Development and Validation of UV Spectrophotometric Method for the Estimation of Kaempferol in Kaempferol: Hydrogenated Soy PhosphatidylCholine (HSPC) Complex

Abstract

Introduction: Kaempferol (3, 5, 7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) is a natural fl avonoid belongs to a subcategory of fl avonol family. The kaempferol -hydrogenated soy phosphatidylcholine (HSPC) complex was obtained by refl uxing and freeze drying method. Ultra violet (UV) -visible spectrophotometric method has been developed for the determination of kaempferol in kaempferol -HSPC complex. Objective: A validated UV -visible spectrophotometric method for determination of kaempferol in Kaempferol -HSPC complex. Materials and Methods: The Kaempferol -HSPC complex (Phytosomes) were prepared by dissolving both kaempferol and HSPC in 1, 4 -dioxane for refl uxing up to 2 h and freeze dried. The spectrophotometric detection of kaempferol was done at absorption maximum (λ max ) of 365 nm and 265 nm using methanol as solvent. The developed method was validated as per ICH guidelines. Result: The kaempferol content in Kaempferol -HSPC complex was found to be 79.32% and 79.19% at 365 nm and 265 nm. Kaempferol demonstrated good linearity in concentration range of 2-12 μg/ml (r 2 > 0.99) at 365 nm and 2-14 μg/ml (r 2 > 0.99) at 265 nm. Precision and mean recoveries were found to be in the range of (%RSD 0.0957 and 0.0580) and (% RSD 0.1461 and 0.0959) and 99.70% and 91.85% at 365 nm and at 265 nm. Limit of detection and limit of quantifi cation were found to be (0.015 μg/ml and 0.0191 μg/ml) and (0.0457 μg/ml and 0.0579 μg/ml) respectively. Conclusion: The developed method was found to be simple, specifi c, economic, reliable, accurate, precise, reproducible and used as a quality control tool for analysis of Kaempferol.

Biosynthesized Highly Stable Au/C Nanodots: Ideal Probes for the Selective and Sensitive Detection of Hg2+ Ions

“…The spectrum shows five bands at 265, 293, 320, 342, and 377 nm, which is attributed to characteristic peaks of biomolecules like anthocyanins, quercetin, and kaempferol etc., which are present in the extract and can act as a reducing agent as well as a carbon precursor in the formation of GCNDs. The results are well matched with reported literature [ 46 , 47 , 48 ]. Figure 5 b,c show the UV-Vis absorbance and PL spectra of the synthesized GCNDs.…”

Anti-Oxidative Activity of Alcohol-Water Extracts from Field Horsetail (Equisteum arvense) in Elastomer Vulcanizates Subjected to Accelerated Aging Processes

“…nm and 300-380 nm are characteristic of other active compounds present in field horsetail, such as flavonoids (kaempferol, quercetin, rutin and others) and related compounds[45][46][47]. Therefore, absorption peaks in the range of 350-220 nm found in the spectra of hydroalcoholic extracts confirm the presence of a mixture of active phenolic acids, flavonoids and related compounds contained in the samples.…”

“…According to the research on phenolic compounds, natural phenolic acids such as vanillic acid, gallic acid, caffeic acid and ferulic acid show strong absorption bands in the range of 270-320 nm [6,44]. Moreover, the absorption bands at 240-280 nm and 300-380 nm are characteristic of other active compounds present in field horsetail, such as flavonoids (kaempferol, quercetin, rutin and others) and related compounds [45][46][47]. Therefore, absorption peaks in the range of 350-220 nm found in the spectra of hydroalcoholic extracts confirm the presence of a mixture of active phenolic acids, flavonoids and related compounds contained in the samples.…”

Onionskin waste versus synthetic additives in broiler diet: influence on production indices, oxidative status, caecal bacteria, immune indices, blood chemistry and meat quality

“…Quecetin in OW was determined spectrophotometrically as described by Pejic et al (2004). Kaempferol in OW was determined by a spectrophotometric method described by Telange et al (2014).…”