Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol

Seino, Yoshiki; Yamaoka, Kanji; Ishida, Makoto; Yabuuchi, Hyakuji; Ichikawa, M.; Ishige, Hisako; Yoshino, Hidenori; Avioli, Louis V.

doi:10.1007/bf02411248

Cited by 55 publications

(10 citation statements)

References 20 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RIA has been developed by using antisera raised against 3 or 25 -hemisuccinate of 1,25 (OH)2D3 (18,19), but a good antibody with high sensitivity has not been obtained. RRA has been developed by many research groups (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16) and it is most popular as a routine method for determination of 1,25 (OH)2D in plasma because of its high sensitivity. In a previous paper (13), we have established a method for determination of 1,25(OH)2D using chick embryonal intestinal receptor.…”

Section: Discussionmentioning

confidence: 99%

“…However, since plasma levels of 1,25(OH)2D are extremely low (normal range: 20-80pg/ml) , it is very difficult to establish a simplified and precise method. Since high-performance liquid chromatography (HPLC) using an ultraviolet (UV) detector and gas-liquid chro matography/mass spectrometry (GC-MS) cannot be applied to the determination of 1,25(OH)2D in plasma because of their low sensitivity (5), radioreceptor assay (RRA) is now most popular for the purpose (6)(7)(8)(9)(10)(11)(12)(13). Cytosol-chromatin receptors (6-13) have been used for RRA.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Gross and Separative Determination of 1.ALPHA.,25-Dihydroxyvitamin D2 and D3 in Plasma Using Calf Thymus Receptor.

Masuda¹,

Okano²,

Kobayashi³

1993

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

View full text Add to dashboard Cite

SummaryDetermination of la,25-dihydroxyvitamin D [1,25(OH)2D, gross amounts of 1,25(OH)2D2 and 1,25(OH)2D3] and separative deter mination of 1,25(OH)2D2 and 1,25(OH)2D3 in plasma using calf thymus receptor have been investigated. A lipid extract from 1ml of plasma is applied to a Bond Elut C180H column and an eluate corresponding to 1,25 (OH)2D including both 1,25(OH)2D2 and 1,25(OH)2D3 is applied to calf thymus receptor to assay a gross amount of the two compounds. On the other hand, when separative assay of the two compounds is performed, the 1,25 (OH)2D eluate obtained from the Bond Elut C180H column is further applied to HPLC using a Zorbax SIL column with 5% isopro panol in methylene chloride as a developing solvent to separate the two compounds from one another. The separated eluates are independently applied to the receptor to assay the two compounds. Since less amounts of unknown components non-specifically bound to interfering concomi tants besides 1,25(OH)2D exist in the calf thymus receptor, complicated purification steps to eliminate the concomitants are unnecessary. The detection limit by this method is 1.25 pg/tube which is sensitive enough for a routine method to assay 1,25(OH)2D in plasma. Key Words vitamin D, 1,25-dihydroxyvitamin D, 1,25-dihydroxyvitamin D2, 1,25-dihydroxyvitamin D3, radioreceptor assay, calf thymus receptor Vitamin D (vitamin D is used as a general name for vitamins D2 and D3 and the same nomenclature system is also applied to the metabolites) is metabolized to in the liver and subsequently to la,25-dihy droxyvitamin D (1,25(OH)2D) or 24R,25-dihydroxyvitamin D (24,25(OH)2D) in the kidney (1, 2). 1,25(OH)2D is known as an active form of vitamin D which promotes intestinal calcium absorption and remodeling in bone to keep calcium homostasis. Recent investigations have revealed that 1,25(OH)2D has the activity

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Gross and Separative Determination of 1.ALPHA.,25-Dihydroxyvitamin D2 and D3 in Plasma Using Calf Thymus Receptor.

Masuda¹,

Okano²,

Kobayashi³

1993

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

View full text Add to dashboard Cite

show abstract

“…Radioreceptor assay of the transformation products with a la,25(OH)2D3 receptor (Seino et al 1982) prepared from foetal chicken intestine was carried out as reported previously (Sasaki et al 1991). The assay was performed to distinguish between lc~,25-dihydroxyvitamin D compounds and lfl,25-dihydroxyvitamin D compounds on the basis of purified transformation products (Ikekawa 1987).…”

Section: Methodsmentioning

confidence: 99%

Transformation of vitamin D3 to 1?,25-dihydroxyvitamin D3 via 25-hydroxyvitamin D3 using Amycolata sp. strains

