend and Ina. Molecular analysis of embryogenic lines and/or transgenic plants, established from different cultivars, confirmed stable integration, expression, and/or inheritance of transgenes in both T 0 and T 1 plants.

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Section: Introductionmentioning

confidence: 97%

Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant

Lee

Krasnyanski

et al. 2003

Theor Appl Genet

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“…Because the PCR product is inserted in the antisense orientation between the two restriction sites, the GUS transcript forms an inverted repeat structure with an 400-nucleotide spacer (pBI121-IR-GUS). The recombinant plasmid was then introduced by triparental mating into A. tumefaciens strain KYRT1 (Torisky et al, 1997), which can efficiently infect G. max plants. The strain was kindly provided by G.B.…”

Section: A Tumefaciens-mediated Systemic Gene Silencing Of Gusmentioning

confidence: 99%

Patterning of Virus-Infected Glycine max Seed Coat Is Associated with Suppression of Endogenous Silencing of Chalcone Synthase Genes

et al. 2004

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Most commercial Glycine max (soybean) varieties have yellow seeds because of loss of pigmentation in the seed coat. It has been suggested that inhibition of seed coat pigmentation in yellow G. max may be controlled by homology-dependent silencing of chalcone synthase (CHS) genes. Our analysis of CHS mRNA and short-interfering RNAs provide clear evidence that the inhibition of seed coat pigmentation in yellow G. max results from posttranscriptional rather than transcriptional silencing of the CHS genes. Furthermore, we show that mottling symptoms present on the seed coat of G. max plants infected with some viruses can be caused by suppression of CHS posttranscriptional gene silencing (PTGS) by a viral silencing suppressor protein. These results demonstrate that naturally occurring PTGS plays a key role in expression of a distinctive phenotype in plants and present a simple clear example of the elucidation of the molecular mechanism for viral symptom induction.

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“…The low efficiency of T-DNA transfer itself and the regeneration systems used to recover transformed plants are the two main limiting factors. The first was improved through the use of more virulent Agrobacterium strains (Torisky et al 1997) and addition of e.g. L-cysteine, dithiothreitol (DTT) and sodium thiosulfate during co-cultivation of explants with the bacteria Olhoft et al , 2003.…”

Section: Soybeanmentioning

confidence: 99%

Gene Transfer in Legumes

Atif¹,

Patat-Ochatt²,

Švábová

et al. 2012

Progress in Botany

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Development of a binary vector system for plant transformation based on the supervirulent Agrobacterium tumefaciens strain Chry5

Cited by 50 publications

References 22 publications

Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant

Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant

Patterning of Virus-Infected Glycine max Seed Coat Is Associated with Suppression of Endogenous Silencing of Chalcone Synthase Genes

Gene Transfer in Legumes

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