Development and Validation of UV Spectrophotometric Method for Doxazosin Mesylate in Bulk and Tablets

Abstract

Doxazosin mesylate (DX) is an antihypertensive agent belonging to BCS class II. There is no reported literature available on development of spectrophotometric method for its analysis in bulk and pharmaceutical dosage forms using 0.1N HCl. Thus, attempt was made to develop and validate a new UV spectrophotometric method using 0.1N HCl as the solvent for its quantitative estimation in tablets that would be fast, simple, accurate, and sensitive. DX had a maximum absorbance wavelength of 245 nm and was linear with a correlation coefficient (R2) of 0.9981 across the concentration range of 2-14 μg/mL. The present method was utilized to determine the drug content of two commercial brands namely; brand I and brand II. The estimated amount of DX was 99.13% and 99.02% in these brands, respectively. The proposed method generated results that confirm the label claim and was accurate, precise, sensitive, and rugged during its validation study. The accuracy of the technique was tested using recovery research at three different levels, namely 80%, 100%, 120%, and the percent recovery rate was determined between 98% and 102% suggesting that the proposed approach is accurate. Precision and robustness were within the acceptable limits, complying with ICH standards. The proposed method could be used to quantify DX in API and dosage forms.

Potential Anti-osteoporosis compounds from Zingiber officinale: A Molecular Docking and Pharmacokinetics Prediction

“…Furthermore, another compound predicted to have inhibitory action against cathepsin K is (S)-8-gingerol. Gingerols are the primary metabolites in Zingiber officinale and were reported to have many protective and therapeutic effects 49 . 8-Gingerol took 3.20% of white ginger and 4.64% of yellow ginger extract composition, while 6-gingerol was the most abundant, took up 27.56% and 33.96% of sample composition in white and yellow ginger extract, respectively 50 .…”

Exploring Cosolvency in Analytical Method Development and Validation of Poorly Aqueous Soluble Candesartan Cilexetil in Bulk and Dosage Forms

“…The proposed method was validated for the following parameters as per International Council for Harmonisation (ICH) guidelines [16][17][18][19][20] . Accuracy: Accuracy of the method was estimated by standard addition recovery method.…”

Evaluation of ginger genotypes for commercial cultivation in Mizoram and future prospects

“…5 ml was taken and volume was raised to 25 ml with methanol and the reading was taken at 282 nm wavelength using UV/VIS Spectrophotometer against methanol as blank. Percentage gingerol present in ginger extract was calculated from reference UV Spectra of Standard Gingerol (Shukla et al, 2012). The mean data were statistically analysed using IBM SPSS Software, Version 26.…”