Development and validation of HPTLC method for simultaneous determination of quercetin and kaempferol in leaves of two chemotypes of<i>Centella asiatica</i>

Joshi, Chaitali; Savai, Jay; Varghese, Alice; Pandita, Nancy

doi:10.1556/jpc.25.2012.5.8

Cited by 8 publications

(9 citation statements)

References 6 publications

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“…13 It is hydrophobic in nature and freely soluble in methanol, 1, 4 -dioxane, Ethanol and dimethylformamide. 14,15 In a wide range of HPLC and HPTLC techniques were reported for estimation of kaempferol in extracts, [16][17][18][19][20] gingko biloba tablets, 21,22 phytosome formulation 23 (Kaempferolphospholipids complex) at around 360 nm. Literature survey revealed that no simple UV -visible method has Original Article been reported for estimation of kaempferol in phytosome formulations.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of UV Spectrophotometric Method for the Estimation of Kaempferol in Kaempferol: Hydrogenated Soy PhosphatidylCholine (HSPC) Complex

Telange¹,

Patil²,

Tatode³

et al. 2014

Phm

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Introduction: Kaempferol (3, 5, 7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) is a natural fl avonoid belongs to a subcategory of fl avonol family. The kaempferol -hydrogenated soy phosphatidylcholine (HSPC) complex was obtained by refl uxing and freeze drying method. Ultra violet (UV) -visible spectrophotometric method has been developed for the determination of kaempferol in kaempferol -HSPC complex. Objective: A validated UV -visible spectrophotometric method for determination of kaempferol in Kaempferol -HSPC complex. Materials and Methods: The Kaempferol -HSPC complex (Phytosomes) were prepared by dissolving both kaempferol and HSPC in 1, 4 -dioxane for refl uxing up to 2 h and freeze dried. The spectrophotometric detection of kaempferol was done at absorption maximum (λ max ) of 365 nm and 265 nm using methanol as solvent. The developed method was validated as per ICH guidelines. Result: The kaempferol content in Kaempferol -HSPC complex was found to be 79.32% and 79.19% at 365 nm and 265 nm. Kaempferol demonstrated good linearity in concentration range of 2-12 μg/ml (r 2 > 0.99) at 365 nm and 2-14 μg/ml (r 2 > 0.99) at 265 nm. Precision and mean recoveries were found to be in the range of (%RSD 0.0957 and 0.0580) and (% RSD 0.1461 and 0.0959) and 99.70% and 91.85% at 365 nm and at 265 nm. Limit of detection and limit of quantifi cation were found to be (0.015 μg/ml and 0.0191 μg/ml) and (0.0457 μg/ml and 0.0579 μg/ml) respectively. Conclusion: The developed method was found to be simple, specifi c, economic, reliable, accurate, precise, reproducible and used as a quality control tool for analysis of Kaempferol.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of UV Spectrophotometric Method for the Estimation of Kaempferol in Kaempferol: Hydrogenated Soy PhosphatidylCholine (HSPC) Complex

Telange¹,

Patil²,

Tatode³

et al. 2014

Phm

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“…This indicated that the method was accurate. Simutaneous quantification of kaempferol and quercetin in plant extracts: Though quantification of kaempferol and quercetin by TLC densitometric method was reported for other plants 14,15,16 , this is the first report presenting that kaempferol and quercetin were simultaneously quantified in extracts of Podophyllum and Senna. Validated TLC method was used for quantifying and resolving kaempferol and quercetin in the extract.…”

Section: Methods Validationmentioning

confidence: 90%

Untitled

2016

IJPSR

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Kaempferol and quercetin are the flavonoids which are widespread in fruits and vegetables exhibiting important biological activities. A simple and precise HPTLC method was developed and validated for simultaneous determination of kaempferol and quercetin in herbal drug extracts of Podphyllum hexandrum and Cassia angustifolia. The HPTLC of flavonoids was performed with toluene: acetone: formic acid (7:3:0.25) as mobile phases on RP-18 F254 TLC plates. Densitometric determination of flavonoids was performed at λ = 254 nm in reflectance/ absorbance mode. The results obtained indicated that the developed method was precise and accurate. Good resolution of kaempferol and quercetin was obtained from other constituents present in methanolic and hydrolysed extracts of P. hexandrum and C. angustifolia using proposed HPTLC method. Kaempferol was quantified in free (8.01and 2.42 µg/mg) as well as bound (17.54 and 8.13 µg/mg) forms and quercetin in free (4.5 and 0.93 µg/mg) as well as bound form (6.17and 4.8 µg/mg) in P. Hexandrum and C. angustifolia. Kaempferol was found to be present in high concentration and quercetin was in low concentration in these plants. Statistical data showed that method is sensitive and selective for simultaneous determination of flavonoids.

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“…CA-11 (Table 1). There have been several reports on the profiling of secondary metabolites from the samples of C. asiatica collected from different locations, which involved phytochemical analysis of all four major triterpenoids (Randriamampionona et al , 2007), minor triterpenoids (Zhang et al , 2009), glycosides (Thomas et al , 2010), aglycones (Joshi et al , 2012) or selected glycoside and its corresponding aglycones (Devkota et al , 2010). An extensive report on 60 different accessions of C. asiatica has been based on HPTLC analysis, wherein only triterpenoid glycosides have been profiled (Thomas et al , 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Owing to the diverse medicinal uses of C. asiatica , qualitative as well as quantitative studies are conducted to evaluate phytochemical content of the plant. To assess natural and genetic variability, a comparative quantitative analysis of phytochemicals in the selected accessions of C. asiatica collected from different locations is reported (Thomas et al , 2010; Zhang et al , 2009; Joshi et al , 2012). Studies have also assessed the levels of asiaticoside and madecassoside in various parts of the plant with different phenotypes being observed in vitro and in vivo (Aziz et al , 2007; Singh et al , 2014).…”

Section: Introductionmentioning

confidence: 99%

Profiling of triterpenoid saponin content variation in different chemotypic accessions of Centella asiatica L.

Singh

Sangwan

Gupta

et al. 2014

Plant Genet. Resour.

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Centella asiatica (L.) Urban is an important herbaceous medicinal plant with a worldwide distribution. The herb possesses a medicinal value and is used extensively in traditional systems of medicine. The medicinal properties of the herb are attributed to the presence of characteristic triterpenoids and their saponins in the leaves. The major triterpenoids are asiaticoside, madecassoside and their aglycones asiatic acid and madecassic acid, respectively, among others. The present study reports a remarkable qualitative and quantitative variability in secondary metabolites in different accessions of C. asiatica L. as determined by high-performance liquid chromatography (HPLC) analysis. The accessions analyzed in this study can be considered as the core set of discrete chemotypes of C. asiatica. Considerable and contrasting biochemical variations were observed in the terpenoid profiles of the chemotypes. From the basic and applied phytochemical utility, this chemotypic variability in the total content of triterpenoids is important and interesting.

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Development and validation of HPTLC method for simultaneous determination of quercetin and kaempferol in leaves of two chemotypes ofCentella asiatica

Cited by 8 publications

References 6 publications

Development and Validation of UV Spectrophotometric Method for the Estimation of Kaempferol in Kaempferol: Hydrogenated Soy PhosphatidylCholine (HSPC) Complex

Development and Validation of UV Spectrophotometric Method for the Estimation of Kaempferol in Kaempferol: Hydrogenated Soy PhosphatidylCholine (HSPC) Complex

Untitled

Profiling of triterpenoid saponin content variation in different chemotypic accessions of Centella asiatica L.

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