Development and validation of reverse phase high performance liquid chromatography for citral analysis from essential oils

Gaonkar, Roopa; Yallappa, S; Dhananjaya, Bhadrapura Lakkappa; Hegde, Gurumurthy

doi:10.1016/j.jchromb.2016.10.001

Cited by 17 publications

(14 citation statements)

References 20 publications

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“…However, most studies on plants of the same native species in northeastern Brazil demonstrated the presence of thymol as the main constituent of its EO (MARCHESE et al, 2016). The chemical constituents of CcitEO (lemon grass) were citral 80.7% (45.4 and 35.3% of geranial and neral, respectively) and myrcene (13.4%), a result similar to the concentrations reported in a study using reverse phase high performance liquid chromatography (GAONKAR et al, 2016). The PambEO (Mexican mint) contained thymol (61.5%), p-cymene (11.2%), and γ-terpinene compounds (10.3%) Table 1 -Frequency of the concentration of the major molecular compounds identified in the essential oils of bushy lippia, rosemary pepper, lemon grass, mexican mint and cinnamon using gas chromatography mass spectrometry (GC-MS).…”

Section: Resultssupporting

confidence: 80%

Antimicrobial activity of essential oils from Lippia alba, Lippia sidoides, Cymbopogon citrates, Plectranthus amboinicus, and Cinnamomum zeylanicum against Mycobacterium tuberculosis

2018

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The rise in cases of antibiotic-resistant Mycobacterium tuberculosis has become a major obstacle to the effective control of tuberculosis (TB) worldwide. Essential oils (EO) are complex mixtures that may contain between 20 and 60 components, with two or three major compounds at relatively high concentrations (20-70%) that are responsible for their pharmacological properties. The objective of this study was to assess the antimicrobial activity of the EOs, bushy lippia (Lippia alba), rosemary pepper (Lippia sidoides), lemon grass (Cymbopogon citratus), Mexican mint or Indian borage (Plectranthus amboinicus), and true cinnamon (Cinnamomum zeylanicum), against Mycobacterium tuberculosis H37Rv. Chemical characterization of the EOs was performed by gas chromatography coupled to mass spectrometry. The minimum inhibitory concentration (MIC) was determined by the microdilution-based resazurin microtiter assay. Four EOs were able to inhibit the growth of M. tuberculosis, with MICs of 286.5±130.2μg/mL (C. zeylanicum), 299.5±117.2μg/mL (L. sidoides), 351.6±39.06μg/mL (P. amboinicus), and 1,250μg/mL (C. citratus). Only the EO of L. alba showed no antimycobacterial activity at the tested concentrations, with an MIC greater than 1,250µg/mL. Results of this study suggested that C. zeylanicum, L. sidoides, and P. amboinicus could be important sources of bactericidal compounds against M. tuberculosis and require further investigation. The activity against M. tuberculosis of these three EOs has not been reported previously. The results show the high potential of the tested antimycobacterial EOs, making them a promising alternative for TB treatment. This data also confirms the importance of bioprospecting studies for active substances with antimycobacterial activity, which are still scarce.

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Section: Resultssupporting

confidence: 80%

Antimicrobial activity of essential oils from Lippia alba, Lippia sidoides, Cymbopogon citrates, Plectranthus amboinicus, and Cinnamomum zeylanicum against Mycobacterium tuberculosis

2018

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“…Citral (95%) and geraniol (98%) were obtained from Sigma, India. The essential oil, citral and geraniol were analysed using the newly developed HPLC method in our laboratory and also by GC–MS [ 19 ]. The DMEM medium, FBS, penicillin G, streptomycin were obtained from Invitrogen, India.…”

Section: Methodsmentioning

confidence: 99%

Essential oil from Cymbopogon flexuosus as the potential inhibitor for HSP90

Gaonkar¹,

Shiralgi²,

Lakkappa

et al. 2018

Toxicology Reports

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“…Quantitative determination of MEO, in terms of citral and β-caryophyllene, the two main components of the essential oil, was carried out using the 1200 high performance liquid chromatograph (HPLC) equipped with a diode array detector (DAD), by Agilent Technologies Italia Spa (Rome, Italy). Chromatograms were acquired at 210 nm for β-caryophyllene and 233 nm for citral [22,23]. Chromatographic analyses were performed using a reverse-phase column Eclipse XDB C-18 (150 × 4.6) mm, 3.5 µm particle size, maintained at 27 • C. A gradient elution method, with 0.8 mL/min flow rate, was applied, using (A) acetonitrile and (B) formic acid/water (pH 3.2) as mobile phases.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1

et al. 2020

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Essential oils are complex mixtures of strongly active compounds, very volatile and sensitive to light, oxygen, moisture and temperature. Loading inside nanocarriers can be a strategy to increase their stability and successfully use them in therapy. In the present study, a commercial Melissa officinalis L. (Lamiaceae) essential oil (MEO) was analyzed by gas chromatography-mass spectrometry, loaded inside glycerosomes (MEO-GS) and evaluated for its anti-herpetic activity against HSV type 1. MEO-GS analyses were prepared by the thin layer evaporation method and they were characterized by light scattering techniques, determining average diameter, polydispersity index and ζ-potential. By transmission electron microscopy, MEO-GS appeared as small nano-sized vesicles with a spherical shape. MEO encapsulation efficiency inside glycerosomes, in terms of citral and β-caryophyllene, was found to be ca. 63% and 76% respectively, and MEO release from glycerosomes, performed by dialysis bag method, resulted in less than 10% within 24h. In addition, MEO-GS had high chemical and physical stability during 4 months of storage. Finally, MEO-GS were very active in inhibiting HSV type 1 infection of mammalian cells in vitro, without producing cytotoxic effects. Thus, MEO-GS could be a promising tool in order to provide a suitable anti-herpetic formulation.

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Development and validation of reverse phase high performance liquid chromatography for citral analysis from essential oils

Cited by 17 publications

References 20 publications

Antimicrobial activity of essential oils from Lippia alba, Lippia sidoides, Cymbopogon citrates, Plectranthus amboinicus, and Cinnamomum zeylanicum against Mycobacterium tuberculosis

Antimicrobial activity of essential oils from Lippia alba, Lippia sidoides, Cymbopogon citrates, Plectranthus amboinicus, and Cinnamomum zeylanicum against Mycobacterium tuberculosis

Essential oil from Cymbopogon flexuosus as the potential inhibitor for HSP90

Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1

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