Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1

Vanti, Giulia; Ntallis, Sotirios G; Panagiotidis, Christos Α.; Dourdouni, V; Patsoura, Christina; Bergonzi, Maria Camilla; Lazari, Diamanto; Bilia, Anna Rita

doi:10.3390/molecules25143111

Cited by 20 publications

(21 citation statements)

References 33 publications

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“…Propylene glycol is a colorless, odorless and completely water-soluble solvent very similar to glycerol, also having the GRAS status and high safety [ 30 ]. Glycerosomes were prepared according to the recent publication by Vanti and coworkers [ 5 ]. Different experimental conditions were tested, as reported in the experimental section.…”

Section: Resultsmentioning

confidence: 99%

“…In conclusion, the activity of both pure OOEO and STEO was more prominent when compared to the activity of corresponding amounts of formulated EOs. However, this lower antibacterial and antifungal effectiveness of formulated EOs with respect to pure EOs is mainly due to the prolonged release properties of the EOs loaded in the nanovesicles, as described by other studies on in vitro activity of essential oils formulated in glycerosomes and liposomes [ 5 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…The resulting nanocarrier is stable and efficient, easily and safely produced, capable of stabilizing the essential oil, modulating its release and optimizing its activity [ 4 ]. Recently, a commercial Melissa officinalis L. essential oil was successfully formulated in glycerosomes, being very active in vitro in inhibiting HSV type 1 infection of mammalian cells, without producing cytotoxic effects [ 5 ]. In addition, Risaliti and coworkers (2019) demonstrated enhanced antioxidant, anti-inflammatory and antimicrobial activities of the essential oils of Rosmarinus officinalis L. and Salvia triloba L. plants, when formulated in liposomes [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

et al. 2021

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Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

et al. 2021

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“…During aa optimization procedures, nanoliposome characterization has been performed by evaluating size, homogeneity, and possible aggregation state, with the aid of dynamic and electrophoretic light scattering (DLS and ELS, Zetasizer Nanoseries ZS90) by Malvern Instruments (Worcestershire, UK), by using a scattering angle of 90 °C at 25 °C [ 23 ]. Autocorrelation functions were analyzed by the cumulant method in order to obtain the average hydrodynamic diameter (AHD, nm), the size distribution expressed as PdI (polydispersity index, dimensionless measurement) and ζ-potential (mV), using the software provided by Malvern.…”

Section: Methodsmentioning

confidence: 99%

Co-Delivery of Berberine Chloride and Tariquidar in Nanoliposomes Enhanced Intracellular Berberine Chloride in a Doxorubicin-Resistant K562 Cell Line Due to P-gp Overexpression

et al. 2021

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The MDR phenomenon has become a major obstacle in the treatment of cancers, and among the strategies to reverse it, the inhibition of P-gp function and expression is essential to increase for effective anticancer drugs. In the present paper, the co-delivery of berberine chloride and tariquidar loaded nanoliposomes was investigated with the aim of enhancing solubility and improving desired effects for the antineoplastic drug and the P-gp inhibitor. Developed nanoliposomes were loaded with the electron-dense enzyme horseradish peroxidase, and analyzed by TEM to investigate their ability to enter in both K562 and K562/DOXO cell lines. Receptor-mediated endocytosis was evidenced for both cell lines. Nanoliposomes were loaded with tariquidar, berberine chloride, or both, maintaining chemical and physical characteristics—i.e., size, homogeneity, and encapsulation efficiency—and high suitability for parenteral administration. Tariquidar was able to reverse the MDR in the K562/DOXO cell line. Tariquidar- and berberine chloride-loaded nanoliposomes showed a significant increase of berberine chloride accumulation in tumor cells, which could be correlated with resensitization of the resistant cells to the antitumor agent. These results suggest that the co-delivery of the P-gp inhibitor, tariquidar, and the cytotoxicity inducer, berberine chloride, looks like a promising approach to overcome the MDR.

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“…Essential oils are complex mixtures of strongly active compounds that are very volatile and sensitive to light, oxygen, moisture, and temperature. Loading inside nanocarriers can be a strategy to increase their stability and successfully use them in therapies and in industry [16]. There is significant research showing that essential oils extracted from plants have antibacterial effects.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Biocellulose with Oregano Essential Oil against Cronobacter Strains

et al. 2020

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Biocellulose, named “the biomaterial of the future”, is a natural and ecologically friendly polymer, produced by selected acetic acid bacteria strains. Biocellulose impregnated with antimicrobial agents can be used as a novel, safe, and biodegradable food packaging material, helping extend the shelf life of some products and may also have the chance to replace typical plastic packaging, which is a big environmental problem these days. This study aimed to evaluate if cellulose impregned with natural oregano essential oil could show antibacterial activity against Cronobacter strains, which can occur in food, causing diseases and food poisoning. Bacterial cellulose was obtained from two acetic bacteria strains, Gluconacetobacter hansenii ATCC 23769 and Komagataeibacter sp. GH1. Antibacterial activity was studied by the disc-diffusion method against chosen Cronobacter strains, isolated from the plant matrix. Oregano essential oil has been shown to penetrate into the structure of bacterial cellulose, and after applying cellulose to the solid medium, it showed the ability to migrate. Biopolymer from the strain K. sp. GH1 was able to better absorb and retain essential oregano oil (OEO) compared to bacterial cellulose (BC) produced by the G. hansenii ATCC 23769. Bacterial cellulose with oregano essential oil from strain Komagataeibacter GH1 showed generally greater inhibitory properties for the growth of tested strains than its equivalent obtained from G. hansenii. This was probably due to the arrangement of the polymer fibers and its final thickness. The largest zone of inhibition of strain growth was observed in relation to C. condimenti s37 (32.75 mm ± 2.8). At the same time, the control sample using filter paper showed an inhibition zone of 36.0 mm ± 0.7. A similar inhibition zone (28.33 mm ± 2.6) was observed for the C. malonaticus lv31 strain, while the zone in the control sample was 27.1 mm ± 0.7. Based on this study, it was concluded that bacterial cellulose impregnated with oregano essential oil has strong and moderate antimicrobial activity against all presented strains of the genus Cronobacter isolated from plant matrix. Obtained results give a strong impulse to use this biopolymer as ecological food packaging in the near future.

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Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1

Cited by 20 publications

References 33 publications

Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

Co-Delivery of Berberine Chloride and Tariquidar in Nanoliposomes Enhanced Intracellular Berberine Chloride in a Doxorubicin-Resistant K562 Cell Line Due to P-gp Overexpression

Antibacterial Activity of Biocellulose with Oregano Essential Oil against Cronobacter Strains

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