Development and validation of UPLC-MS/MS assay for quantification of cladrin: Absolute bioavailability and dose proportionality study in rats

Rashid, Mamunur; Singh, Sandeep K.; Malik, Monika; Jahan, Sadaf; Chaturvedi, Swati; Taneja, Isha; Raju, K. Kanaka; Naseem, Zaiba; Gayen, Jiaur R.; Wahajuddin, Muhammad

doi:10.1016/j.jpba.2018.01.044

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…Since afrormosin has a methoxy and a hydroxy group on the A‐ring and a single methoxy substituent on the B‐ring, it could be excluded from the list of possible structures (Figure ). Comparing the MS/MS spectra of cladrin and 2′‐methoxy formononetin with our data, the peaks at m/z 163.0, 148.1, and 137.1 could be observed in all cases indicating the double methoxy substitution of the B‐ring; however, inspecting fragments in higher m/z regions, our detected peaks were clearly in agreement with the ones of the 2′‐methoxy formononetin. 2′‐Methoxy formononetin has been isolated from Dalbergia parviflora L .…”

Section: Resultssupporting

confidence: 82%

Phytochemical analysis of Ononis arvensis L. by liquid chromatography coupled with mass spectrometry

et al. 2019

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Ononis arvensis L. can be found overall in Europe and is used to treat infections of the urinary tract and skin diseases in ethnopharmacology. Flavonoids, hydroxycinnamic acids, oxycoumarin, scopoletin and scopolin, phytosterols, lectins, and some selected isoflavonoids were identified in O. arvensis till date; however, there is a lack of the detailed investigation of the isoflavonoid profile of the plant. With the application of high‐resolution tandem mass spectrometry, the fragmentation patterns of isoflavonoid derivatives found in O. arvensis roots and aerial parts were investigated and discussed. Isoflavonoid glucosides, glucoside malonates, aglycones, and beta amino acid derivatives were characterized, among which homoproline isoflavonoid glucoside esters were described for the first time. Besides the known isoflavonoid aglycones described earlier in other Ononis species, two 2′‐methoxy isoflavonoid derivatives were detected. The presence of licoagroside B was verified, and its structure was also corroborated by NMR experiments. Altogether, the high‐resolution fragmentation pattern of 47 isoflavonoids and glycosides is presented, and their relative quantity in the roots and the aerial parts can be evaluated. Based on this information, the chemotaxonomic relation of Ononis species and the biosynthesis of their compounds could be comprehended to a greater depth.

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Section: Resultssupporting

confidence: 82%

Phytochemical analysis of Ononis arvensis L. by liquid chromatography coupled with mass spectrometry

et al. 2019

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“…In contrast to high passive permeability as described earlier, the oral exposure of WA was found to be very low which is also seen with other natural (Singh, Tewari, Pradhan, & Jain, ) and synthetic drugs (Singh, Raju, Nafis, Puri, & Jain, ). The possible reason for low oral exposure of WA could be attributed to low aqueous solubility, high first‐pass metabolism, saturation in gastric environment, and intestinal bacterial biotransformation (Rashid et al, ). However, more studies are obligatory to explore the poor oral exposure of WA in rats.…”

Section: Resultsmentioning

confidence: 99%

“…The possible reason for low oral exposure of WA could be attributed to low aqueous solubility, high first-pass metabolism, saturation in gastric environment, and intestinal bacterial biotransformation (Rashid et al, 2018). However, more studies are obligatory to explore the poor oral exposure of WA in rats.…”

Section: Pharmacokinetics Studymentioning

confidence: 99%

Determination of permeability, plasma protein binding, blood partitioning, pharmacokinetics and tissue distribution of Withanolide A in rats: A neuroprotective steroidal lactone

