Development and Validation of HPLC Method for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Bulk and Pharmaceutical Dosage Form

Abstract

Brimonidine tartrate and Timolol Maleate are used in treatment of glaucoma by decreasing intra ocular pressure. A validated HPLC method was developed for the assay of them. The method was performed on BDS HYPERSIL Cyano column (250×4.6 mm, 5 μ) and the mobile phase consisted of Ammonium acetate (pH 5.0, 0.01 M) -Methanol (40:60, V/V) which pumped at a flow rate equals to 1.5 ml/min at ambient temperature. 20 µl of drugs sample solutions were monitored at two fixed wavelengths (lambda=254.0 nm for Brimonidine Tartrate and 300.0 nm for Timolol Maleate). The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation according to ICH.

Fast separation and quantification of three anti-glaucoma drugs by high-performance liquid chromatography UV detection

“…In comparison with the reported methods for TM/BM mixture, the proposed method was 2 times more sensitive for TM and 5, 2 and 2.5 times more sensitive for BM than the reported HPLC methods [ 30 , 31 , 33 , respectively]. While the proposed method was 100 times more sensitive for both TM and BM than the reported HPTLC method [ 35 ].…”

“…Some HPLC methods [ 36 – 38 ] are also reported for the determination of TM/LP mixtures. These methods have some weaknesses such as poor sensitivity [ 30 , 31 , 33 – 37 ], narrow linearity ranges [ 30 , 31 , 33 – 35 , 38 ], poor column efficiency [ 32 ], and need for time-programmed UV detection [ 32 , 37 , 38 ], column-temperature control [ 37 ], or gradient elution [ 38 ].…”

Review on analytical studies of some pharmaceutical compounds containing heterocyclic rings: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol

“…Chromatographic analysis Brinzolamide and/or timolol maleate Surveying literature 2000-2019 revealed that many chromatographic analysis methods have been applied for the determination of brinzolamide as well as timolol maleate. HPLC is the most commonly applied chromatographic technique for the determination of brinzolamide and timolol maleate [44][45][46][47][48][49][50][51][52][53][54][55][56][57].…”

Ion-Selective Membrane Sensors for Determination of Brimonidine Tartrate in Bulk Powder, Pharmaceutical Eyedrops and Biological Fluids.

“…The literature survey reveals several analytical methods for quantitative estimation of BRD in body fluids and in pharmaceutical formulations by spectrophotometry [4][5][6][7][8][9][10], liquid chromatography [11][12][13][14][15][16][17][18][19][20][21] and electrochemical methods [22][23][24].…”