Development and validation of HPTLC method for estimation of gymnemic acid in microencapsulated antidiabetic polyherbal formulations

Abstract

Summary. Gymnemic acid (GA) is one of the phytoconstituents present in Gymnema sylvestre. Estimation of GA was carried out first time from microencapsulated polyherbal formulation. Microencapsulated polyherbal formulations (F1 and F2) contain various plant extracts; hence, proper resolution of GA peak in high-performance thin-layer liquid chromatography (HPTLC) analysis of F1 and F2 is the problem. Hence, HPTLC analysis method for F1 and F2 is developed and validated for quantitative determination of GA. HPTLC analysis of F1 and F2 was carried out using TLC aluminum plates precoated with silica gel 60F 254 eluted with chloroform-methanol-water (6.5 mL + 4.5 mL + 1.0 mL), and densitometric analysis was carried out at 580 nm. Complete validation was performed using standard methods. This HPTLC method was found to be reproducible, accurate, and can detect GA at microgram level. The new optimized mobile phase gave good resolution of GA peak for its proper quantification in microencapsulated polyherbal formulation.

Determination of gymnemic acid level in Gymnema inodorum leaves using multiple reaction monitoring mass spectrometry

“…Since the derivatives of GA are comprised of the GM skeleton, the quantity of GM is equivalent to that of GA. The detection of GM can be carried out by using thin layer chromatography [9,10], high performance liquid chromatography with UV or mass detectors [11 -13]. In addition, Kamble et al [14] showed that the method of HPLC-MS electrospray ionization with multiple reaction monitoring was a high throughput technique for measuring GM aglycone of total GA derivatives in G. sylvestre extract and commercial products.…”

Binding of gymnemic acid II to mammalian glycerol-3-phosphate dehydrogenase

“…G. sylvestre extracts contain triterpene saponins belonging to the oleanane and dammarane classes [1]. GAs, a complex mixture of oleanane saponins, are generally considered the active constituents, and the quality of the extracts and their formulations is assessed by the content of GAs [12]. Although several studies have reported the pharmacological effects of crude GA extracts, few studies on the effects of a single molecule have been reported.…”