Anti-Diabetic Effects of the Ethyl-Acetate Fraction of Trichilia catigua in Streptozo-tocin-Induced Type 1 Diabetic Rats

Gomes, Rodrigo Mello; Paulo, Luis Fernando de; Panizzon, Cynthia Priscilla do Nascimento Bonato; Neves, Camila Quaglio; Cordeiro, Bruna Colombo; Zanoni, Jacqueline Nelisis; Francisco, Flávio Andrade; Piovan, Silvano; Mathias, Paulo Cézar de Freitas; Longhini, Renata; Mello, João Carlos Palazzo de; Oliveira, Júlio Cézar de; Pedrino, Gustavo Rodrigues; Reis, Ângela Adamski da Silva; Cecchini, Alessandra Lourenço; Natali, Maria Raquel Marçal

doi:10.1159/000478761

Cited by 21 publications

(21 citation statements)

References 32 publications

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“…Extracts rich in polyphenol compounds from several plants have been reported to be effective against cell damage mediated by reactive oxygen species by increasing antioxidant defenses and reducing hyperglycemia [ 35 ]. Fractions obtained using the ethyl acetate solvent have a high concentration of polyphenols, as has been obtained from Trichilia catigua [ 36 ], grape skin [ 37 ], and stevia leaves [ 22 , 38 ]. In addition, insulin-producing effects of polyphenols in chronic treatment in vivo , in pancreatic islet culture, and in insulin-producing cells [ 39 , 40 ] have been reported, but direct effects on isolated islets, such as those shown in the current work with a fraction of stevia, have not been previously reported.…”

Section: Discussionmentioning

confidence: 99%

Stevia Nonsweetener Fraction Displays an Insulinotropic Effect Involving Neurotransmission in Pancreatic Islets

Piovan

Pavanello

Peixoto

et al. 2018

International Journal of Endocrinology

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Stevia rebaudiana (Bert.) Bertoni besides being a source of noncaloric sweeteners is also an important source of bioactive molecules. Many plant extracts, mostly obtained with ethyl acetate solvent, are rich in polyphenol compounds that present insulinotropic effects. To investigate whether the nonsweetener fraction, which is rich in phenolic compounds isolated from Stevia rebaudiana with the solvent ethyl acetate (EAF), has an insulinotropic effect, including interference at the terminals of the autonomic nervous system of the pancreatic islets of rats. Pancreatic islets were isolated from Wistar rats and incubated with EAF and inhibitory or stimulatory substances of insulin secretion, including cholinergic and adrenergic agonists and antagonists. EAF potentiates glucose-stimulated insulin secretion (GSIS) only in the presence of high glucose and calcium-dependent concentrations. EAF increased muscarinic insulinotropic effects in pancreatic islets, interfering with the muscarinic receptor subfamily M3. Adrenergic inhibitory effects on GSIS were attenuated in the presence of EAF, which interfered with the adrenergic α 2 receptor. Results suggest that EAF isolated from stevia leaves is a potential therapy for treating type 2 diabetes mellitus by stimulating insulin secretion only in high glucose concentrations, enhancing parasympathetic signal transduction and inhibiting sympathetic signal transduction in beta cells.

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Section: Discussionmentioning

confidence: 99%

Stevia Nonsweetener Fraction Displays an Insulinotropic Effect Involving Neurotransmission in Pancreatic Islets

Piovan

Pavanello

Peixoto

et al. 2018

International Journal of Endocrinology

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“…Measurements of islet area were performed by Image-Pro Plus using specific area measurement tools. The relative beta-cell mass per pancreas area, was determined by point counting morphometry on each pancreas section immunostained for insulin according previous descriptions ( Xu et al, 1999 ; Rafacho et al, 2009 ; Malta et al, 2016 ; Gomes et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Aerobic Exercise Training Attenuates Tumor Growth and Reduces Insulin Secretion in Walker 256 Tumor-Bearing Rats

