Development and Validation of Novel Spectro- Chemometric, Chemometric and TLC-Densitometric Methods for Simultaneous Determination of Timolol and Travoprost in their Bulk Powders and Pharmaceutical Formulation

Abstract

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Review on analytical studies of some pharmaceutical compounds containing heterocyclic rings: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol

“…Salem et al [58] established a thin layer chromatography (TLC)-densitometric method for simultaneous determination of a binary mixture of timolol and travoprost without prior separation in their bulk or dosage forms. The separation was carried out on Merck HPTLC aluminum sheets of silica gel 60 F254 using ethyl acetate-methanol-ammonia (5:3:0.5 by volume) as mobile phase.…”

“…The separation was carried out on Merck HPTLC aluminum sheets of silica gel 60 F254 using ethyl acetate-methanol-ammonia (5:3:0.5 by volume) as mobile phase. Detection was done at 274 nm, and linearity was 0.4-50 μg/band for both drugs [58]. Eissa et al [59] suggested a TLC densitometric method for the simultaneous detection of brinzolamide and timolol maleate in pharmaceutical products.…”

“…Brinzolamide and/or timolol maleate Salem et al [58] suggested spectrophotometric and chemometric methods for the simultaneous determination of binary mixture of timolol and travoprost without prior separation in their bulk drugs and dosage forms. The spectrophotometric methods used were derivative spectrophotometry and isosbestic point methods.…”

“…The spectrophotometric methods used were derivative spectrophotometry and isosbestic point methods. Multivariate methods involved the application of three chemometric techniques: classical least square (CLS) principal component regression (PCR), and partial least-squares regression (PLS) [58]. Jadhav et al [66] utilized the spectrophotometric technique for the simultaneous determination of brimonidine tartrate and brinzolamide in bulk and ophthalmic formulation.…”