Development and validation of liquid chromatography–tandem mass spectrometry method for quantification of a potential anticancer triterpene saponin from seeds of Nigella glandulifera in rat plasma: Application to a pharmacokinetic study

Hu, Xiurong; Liu, Xiaobao; Gong, Minghua; Luan, Ming; Zheng, Yang; Wu, Guolan; Shentu, Jianzhong; Zhang, Lin

doi:10.1016/j.jchromb.2014.07.030

Cited by 12 publications

(12 citation statements)

References 15 publications

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“…They reportedly prevent the proliferation of cancer cells [12]. Hu et al [13] identified a compound, Nigella A, which was extracted from Nigella glandulifera (Nepenthaceae) and reported to be the major triterpene saponins. Tannins also exhibit biological activities such as anticancer activity.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity and apoptotic induction of some edible Thai local plant extracts against colon and liver cancer cell lines

Putthawan¹,

Poeaim²,

Areekul³

2018

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 99%

Cytotoxic activity and apoptotic induction of some edible Thai local plant extracts against colon and liver cancer cell lines

Putthawan¹,

Poeaim²,

Areekul³

2018

Trop. J. Pharm Res

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“…The observed C max and AUC of sapindoside B were dosage-dependent in the range of 2.5 to 12.5 mg/kg. Such pharmacokinetic behaviors have been reported in other saponins, including dioscin and nigella A, in the dosage range of 0.0640 to 1.00 mg/kg and 10 to 30 mg/kg after intravenous administration [20,21]. The pharmacokinetic parameters were statistically analyzed by 1-way ANOVA using SPSS software (version 22.0, SPSS Inc.).…”

Section: Resultsmentioning

confidence: 73%

Potential Cytotoxicity, Pharmacokinetics, and Excretion Properties of Sapindoside B from the Seeds of Nigella sativa var. hispidula

Lin

Zhu

et al. 2020

Planta Med

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The seeds of Nigella sativa var. hispidula are widely used in food preparation by the Uighur people in western China. Recently, series of oleanane triterpenoid saponins were extracted from the seeds of Nigella sativa var. hispidula, especially α-hederin as representative that exhibited strong antitumor activity. Compared to α-hederin, sapindoside B has just 1 more terminal xylopyranose in the 3-O position and displays similar effects against various human cancer cell lines with cisplatin. Considering this potential cytotoxic activity, a reliable LC-MS/MS method was developed to quantify sapindoside B in rat plasma, urine, and feces. Chromatographic separation was conducted on an Agilent Zorbax SB-Aq (3.0 × 150 mm, 3.5 µm) column via an isocratic elution procedure with acetonitrile and water containing 0.1% formic acid. Mass spectrometric detection was coupled with an electrospray ionization source in the MRM mode. The linear range of calibration curves was 15 ~ 3000 ng/mL in plasma/urine and 30 ~ 3000 ng/g in feces. The intra-day and inter-day precision was less than 11.1%, and accuracy ranged from 92.2% to 108.7%. The proposed method was validated and shown to be reliable, precise, and accurate and was successfully applied to its pharmacokinetics and excretion studies. Sapindoside B exhibited dosage-dependent pharmacokinetics in the range of 2.5 mg/kg to 12.5 mg/kg, and only about 2% of intravenous dose of sapindoside B was excreted by the feces and urine in its unchanged form over 48 h. These results provide further data support for evaluating the druggability of sapindoside B.

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“…For example, N. glandulifera and TSN were con rmed to exhibit signi cant antioxidant and anti-in ammatory properties (Zhao et al 2013;Zheng et al 2020). Triterpene saponins are the dominant group of anti-in ammatory compounds existed in this ethnomedicinal plant (Hu et al 2014;Hu et al 2020) and Nigella A was a major ingredient of triterpene saponins extracted from N. glandulifera. Nigella A was 60.36 ± 1.25 g/100 g total saponins of N. glandulifera (Zhao et al 2013).…”

Section: Discussionmentioning

confidence: 97%

“…Similar to our previous research (Zhang et al 2020), DSS was bought from MP Biomedicals (MP Biomedicals, USA). Nigella A was isolated and puri ed from N. glandulifera in our lab with the purity of 98.4% (Hu et al 2014), and its structure was con rmed by MS, 1H and 13C NMR spectra.…”

Section: Drugs and Reagentsmentioning

confidence: 99%

“…Although the anti-in ammatory activity of N. glandulifera and its main constitutes have gained a great deal of attention in recent years, no research has been conducted on its anti-colitis activity. In our previous study, a large amount of Nigella A was extracted and puri ed from the seeds of N. glandulifera (Hu et al 2014;Hu et al 2020). Due to these healthcare functions, it is worthwhile to explore the therapeutical effect of Nigella A, a main active triterpene saponin extracted from N. glandulifera, on UC.…”

Section: Introductionmentioning

confidence: 99%

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Nigella A ameliorates experimental colitis via interacting with gut microbiota on dextran sulphate sodium (DSS)-induced C57BL/6 mice

Xu¹,

Yang²,

Zheng³

et al. 2020

Preprint

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Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and SASP could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.

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Development and validation of liquid chromatography–tandem mass spectrometry method for quantification of a potential anticancer triterpene saponin from seeds of Nigella glandulifera in rat plasma: Application to a pharmacokinetic study

Cited by 12 publications

References 15 publications

Cytotoxic activity and apoptotic induction of some edible Thai local plant extracts against colon and liver cancer cell lines

Cytotoxic activity and apoptotic induction of some edible Thai local plant extracts against colon and liver cancer cell lines

Potential Cytotoxicity, Pharmacokinetics, and Excretion Properties of Sapindoside B from the Seeds of Nigella sativa var. hispidula

Nigella A ameliorates experimental colitis via interacting with gut microbiota on dextran sulphate sodium (DSS)-induced C57BL/6 mice

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