Development and Validation of Liquid Chromatographic Method for the Simultaneous Estimation of Ezetimibe and Lovastatin in Human Plasma

A viable cost-effective and isocratic approach employing C-18 column (250 mm • 4.6 mm, 5 lm) based HPLC has been utilized to separate and estimate the drug, tranexamic acid in pharmaceutical formulations. Tranexamic acid contains no p-electrons to act as fluorophore or chromophore hence pre-column derivatization was performed with benzene sulfonyl chloride in aqueous medium at room temperature. The derivatized drug was then estimated using C-18 column by exploiting a 25:75 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate (pH 5.0) as the mobile phase. The flow rate of mobile phase was 1 mL/min and detection was performed at a wavelength of 232 nm using UV detector. Retention time of tranexamic acid was 4.42 min. The method followed linear regression equation in the concentration range of 1-100 lg/mL with coefficient of determination equal to 0.9994. The limit of detection and limit of quantitation were 0.3 and 1 lg/mL, respectively. The relative standard deviation and recovery ranges for tranexamic acid were found to be 0.11-2.47% and 97.60-103.25%, respectively. The suggested method is very sensitive and may have the potential to be used for tranexamic acid detection in medicinal formulations.

show abstract

A rapid and sensitive supercritical fluid chromatography/tandem mass spectrometry method for detection of ezetimibein dog plasma and its application in pharmacokinetic studies

Jiang

et al. 2018

Journal of Chromatography B

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Development and Validation of Liquid Chromatographic Method for the Simultaneous Estimation of Ezetimibe and Lovastatin in Human Plasma

Cited by 11 publications

References 21 publications

Simultaneous determination of dexamethasone and moxifloxacin in pharmaceutical formulations using stability indicating HPLC method

Simultaneous determination of dexamethasone and moxifloxacin in pharmaceutical formulations using stability indicating HPLC method

Derivatization/chromophore introduction of tranexamic acid and its HPLC determination in pharmaceutical formulations

A rapid and sensitive supercritical fluid chromatography/tandem mass spectrometry method for detection of ezetimibein dog plasma and its application in pharmacokinetic studies

Contact Info

Product

Resources

About