Development and validation studies for determination of phenylpropanoid‐substituted flavan‐3‐ols in semipurified extract of Trichilia catigua by high‐performance liquid chromatography with photodiode array detection

Abstract

The phenolic compounds are the main phytochemical constituents of the bark of Trichilia catigua and are commonly used for medicinal purposes. An HPLC method for the simultaneous quantification of phenolic compounds (procyanidin B2 (PB2), epicatechin (EPC), chinchonains Ia, Ib, IIa, IIb, catechin, and chrologenic acid) in T. catigua extract was developed and validated. A suitable chromatographic system was selected, which uses a gradient elution with methanol/ACN (75:25), and water both with 0.05% TFA, as mobile phase, column Luna, 280 nm, and flow 0.4 mL/min. Validation of the analytical method was based on the parameters: linearity, precision, LODs and LOQs, accuracy, robustness, and stability. The method showed linearity for PB2 and EPC, in the range 10-120 μg/mL with good correlation coefficients (>0.996). For precision, the repeatability ranged from 1.89 to 3.23%, and the values for accuracy for PB2 and EPC were 95 and 89%, respectively. The LODs and LOQs for PB2 were 1.36 and 4.12 μg/mL, and for EPC were 2.18 and 6.61 μg/mL, respectively. The method was robust under the conditions employed. The proposed method could be employed for quality assessment of T. catigua, as well as pharmaceutical products.

Anti-Diabetic Effects of the Ethyl-Acetate Fraction of Trichilia catigua in Streptozo-tocin-Induced Type 1 Diabetic Rats

“…The quality of the EAF was evaluated by measuring the amounts of procyanidin B2 (PB2) and epicatechin (EPC) in this fraction against analytical standards by HPLC analysis (Thermo ® , San Jose, CA, USA), according to a method developed and validated by our group, as previously described [16]. These standards were chosen for validation because they were available in the appropriate quantities at the laboratory.…”

“…The crude extract was dissolved in water, and EAF was isolated using an ethyl-acetate solution as previously described [13,16].…”

The exposure to Trichilia catigua (catuaba) crude extract impairs fertility of adult female rats but does not cause reproductive damage to male offspring

“…The drugs derived from plants naturally vary in their chemical composition, mainly due to climate and others (Kutchan, 2001), so the quality control of the plant raw material is important for monitoring the different lots to maintain quality (Longhini et al, 2013). The presence and the proportion of the chemical constituents are ensured by the quality control and facilitate future studies like bioavailability.…”

“…Longhini et al (2013) developed and validated an analytical method to quantify PB2 and EPC on T. catigua barks. Longhini et al (2013) developed and validated an analytical method to quantify PB2 and EPC on T. catigua barks.…”

“…After, the tubes were centrifuged (refrigerated microcentrifuge Eppendorf 5415R) at 4000 rpm, for 4 min at 5 1C, like Lopes et al (2009). The quantification of procyanidin B2 (PB2) and epicatechin (EPC) in a T. catigua crude extract was performed as Longhini et al (2013). The residue was reconstituted to 3 mL with methanol: water (2:8; v/v) and was eluted through the solid-phase extraction cartridge (SPE, Strata C18-E, Phenomenex s ) pre-conditioned with methanol and water, and diluted in 10 mL of methanol:water (2:8; v/v).…”