Singh

Valicherla

Joshi

et al. 2018

Drug Development Research

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Preclinical Research & Development Withanolide A (WA), a steroidal lactone is a major bioactive constituent of Withania somnifera (L.) with remarkable neuropharmacological activity. In this study, we investigated the permeability, plasma protein binding (PPB), blood partitioning, intravenous (i.v.), and oral pharmacokinetics as well as i.v. tissue distribution (TD) of pure WA in a rat model. The PPB, RBCs partitioning, and permeability of WA were determined by Ultra Performance Liquid Chromatography (UPLC) method. However, the pharmacokinetics and TD of WA were evaluated by validated and sensitive liquid chromatography coupled mass spectrometry (LC‐ESI‐MS/MS) method. The PPB and permeability of WA were determined by equilibrium dialysis and parallel artificial membrane permeability assay method, respectively. The results demonstrated that WA has high PPB and passive permeability. Furthermore, WA was found to have fast equilibration between RBCs and plasma. Following i.v. (2 mg/kg) and per‐oral (25 mg/kg) administration of WA, the max concentration (Cmax) in plasma was found as 85.53 ± 6.54 and 48.04 ±5.78 ng/mL, respectively. The TD study results indicated that WA has a rapid and wide TD. The maximum concentration in various tissues was found in following order: Clung > Cliver > Ckidney ≈ Cspleen > Cheart > Cbrain. The preclinical in vitro, as well as pharmacokinetics and TD results, are anticipated to support the future preclinical and clinical application of WA.

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“… 141 And oxidative stress in osteoblasts under high glucose conditions can cause autophagy defects, which may be the pathogenesis of diabetic osteoporosis, Wang et al reported that Timosaponin BII can inhibit the phosphorylation of mTOR and downstream factor NF-κB, activate autophagy through mTOR/NF-κB signaling pathway, reduce oxidative stress and OBs apoptosis induced by high glucose, and alleviate the deterioration of tibial microstructure in diabetic rats. 142 Cladrin are the main bioactive components found in the stem bark of purple rivet, which can promote the proliferation and differentiation of OBs, 143 , 144 Peng et al showed that the apoptosis rate of OBs treated with Cladrin in dexamethasone environment was significantly lower than that of OBs without Cladrin treatment, and the level of p-AMPK/T-AMPK was significantly up-regulated, the AMPK/mTOR signaling pathway was significantly involved in Cladrin-induced autophagy in OBs under dexamethasone. 144 Aucubin, an iridoid glycoside mainly found in Chinese herbal medicine, can enhance OBs autophagy through the AMPK signaling pathway to counteract steroid-induced apoptosis.…”

Section: Natural Compounds Regulate Obs and Ocs Autophagy Signaling P...mentioning

confidence: 99%

The Potential of Natural Compounds Regulating Autophagy in the Treatment of Osteoporosis

Zhao,

Qu,

Zhao

et al. 2023

JIR

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The maintenance of bone homeostasis is dynamically regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. Abnormal differentiation of osteoclast and insufficient osteoblast production can cause bone diseases such as osteoporosis. As one of the highly conserved catabolic pathways in eukaryotic cells, autophagy plays an important role in maintaining cell homeostasis, stress injury repair, proliferation and differentiation. Numerous studies have found that autophagy activity is essential for the survival, differentiation and function of bone cells, and that regulation of autophagy can affect the metabolism of osteoblasts and osteoclasts, thus affecting bone homeostasis. Therefore, using autophagy as a theme, this review outlines the basic process of autophagy, the relationship between autophagy and osteoblasts and osteoclasts, and summarizes the latest research progress of common autophagic signaling pathways in osteoblasts and osteoclasts. The regulatory effects of protein molecules and natural compounds on the autophagy pathway of osteoblasts and osteoclasts discovered in current research are summarized and discussed. This will help to further clarify the mechanism of osteoporosis, understand the relationship between autophagy and osteoporosis, and propose new therapeutic strategies and new ideas for anti-osteoporosis.

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Development and validation of UPLC-MS/MS assay for quantification of cladrin: Absolute bioavailability and dose proportionality study in rats

Cited by 9 publications

References 27 publications

Phytochemical analysis of Ononis arvensis L. by liquid chromatography coupled with mass spectrometry

Phytochemical analysis of Ononis arvensis L. by liquid chromatography coupled with mass spectrometry

Determination of permeability, plasma protein binding, blood partitioning, pharmacokinetics and tissue distribution of Withanolide A in rats: A neuroprotective steroidal lactone

The Potential of Natural Compounds Regulating Autophagy in the Treatment of Osteoporosis

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