Development of 1,25-dihydroxycholecalciferol receptor in the duodenal cytosol of chick embryo.

Oku, Tsuneyuki; Shimura, Fumio; Moriuchi, Sachiko; Hosoya, Norimasa

doi:10.1507/endocrj1954.23.375

Cited by 14 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cause for the incomplete response to the sterol in undifferentiated cells is unclear at present. Induction of calcium absorption on day 15 coincides with the appearance of the specific cytoplasmic receptor protein (Oku, Shimura, Moriuchi and Hosoya 1976), which is responsible for the transfer of the sterol to the nucleus for gene activation (Brumbaugh and Haussler 1974;. Thus, hormonal action of vitamin D is possible in principle even in undifferentiated absorptive cells.…”

Section: Expression Of Vitamin D Effects During Embryonic Developmentmentioning

confidence: 99%

Differential Response of Enterocytes to Vitamin D during Embryonic Development: Induction of Intestinal Inorganic Phosphate, D-Glucose and Calcium Uptake

Cross¹,

Peterlik²

1982

Horm Metab Res

View full text Add to dashboard Cite

Chick intestine maintained in organ culture at day 20 of embryonic life responds to appropriate dose levels of 1,25-dihydroxyvitamin D3 or vitamin D3 in the culture medium by stimulation of Na+-dependent uptake of inorganic phosphate (Pj) and D-glucose as well as by increased calcium absorption. The intensity of each single response to vitamin D varies along the small intestine: Only the jejunum shows high vitamin D increments of all three transport systems under investigation. Inhibition of vitamin D responses by actinomycin D suggests the involvement of gene activation in the sterol's action of raising intestinal transport activities. Determination of the developmental pattern of each vitamin D-dependent transport system showed that only calcium absorption could be induced in undifferentiated cells on day 15, while complete expression of vitamin D action, including elevation of Na+-dependent uptake of D-glucose and Pj, required a fairly advanced state of epithelial maturation and was thus not observed before the final day 20 of embryonic development. This indicates a progressive change of hormone sensitivity of absorptive cells during differentiation.

show abstract

Section: Expression Of Vitamin D Effects During Embryonic Developmentmentioning

confidence: 99%

Differential Response of Enterocytes to Vitamin D during Embryonic Development: Induction of Intestinal Inorganic Phosphate, D-Glucose and Calcium Uptake

Cross¹,

Peterlik²

1982

Horm Metab Res

View full text Add to dashboard Cite

show abstract

“…The observed effects of calcitriol on calcium transport therefore are likely to result from receptor-mediated changes in the activity of the calbindin gene. However, the present study shows that, although the vitamin D receptor is present already in undifferentiated day 15 embry¬ onic intestine (18), calcitriol-related calcium trans¬ port gradually declines with advancing epithelial maturation (Fig. 1).…”

Section: Discussionmentioning

confidence: 48%

“…T3-induced ct-methyl-glucoside uptake was highest on day 15, but was practically undetectable at the final stage of maturation during day [17][18][19][20] (Fig. 1).…”

Section: Resultsmentioning

confidence: 96%

Differentiation-dependent expression of calcitriol actions on absorptive processes in cultured chick intestine: modulation by triiodothyronine