et al. 2018

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Aerobic exercise training can improve insulin sensitivity in many tissues; however, the relationship among exercise, insulin, and cancer cell growth is unclear. We tested the hypothesis that aerobic exercise training begun during adolescence can attenuate Walker 256 tumor growth in adult rats and alter insulin secretion. Thirty-day-old male Wistar rats engaged in treadmill running for 8 weeks, 3 days/week, 44 min/day, at 55–65% VO2max until they were 90 days old (TC, Trained Control). An equivalently aged group was kept inactive during the same period (SC, Sedentary Control). Then, half the animals of the SC and TC groups were reserved as the control condition and the other half were inoculated with Walker 256 cancer cells, yielding two additional groups (Sedentary Walker and Trained Walker). Zero mortalities were observed in tumor-bearing rats. Body weight (BW), food intake, plasma glucose, insulin levels, and peripheral insulin sensitivity were analyzed before and after tumor cell inoculation. We also evaluated tumor growth, metastasis and cachexia. Isolated pancreatic islets secretory activity was analyzed. In addition, we evaluated mechanic sensibility. Our results showed improved physical performance according to the final workload and VO2max and reduced BW in trained rats at the end of the running protocol. Chronic adaptation to the aerobic exercise training decreased tumor weight, cachexia and metastasis and were associated with low glucose and insulin levels and high insulin sensitivity before and after tumor cell inoculation. Aerobic exercise started at young age also reduced pancreatic islet insulin content and insulin secretion in response to a glucose stimulus, without impairing islet morphology in trained rats. Walker 256 tumor-bearing sedentary rats also presented reduced pancreatic islet insulin content, without changing insulin secretion through isolated pancreatic islets. The mechanical sensitivity test indicated that aerobic exercise training did not cause injury or trigger inflammatory processes prior to tumor cell inoculation. Taken together, the current study suggests that aerobic exercise training applied during adolescence may mitigate tumor growth and related disorders in Walker 256 tumor-bearing adult rats. Improved insulin sensibility, lower glucose and insulin levels and/or reduced insulin secretion stimulated by glucose may be implicated in this tumor attenuation.

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“…Furthermore, the evaluation of histopathological studies demonstrated that activate AMPK signaling pathway attenuate the kidney tissue damage that is caused by STZ-diabetes. STZ-induced type 1 diabetic rats are associated with severe decrease of body weight caused by hyperglycemia, loss of tissue proteins (14), muscular tissue and adipose tissue (15). Additionally, the increase in the levels of serum uric acid and creatinine (16) and the decrease in the levels of serum albumin and total protein (17) have been associated with diabetic kidney disease.…”

Section: Discussionmentioning

confidence: 99%

Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through activation of AMPK signaling pathway

Hasanvand¹,

Amini‐Khoei²,

Jahanabadi³

et al. 2017

J Nephropathol

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Background: Nephropathy is the main problem of diabetes and can be classified into several phases according to the presence of albuminuria. Adenosine monophosphate-activated protein kinase (AMPK) operates as a sensor of energy charge. Objectives: The aim of our study was to evaluate the reno-protective properties of AMPK signaling pathway against streptozotocin (STZ)-induced nephropathy in the rat. Materials and Methods: Forty male Wistar rats were randomly distributed into four groups. Group 1 was normal rats (N group); group 2 was diabetic rats (D group); group 3 received diabetic rats + metformin (DM group), and group 4 received giabetic rats + metformin + dorsomorphin (DMD group). Serum albumin, uric acid, total protein and creatinine for estimation of renal injury were measured. Finally, the histological study was evaluated. Results: Reduction of body weight, albumin and total protein in the diabetic rat was reversed by metformin administration. Our results showed that serum uric acid and creatinine were significantly increased in diabetic rats and decreased after treatment with metformin in diabetic rats. AMPK improved the histopathology and morphological changes in STZinduced diabetic rats. Administration of dorsomorphin (AMPK inhibitor) with metformin can reverse the beneficial effects of AMPK. Conclusions: AMPK signaling pathway ameliorates diabetic nephropathy by modifications of serum albumin, uric acid, total protein, creatinine and attenuation of kidney damage.

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Anti-Diabetic Effects of the Ethyl-Acetate Fraction of Trichilia catigua in Streptozo-tocin-Induced Type 1 Diabetic Rats

Cited by 21 publications

References 32 publications

Stevia Nonsweetener Fraction Displays an Insulinotropic Effect Involving Neurotransmission in Pancreatic Islets

Stevia Nonsweetener Fraction Displays an Insulinotropic Effect Involving Neurotransmission in Pancreatic Islets

Aerobic Exercise Training Attenuates Tumor Growth and Reduces Insulin Secretion in Walker 256 Tumor-Bearing Rats

Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through activation of AMPK signaling pathway

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