Development and Validation of Spectrophotometric Methods for the Determination and Spectroscopic Characterization of Vildagliptin Using Ii -Acceptors in Pharmaceutical Preparations

Abstract

Three simple, quick and sensitive methods are described for the spectrophotometric determination of vildagliptin (VLD) in pharmaceutical preparations. The methods are based on formation of colored charge transfer (CT) complexes between VLD as n-electron donor and chloranilic acid (CA), tetrachloro-1,4-benzoquinone (p-chloranil), 7,7,8,8-tetracyanoquinodimethane (TCNQ) as π-acceptors. The colored products were quantitated spectrophotometrically at 520, 535 and 842 nm for CA, p-chloranil and TCNQ methods, respectively. Optimization of different experimental conditions were investigated. Beer's law was obeyed in the concentration range of 20-250 µg mL -1 , 25-400 µg mL -1 and 20-500 µg mL -1 for CA, p-chloranil and TCNQ methods, respectively. The formation of the CT-complexes and the sites of interactions were confirmed by elemental analysis using IR, 1 H NMR spectroscopy. Validity of the methods in terms of specificity, linearity, accuracy, precision, robustness, limit of detection and limit of quantitation were evaluated. Good recoveries were obtained for all of the methods indicating that no interference was observed from concomitants usually present in dosage forms. The methods were applied successfully to the determination of VLD in pharmaceutical preparations.

Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma

“…It could be taken alone or with other antidiabetic medications 1 . Various methods have been published for the estimation of VIL using UV spectrophotometric 2,3 , spectrofluorimetric 4,5 , liquid chromatographic methods [6][7][8][9][10][11] and voltammetric method 12 .…”

Chromatographic separation of vildagliptin and l‐proline as in‐process impurity with the application of Youden's test and statistical analysis to test the robustness of the HPLC method

“…Vildagliptin is not listed in any pharmacopeia. A literature survey revealed that the determination of vildagliptin alone is performed by HPLC in plasma and pharmaceutical formulations , spectrophotometry and spectroflourimetry using the charge transfer complex technique with chloranilic acid, p ‐chloranil and 7,7,8,8‐tetracyanoquinodimethane . Spectrophotometric determinations have been reported using 1,2‐naphthoquinone‐4‐sulfonic acid and 4‐chloro‐7‐nitrobenzofurazan and after reaction with bromocresol green and bromothymol blue .…”

Determination on Vildagliptin in Rat Plasma by Capillary Electrophoresis Tandem Mass Spectrometry: It’s Application to Pharmacokinetic Study

“…[2][3][4][5] Development of quantitative analytical method for the estimation of vildagliptin in plasma is very important for studying pharmacokinetic effects and drug-drug interactions. Different analytical methods have been described for the quantification of vildagliptin from formulations and biological samples, including spectrophotometric methods, [6][7] HPLC, [8][9][10][11][12] GC-MS, 13 and capillary electrophoresis. 14 These methods are not sensitive for the quantification in terminal plasma concentration of vildagliptin during pharmacokinetic studies comprising lower dose of vildagliptin.…”