Cross

Peterlik

1991

Acta Endocrinologica

View full text Add to dashboard Cite

Embryonic chick jejunum maintained in organ-culture exhibits a characteristic stage-specific pattern of responses to calcitriol and T3. Whereas induction of luminal Na+/inorganic phosphate and Na+/D-glucose transport by calcitriol was only possible at an advanced state of differentiation prior to hatching on day 20, the sterol induced cellular calcium transport with high efficiency even in undifferentiated enterocytes in day 15 embryonic intestine. T3 had no effect at all on calcium transport, but induced Na+/inorganic phosphate transport at all stages of epithelial maturation. In contrast, Na+/D\x=req-\ glucose transport was effectively induced by T3 only in relatively immature intestinal epithelium. T3, at a medium concentration of 10\m=-\8mol/l, in a permissive fashion potentiated the effects of calcitriol (10\ m=-\ 10\ m=-\ 10\ m=-\ 7mol/l) on calcium transport as well as on Na+/inorganic phosphate and Na+/D-glucose transfer. Thereby, T3 facilitated induction of transport activities by calcitriol against differentiation-related restraints. By facilitating the expression of genomic actions of calcitriol, T3 may thus play an important role in the regulation of calcium and phosphate metabolism.It has long been known that dysfunctions of the thyroid gland are frequently associated with dis¬ turbances of calcium and inorganic phosphate (P¡) homeostasis. It is, therefore, obvious that thyroid hormones must play an important role in mineral metabolism (reviewed in 1), although the actions at the cellular level constituting their function as cal¬ cium and P¡ regulating hormones are not clarified at all. Within the wide spectrum of thyroid hor¬ mone effects, a number of actions on bone, the kidney and small intestine resemble those of the classic calciotropic hormone, calcitriol (1,25-dihydroxyvitamin D3): Thus, thyroid hormones can stimulate bone résorption (2), enhance renal tubu¬ lar reabsorption of P¡ (3), and increase Na+ gradi¬ ent-driven uptake of P¡ by the small intestine (4). We want to emphasize that thyroid hormones may play an additional role in mineral metabolism owing to their ability to potentiate the genomic ef¬ fects of calcitriol on calcium and P¡ absorption by the small intestine: Utilizing an organ culture system of embryonic chick jejunum (5), we were able to show that the various genomic effects of calcitriol on intestinal transport of calcium (6-8) and P¡ (9) are markedly enhanced by triiodothyronine (10,11). In addition, calcitriol-related absorp¬ tion of D-glucose (12) can also be augmented by T3 in a synergistic fashion (11). We reported previ¬ ously that the expression of vitamin D3 action on these absorptive functions of enterocytes depends on the state of embryonic development and thus on the degree of epithelial cell differentiation (13). We therefore sought to explore the possibility that the stage-specific expression of calcitriol actions on in¬ testinal calcium uptake as well as on Na+/P¡ and Na+/D-glucose transport could be modulated by T,. Materials and MethodsFertilized eggs were ob...

show abstract

“…Although Moriuchi and DeLuca [17] demonstrated that intestinal calcium-binding protein (CaBP) does not appear in chicks until 1 day after hatching, Oku et al [16] showed that the 1,25(OH)• receptor appears in the duodenal cytosol in 15-day embryos and gradually increases until day 19. Moreover, Shimura et al [6] suggested that the intestinal 1,25(OH)2D3 receptor system becomes competent for intracellular transfer of 1,25(OH)2D3 before hatching.…”

Section: Discussionmentioning

confidence: 99%

Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol

et al. 1982

View full text Add to dashboard Cite

This study presents measurements of serum vitamin D metabolites, calcium and phosphorus as well as measurements of the equilibrium dissociation constant for duodenal 1,25(OH)2D3 receptor in 15-, 18-, 19-, and 20-day chick embryos in comparison to that in 1- and 118-day-old chicks and to vitamin D-deficient chicks. The present results showed that: (a) serum 1,25(OH)2D and 24,25(OH)2D levels rise from 15 and 18 to days 19 and 20 of embryonic development while serum phosphate levels are stable; (b) serum calcium levels rise at hatching to adult levels; (c) the duodenal 1,25(OH)2D3 receptor is detectable in 15-day-old embryo and has a Kd similar to that of 118-day-old vitamin D-replete chicks; and (d) the activity of 1,25(OH)2D3 receptor in chick duodenal cytosol is maximal at hatching.

show abstract

Development of 1,25-dihydroxycholecalciferol receptor in the duodenal cytosol of chick embryo.

Cited by 14 publications

References 11 publications

Differential Response of Enterocytes to Vitamin D during Embryonic Development: Induction of Intestinal Inorganic Phosphate, D-Glucose and Calcium Uptake

Differential Response of Enterocytes to Vitamin D during Embryonic Development: Induction of Intestinal Inorganic Phosphate, D-Glucose and Calcium Uptake

Differentiation-dependent expression of calcitriol actions on absorptive processes in cultured chick intestine: modulation by triiodothyronine

Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol

Contact Info

Product

Resources

About