Development and validation of RP-HPLC assay for levofloxacin in rat plasma and saliva: Application to pharmacokinetic studies

Sheikh, N. A.; Tripathi, Alok Shiomurti; Chitra, V.; Choudhury, Avijit; Dewani, Anil P.

doi:10.5897/ajpp11.001

Cited by 5 publications

(3 citation statements)

References 12 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Levofloxacin hemihydrate is odourless drug. Methods for quantitative analysis of Levofloxacin by HPLC [1][2][3][4][5][6][7], by UV [8][9][10] spectroscopy in single as well as in combination, are available in the literature. There are no methods available for quantitative analysis by UPLC.…”

Section: Introductionmentioning

confidence: 99%

A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form: Application to Pharmaceutical Analysis

Dabhi

Parmar

Patel

et al. 2013

Chromatography Research International

View full text Add to dashboard Cite

A reliable and sensitive isocratic stability indicating RP-UPLC method has been developed and validated for quantitative analysis and content uniformity study of levofloxacin hemihydrate in tablets. An isocratic method for analysis of levofloxacin hemihydrate was archived on ACQUITY UPLC BEH C18 (100*2.1) mm particle size 1.7 μ columns within shorter runtime of 4 min with a flow rate of 0.400 mL/min and using a photodiode array detector to monitor the eluate at 294 nm. The mobile phase consisted of acetonitrile-buffer (23 : 77 v/v), (buffer: 20 mM K2HPO4 + 1 mL triethylamine in 1 L water, pH=2.50 by orthophosphoric acid). Response was a liner function of drug concentration in the range of 0.5–80 μg/mL (r2=0.999) with a limit of detection and quantification of 0.1 and 0.5 μg/mL, respectively. Accuracy (recovery) was between 99.77% and 101.55%. The drug was subjected to oxidation, hydrolysis, photolysis, and thermal degradation. Degradation products resulting from the stress studies did not interfere with the detection of levofloxacin hemihydrate, and the assay is stability indicating.

show abstract

Section: Introductionmentioning

confidence: 99%

A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form: Application to Pharmaceutical Analysis

Dabhi

Parmar

Patel

et al. 2013

Chromatography Research International

View full text Add to dashboard Cite

show abstract

“…Levofloxacin hemihydrate is official in IP [25]. Numerous HPLC, UV and HILIC⁄MS⁄MS has been used to determine drugs in dosage form and biological fluids [26][27][28][29][30][31][32][33][34].…”

mentioning

confidence: 99%

A Validated Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Cefixime Trihydrate and Levofloxacin Hemihydrate in Pharmaceutical Dosage Form

Patel¹,

Dhananjay²,

Vandana³

et al. 2017

IJAT

View full text Add to dashboard Cite

Cefixime trihydrate (CEF) is an oral third generation cephalosporin class of antibiotic. Chemically, it is (6R, 7R)-7-{[2-(2-amino-1,3-thiazol-4-yl)-2(carboxymethoxyimino)acetyl] amino}-3-ethenyl-8-oxo-5-thia-1 azabicyclo-[4.2.0]oct-2-ene-2 carboxylic acid, clinically used in the treatment of susceptible infections including gonorrhea, otitis media, pharyngitis, lower respiratory-tract infections such as bronchitis, and urinary-tract infections [1,2](Figure 1

show abstract

“…Levofloxacin hemihydrate is official in IP 3 . Numerous HPLC [4][5][6][7][8][9][10] , UV 11 and HILIC⁄MS⁄MS 12 has been used to determine drugs in biological fluids.…”

mentioning

confidence: 99%

Untitled

2014

IJPSR

View full text Add to dashboard Cite

An accurate, precise and reproducible UV-spectrophotometric methods and liquid chromatographic assay method were developed and validated for the determination of Levofloxacin and Ornidazole in tablet dosage form. Spectrophotometric estimation was done by simultaneous equation method and 50% methanol as solvent. In this method λ max for LEVO and OZ were selected at 293.5nm and 318nm. RP-HPLC analysis was carried out using Prontosil C-18 column (4.6 x 250mm, 5μ particle size) and mobile phase composed of Acetonitrile : 0.05% Ortho-phosphoric acid in water pH 3.0 (45:55% v/v)at a flow rate of 1.0 ml/min and chromatogram was recorded at 303 nm. Linearity was evaluated over the concentration range of 4-20 μg/ml and 8-40 μg/mL for LEVO and OZ in both UV spectrophotometric and RP-HPLC method (the value of r 2 = 0.999 found were by both the methods for LEVO and OZ). The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the both methods can be used for routine monitoring of LEVO and OZ in industry in the assay of bulk drug and tablets.

show abstract

Development and validation of RP-HPLC assay for levofloxacin in rat plasma and saliva: Application to pharmacokinetic studies

Cited by 5 publications

References 12 publications

A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form: Application to Pharmaceutical Analysis

A Stability Indicating UPLC Method for the Determination of Levofloxacin Hemihydrate in Pharmaceutical Dosage Form: Application to Pharmaceutical Analysis

A Validated Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Cefixime Trihydrate and Levofloxacin Hemihydrate in Pharmaceutical Dosage Form

Untitled

Contact Info

Product

Resources

About