Development and validation of simple and sensitive HPLC-UV method for ethambutol hydrochloride detection following transdermal application

Anjani, Qonita Kurnia; Sabri, Akmal Hidayat Bin; Donnelly, Ryan F.

doi:10.1039/d1ay01414e

Cited by 9 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2. The results suggest that the calibration curves for both drugs exhibited a linear response with the regression coefficient (R 2 ) ≥ 0.998 over the concentration range analysed [16,18,19]. This is the first report of an HPLC method for the determination of DPA and CUR together.…”

Section: Chromatographic Methods Developmentmentioning

confidence: 66%

“…The LOD and LOQ of this method and the characteristics of the calibration curve obtained, including the slope, y-intercept and coefficient of correlation, are presented in Table 2 . The results suggest that the calibration curves for both drugs exhibited a linear response with the regression coefficient (R 2 ) ≥ 0.998 over the concentration range analysed [ 16 , 18 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

et al. 2022

Self Cite

View full text Add to dashboard Cite

Curcumin (CUR) and D-panthenol (DPA) have been widely investigated for wound-healing treatment. In order to analyse these two compounds from a dosage form, such as polymer-based wound dressings or creams, an analytical method that allows the quantification of both drugs simultaneously should be developed. Here, we report for the first time a validated high-performance liquid chromatographic (HPLC) method coupled with UV detection to quantify CUR and DPA based on the standards set by the International Council on Harmonization (ICH) guidelines. The separation of the analytes was performed using a C18 column that utilised a mobile phase consisting of 0.001% v/v phosphoric acid and methanol using a gradient method with a run time of 15 min. The method is linear for drug concentrations within the range of 0.39–12.5 μg mL−1 (R2 = 0.9999) for CUR and 0.39–25 μg mL−1 for DPA (R2 = 1). The validated method was found to be precise and accurate. Moreover, the CUR and DPA solution was found to be stable under specific storage conditions. We, therefore, suggest that the HPLC-UV method developed in this study may be very useful in screening formulations for CUR and DPA within a preclinical setting through in vitro release studies.

show abstract

Section: Chromatographic Methods Developmentmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The recovery was within the 100 ± 10% limit reported in the OECD guideline, and RSD% values are below the maximum limit of 15%. These suggest that the extraction procedure was highly efficient, consistent, precise and reproducible [ 71 , 72 , 73 ].…”

Section: Resultsmentioning

confidence: 99%

Fluorescence-Coupled Techniques for Determining Rose Bengal in Dermatological Formulations and Their Application to Ex Vivo Skin Deposition Studies

et al. 2023

Self Cite

View full text Add to dashboard Cite

Rose Bengal (RB) is a fluorescent dye with several potential biomedical applications, particularly in dermatology. Due to RB’s poor physicochemical properties, several advanced delivery systems have been developed as a potential tool to promote its permeation across the skin. Nevertheless, no validated quantitative method to analyse RB within the skin is described in the literature. Considering RB exhibits a conjugated ring system, the current investigation proposes fluorescence-based techniques beneficial for qualitatively and quantitatively determining RB delivered to the skin. Notably, the development and validation of a fluorescence-coupled HPLC method to quantify RB within the skin matrix are herein described for the first time. The method was validated based on the ICH, FDA and EMA guidelines, and the validated parameters included specificity, linearity, LOD, LLOQ, accuracy and precision, and carry-over and dilution integrity. Finally, the method was applied to evaluate RB’s ex vivo permeation and deposition profiles when loaded into dermatological formulations. Concerning qualitative determination, multiphoton microscopy was used to track the RB distribution within the skin strata, and fluorescence emission spectra were investigated to evaluate RB’s behaviour when interacting with different environments. The analytical method proved specific, precise, accurate and sensitive to analyse RB in the skin. In addition, qualitative side-analytical techniques were revealed to play an essential role in evaluating the performance of RB’s dermatological formulation.

show abstract

“…Standard solutions at different concentration were employed to check the linearity of the analytical method. 54 Calibration curves were generated by analysing the peak area against drug concentration using least squares linear regression. The solution of containing TZ and LD was prepared in concentration of 0.79, 1.56, 3.125, 6.25, 12.5, 25, 50, 100 μg ml −1 .…”

Section: Methodsmentioning

confidence: 99%

An isocratic RP-HPLC-UV method for simultaneous quantification of tizanidine and lidocaine: application to in vitro release studies of a subcutaneous implant

Picco,

Anjani,

Donnelly

et al. 2024

Anal. Methods

Self Cite

View full text Add to dashboard Cite

show abstract

Development and validation of simple and sensitive HPLC-UV method for ethambutol hydrochloride detection following transdermal application

Abstract: A new high-performance liquid chromatographic method coupled with UV detection (HPLC-UV) to quantify ethambutol (ETH) post permeation studies following microneedle administration has been developed. This method involves the derivatization of...

Cited by 9 publications

References 28 publications

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

Fluorescence-Coupled Techniques for Determining Rose Bengal in Dermatological Formulations and Their Application to Ex Vivo Skin Deposition Studies

An isocratic RP-HPLC-UV method for simultaneous quantification of tizanidine and lidocaine: application to in vitro release studies of a subcutaneous implant

Contact Info

Product

Resources

About