Development and validation of HPLC-UV method for the quantitative analysis of carcinogenic organic impurities and its isomers in the sodium polystyrene sulfonate polymer

Abstract

<jats:title>Abstract</jats:title> <jats:p>Sodium polystyrene sulfonate (SPS) powder is in use for over 50 years for the treatment of hyperkalemia. SPS powder is official in United States Pharmacopoeia, British Pharmacopoeia and European Pharmacopoeia. However, till date, no study has been published on the assessment of organic impurities for this drug. The organic impurities in bulk drug and finished product are associated with their safety, efficacy and stability. A simple, rapid, specific, precise and an accurate HPLC method has been developed for the estimation of toxic organic impurities like styrene, naphthalene, divinyl benzene (DVB) and ethylvinyl benzene (EVB) from SPS bulk drug and finished product. The developed method was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantitation (LOQ), solution stability, ruggedness and robustness. The influence of acid, alkali, oxidative stress, photolytic stress, thermal stress and humidity stress conditions on SPS bulk powder and finished product has been studied and reported. The proposed method can be successfully employed for the impurity testing of commercial batches of the bulk drug and finished products of both sodium salt and calcium salt of polystyrene sulfonate.</jats:p>

Estimation of Nicotine in Tobacco Extract Using C8 Column in High Performance Liquid Chromatography

“…There are several methods of nicotine estimation including gas chromatography with flame ionization detector (GC-FID), 10 and gas chromatography-mass spectrometry (GC-MS). 11,12 Since, LC is a workhorse technique used for efficient and tedious analytical procedures, [13][14][15] it has been employed successfully for nicotine quantification in e-liquids such as liquid chromatography-mass spectrometry (LC-MS), 16,17 in addition to HPLC with photodiode array detection (HPLC-PDA). 18,19 HPLC method is the rapid, simple and efficient of all.…”

Simple HPLC method for rapid quantification of nicotine content in e-cigarettes liquids

“…Nicotine concentration has been determined by several analytical techniques including gas chromatography with flame ionization detector (GC-FID) [9], and gas chromatography-mass spectrometry (GC-MS) [8,10]. Since LC is a workhorse technique used for efficient and tedious analytical procedures [19][20][21]. It has been employed successfully for nicotine quantification in e-liquids such as liquid chromatography-mass spectrometry (LC-MS) [6,7], in addition to HPLC with photodiode array detection (HPLC-PDA) [13,14].…”

Molecular Insight into the Effects of Clustering on the Dynamics of Ionomers in Solutions

“…Ionizable groups tethered to polymers enable ion transport that in turn propels innovative lightweight energy technologies , as well as groundbreaking biomedical applications. , The ionizable groups drive the formation of ionic clusters, determining the structure and dynamics of the polymers and, subsequently, their ability to transport ions. Most notable is the immense impact even a small number of ionizable groups tethered to the polymer have on constraining the motion of the entire chain, as has been shown by rheology. − This restricted motion of the chains often results in far-from-equilibrium trapped states, as macromolecules are processed into viable materials that present a challenge to the formation of well-defined reproducible structures with desired properties.…”

“…I onizable groups tethered to polymers enable ion transport that in turn propels innovative lightweight energy technologies 1,2 as well as groundbreaking biomedical applications. 3,4 The ionizable groups drive the formation of ionic clusters, determining the structure and dynamics of the polymers and, subsequently, their ability to transport ions. Most notable is the immense impact even a small number of ionizable groups tethered to the polymer have on constraining the motion of the entire chain, as has been shown by rheology.…”