Development of 16 microsatellite markers for the Korean endemic Vicia hirticalycina (Fabaceae)

Abstract

Premise of the Study Vicia hirticalycina (Fabaceae) is a narrowly endemic species restricted to mountain valleys of southern Korea. To investigate its fine‐scale genetic diversity and differentiation in Korea, we developed polymorphic microsatellite markers.Methods and ResultsSixteen polymorphic microsatellite markers were developed from Illumina MiSeq data. In 74 individual plants from four populations, one to seven alleles were expressed for each locus. The levels of observed and expected heterozygosity ranged from 0.000 to 0.778 and from 0.000 to 0.738, respectively. Cross‐amplification was conducted with three related species and seven to 11 markers were successfully amplified.ConclusionsThese new microsatellite markers will be useful in future studies on the population genetics of V. hirticalycina.