Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Hesperidin and Diosmin in the Pharmaceutical Dosage Form

Abstract

A simple, rapid, precise and highly selective spectrophotometric method was developed for simultaneous estimation of Hesperidin and Diosmin in tablet dosage form. This method, involves the measurement of absorbances of Hesperidin and Diosmin at the wavelengths of 285 nm ( max of Hesperidin) and 268 nm ( max , of Diosmin). The UV spectra's of Hesperidin and Diosmin prepared in different solvents water, methanol, and acetonitrile and 0.2 N sodium hydroxide were recorded. These two drugs showed good absorbances when dissolved in 0.2 N NaOH. Hence 0.2 N NaOH was selected as the solvent for the method. Two wavelengths 285 and 268 nm were selected which are max of two drugs Hesperidin and Diosmin, respectively. Different concentrations of Hesperidin (5-50 g/mL) and Diosmin (2-24 g/mL) and a mixture of Hesperidin and Diosmin were prepared, scanned and absorbances were noted at the two wavelengths were fixed for the study. The method showed good reproducibility and recovery with % RSD less than 2. The LOD of Hesperidin and Diosmin was found to be 0.139 g/mL and 0.048 g/ml and LOQ of Hesperidin and Diosmin was found to be 0.42 g/mL and 0.147 g/mL, respectively. Thus the proposed method was found to be rapid, specific, precise, accurate and cost effective quality control tool for the routine analysis of Hesperidin and Diosmin in bulk and combined dosage form.

Biopharmaceutical and marketing evaluation of diosmin- and hesperidin-containing products on the Bulgarian market

“…1A) and the flavanon glycoside (Fig. 1B) possess vasoprotective and venotonic properties (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Diosmin differs from hesperidin by a double bond present in the structure of the former between C 2 and C 3 ( Fig.…”

“…1) (1)(2)(3). Although both glycosides are naturally occurring in the families of Rutaceae, Lamiaceae, Apiaceae, Scrophulariaceae (S. nadosa) (2)(3)(4)(5)(6)(7)(8)(9)(10), the industrial sector uses semisynthetic diosmin derived from hesperidin by a suitable oxidation reaction (I 2 /pyridine, I 2 /dimethylsulfoxide or other) (1)(2)(3)11,(15)(16)(17)(18). Hesperidin is most commonly isolated from dry orange or citrus peels (Citrus sinesis, L., Citrus aurantium, L., Citrus lemon, L., and other Citrus species) (3,8,10).…”

Oxidative Stress-Based Photodynamic Therapy with Synthetic Sensitizers and/or Natural Antioxidants

“…In human nutrition, it contributes to maintenance of the integrity of blood vessels. Hesperidin has similar structure and properties with naringin, which is difficult to be separated from citrics [102]. In the same manner, diosmin is a hesperidin derivative, from the flavonoid family.…”

“…In the same manner, diosmin is a hesperidin derivative, from the flavonoid family. As a synthetic drug, it is used for venous disease and for hemoroidal diseases [102]. Hesperetin, as a flavonoid from oranges and grapfruits, is a good protector of heart disease.…”

Development and validation of a fast and simple RP-HPLC method for the determination of diosmin and hesperidin in combined tablet dosage form

“…There are many published chemical analytical methods for the simultaneous determination and quantification of these compounds in food products and solid pharmaceutical dosage forms. Srilatha et al [4] described a UV spectrophotometric method for the simultaneous determination of diosmin and hesperidin in pharmaceutical preparations. Moldovan et al [5] published a spectrophotometric method for diosmin determination only, based on the reaction of diosmin with 4aminoantipyrine in the presence of hexacyanoferrate (III) in an alkaline medium and the subsequent formation of a purple colored product with λmax at 524.5 nm.…”