Sasaki

Miyazaki

Saito

et al. 1992

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

To enzymatically synthesize active metabolites of vitamin D3, we screened about 500 bacterial strains and 450 fungal strains, of which 12 strains were able to convert vitamin D3 to 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] via 25-hydroxyvitamin D3 [25(OH)D3]. The conversion activity was only detected in strains belonging to the genus Amycolata among all the organisms tested. A preparative-scale conversion of vitamin D3 to 25(OH)D3 and 1 alpha,25(OH)2D3 in a 200-1 tank fermentor using A. autotrophica FERM BP-1573 was accomplished, yielding 8.3 mg 25(OH)D3/l culture and 0.17 mg 1 alpha,25(OH)2D3/l culture. A related compound, vitamin D2, could be also converted to 25-hydroxyvitamin D2 and 1 alpha,25-dihydroxyvitamin D2 using the same strain. The cytochrome P-450 of FERM BP-1573 was detected by reduced CO difference spectra in whole-cell suspensions. Vitamin D3 in the culture induced cytochrome P-450 and the conversion activity simultaneously, suggesting that the hydroxylation at C-25 of vitamin D3 and at C-1 of 25(OH)D3 originates from cytochrome P-450.

show abstract

“…Displacement assay of [26,27-3H]-1,25(OH)2D 3 from the chick intestinal cytosol receptor (Yamasa Japan) by 24aFz-homo-l,25(OH)zD 3 and 24Fz-l,25(OH)z(Me)2D 3 was performed as described elsewhere [19]. One-half milliliter of the chick intestinal cytosol receptor protein diluted to 0.5 mg protein/ml in TED Mo buffer A (10 mM Tris, 1.5 mM EDTA, 1 mM dithiothreitol, 10 mM NaMoO4, pH = 7.4) was added to 15 ~1 of ethanol (Nakarai, for analysis of hormones) dissolved [26,27-3H] -1,25(OH)2D 3 and various concentrations of test samples in an ice-cold bath.…”

Section: Binding Affinity For Chick Intestinal Receptormentioning

confidence: 99%

Calcium regulating activity of 24a-homo-24,24-difluoro-1?,25-dihydroxyvitamin D3 and 26,27-dimethyl-24,24-difluoro-1?,25-dihydroxyvitamin D3

et al. 1993

View full text Add to dashboard Cite

Two fluoro analogs of 1 alpha,25-dihydroxyvitamin D3 [1,25(OH)2D3], 24a-homo-24,24-difluoro-1 alpha,25-dihydroxyvitamin D3 [24aF2-homo-1,25(OH)2D3], and 26,27-dimethyl-24,24-difluoro-1 alpha,25-dihydroxyvitamin D3 [24F2-1,25(OH)2(Me)2D3] were examined for calcium (Ca)-regulating activity. The objective of the present study was to determine whether or not fluoro substitution at 24-position would alter activities of the original compounds, that is, 26,27-dimethyl 1 alpha,25-dihydroxyvitamin. D3[1,25(OH)2(Me)2D3] and 24-homo-1 alpha,25-dihydroxyvitamin D3 [24homo-1,25(OH)2D3], respectively. The relative activities of 24aF2-homo-1,25(OH)2D3, 24F2-1,25(OH)2(Me)2D3, and 1,25(OH)2D3 in competing with 1,25(OH)2D3 for binding to chick intestinal cytosol receptor were 0.28:0.5:1.0. The relative potencies of the same series of compounds in competition for the vitamin D-deficient rat serum binding sites were 0.04:0.15:1. Bone-resorbing activities of two fluoro analogs in cultures of neonatal mouse parietal bones were more potent than that of 1,25(OH)2D3. Similar results were recognized in stimulating activities of osteoclast-like cell formation. Responses of two fluoro analogs to intestinal Ca absorption were similar to that of 1,25(OH)2D3. The potencies of 1,25(OH)2D3 and its fluoro analogs in bone Ca mobilization were the highest with 1,25(OH)2D3, followed by 24F2-1,25(OH)2(Me)2D3 and 24aF2-homo-1,25(OH)2D3, in that order. From these results and the data of Paulson et al., fluoro substitution in 24-position of 1,25(OH)2D3 apparently does not alter their activities,hence, the fluoro substitution at 24-position of 1,25(OH)2D3 and the elongation of side chain of 1,25(OH)2D3 may not intensify Ca-regulating activity.

show abstract

Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol

Cited by 55 publications

References 20 publications

Gross and Separative Determination of 1.ALPHA.,25-Dihydroxyvitamin D2 and D3 in Plasma Using Calf Thymus Receptor.

Gross and Separative Determination of 1.ALPHA.,25-Dihydroxyvitamin D2 and D3 in Plasma Using Calf Thymus Receptor.

Transformation of vitamin D3 to 1?,25-dihydroxyvitamin D3 via 25-hydroxyvitamin D3 using Amycolata sp. strains

Calcium regulating activity of 24a-homo-24,24-difluoro-1?,25-dihydroxyvitamin D3 and 26,27-dimethyl-24,24-difluoro-1?,25-dihydroxyvitamin D3

Contact Info

Product

Resources

About