Development and validation of stability indicating HPLC and HPTLC methods for determination of sulpiride and mebeverine hydrochloride in combination

Naguib, Ibrahim A.; Abdelkawy, Mohamed

doi:10.1016/j.ejmech.2010.05.021

Cited by 37 publications

(29 citation statements)

References 42 publications

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“…MEB and SUL were determined in their binary mixture via derivative spectroscopy [3,4], first derivative synchronous fluorescence spectroscopy [5]. TLC- densitometry [4], HPLC [4-6] and chemometric techniques [7]. …”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Walash

El-Din

El‐Enany

et al. 2012

Chemistry Central Journal

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A new simple, rapid and sensitive reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of sulpiride (SUL) and mebeverine Hydrochloride (MEB) in the presence of their impurities and degradation products. The separation of these compounds was achieved within 6 min on a 250 mm, 4.6 mm i.d., 5 m particle size Waters®-C18 column using isocractic mobile phase containing a mixture of acetonitrile and 0.01 M dihydrogenphosphate buffer (45:55) at pH = 4.0. The analysis was performed at a flow rate of 1.0 mL/min with fluorescence-detection at excitation 300 nm and emission at 365 nm. The concentration-response relationship was linear over a concentration range of 10- 100 ng/mL for both MEB and SUL with a limit of detection 0.73 ng/mL and 0.85 ng/mL for MEB and SUL respectively. The proposed method was successfully applied for the analysis of both MEB and SUL in bulk with average recoveries of 100.22 ± 0.757% and 99.96 ± 0.625% respectively, and in commercial tablets with average recoveries of 100.04 ± 0.93% and 100.03 ± 0.376% for MEB and SUL respectively. The proposed method was successfully applied to the determination of MEB metabolite (veratic acid) in real plasma simultaneously with SUL. The mean% recoveries (n = 3) for both MEB metabolite (veratic acid) and SUL were 100.36 ± 2.92 and 99.06 ± 2.11 for spiked human plasma respectively. For real human plasma, the mean% recoveries (n = 3) were and respectively.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Walash

El-Din

El‐Enany

et al. 2012

Chemistry Central Journal

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“…British pharmacopoeia [7] reported non aqueous titration methods for determination each of the studied drugs in its dosage form; also USP [8] reported the same technique for determination of DF, MET and METB. MEH was recently determined by several techniques including, RP-HPLC [9-12], spectrophotometric, [13] and HPTLC [12] methods. MET was determined in plasma [14-16], blood [17] fish muscles [18], procine liver [19] and in different pharmaceutical preparations using different HPLC techniques, [20-23].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods

Abdelaleem

Abdelwahab

2012

Chemistry Central Journal

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Background Metronidazole (MET) and Diloxanide Furoate (DF), act as antiprotozoal drugs, in their ternary mixtures with Mebeverine HCl (MEH), an effective antispasmodic drug. This work concerns with the development and validation of two simple, specific and cost effective methods mainly for simultaneous determination of the proposed ternary mixture. In addition, the developed multivariate calibration model has been updated to determine Metronidazole benzoate (METB) in its binary mixture with DF in Dimetrol ® suspension. Results Method (I) is the mean centering of ratio spectra spectrophotometric method (MCR) that depends on using the mean centered ratio spectra in two successive steps that eliminates the derivative steps and therefore the signal to noise ratio is enhanced. The developed MCR method has been successfully applied for determination of MET, DF and MEH in different laboratory prepared mixtures and in tablets. Method (II) is the partial least square (PLS) multivariate calibration method that has been optimized for determination of MET, DF and MEH in Dimetrol ® tablets and by updating the developed model, it has been successfully used for prediction of binary mixtures of DF and Metronidazole Benzoate ester (METB) in Dimetrol ® suspension with good accuracy and precision without reconstruction of the calibration set. Conclusion The developed methods have been validated; accuracy, precision and specificity were found to be within the acceptable limits. Moreover results obtained by the suggested methods showed no significant difference when compared with those obtained by reported methods. Graphical Abstract

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“…[1][2][3][4][5][6][7] Stability study and quantitative determination of mebeeverine hydrochloride. [8] UV spectrophotometric method was developed for Mebeverine HCl.…”

Section: Fig1 Structure Of Mebeverine Hydrochloridementioning

confidence: 99%

Method Development and Validation of Mebeverine HCL in Bulk Drugs by Rp-HPLC Method

Senthilkumar¹

2017

WJPPS

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Development and validation of stability indicating HPLC and HPTLC methods for determination of sulpiride and mebeverine hydrochloride in combination

Cited by 37 publications

References 42 publications

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods

Method Development and Validation of Mebeverine HCL in Bulk Drugs by Rp-HPLC Method

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