Development and Validation of HPLC Method for the Simultaneous Determination of Enalapril Maleate in Present of Their Impurities: Application to Tablet Analysis

Logoyda, Liliya; Abdel‐Megied, Ahmed M.; Kondratova, Yuliya; Trofimenko, Olena; Кorobko, Dmytro; Dakhym, I. S.

doi:10.22159/ijap.2018v10i1.22805

Cited by 28 publications

(17 citation statements)

References 12 publications

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“…In the present study, optimization and critical evaluation of mobile phase composition (gradient), flow rate, and analytical column were important to obtain good resolution of peaks of interest from the endogenous components, which, in turn, affect reproducibility and sensitivity of the method [4][5][6]. The resolution of peaks was best achieved with Discovery C18, 50×2.…”

Section: Resultsmentioning

confidence: 98%

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Logoyda

2018

Asian J Pharm Clin Res

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Objective: A simple, rapid high-performance liquid chromatography-mass spectrometry/mass spectrometry (MS/MS) method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. . The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 ml/min into the MS electrospray ionization chamber. The sample volume was 5 µl.Results: Under these conditions, amlodipine was eluted at 1.61 min. According to the Caco-2 test results, amlodipine appeared to have moderate to high permeability. Recovery value for amlodipine is 52.33%. Caco-2 permeability values for amlodipine are in agreement with BCS Class I classification for these drugs and their known high bioavailability in humans. Conclusion:A new rapid method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for the examination of amlodipine from Caco-2 cell monolayers.

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Section: Resultsmentioning

confidence: 98%

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Logoyda

2018

Asian J Pharm Clin Res

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“…In the present study, optimization and critical evaluation of mobile phase composition, flow rate, and analytical column were important to obtain good resolution of peaks of interest from the endogenous components, which, in turn, affect reproducibility and sensitivity of the method [7][8][9][10][11][12][13]. The resolution of peaks was best achieved with Discovery C18, 50×2.1 mm, and 5 μm column.…”

Section: Resultsmentioning

confidence: 99%

A High-Performance Liquid Chromatography-Mass Spectrometry Method Development for the Quantitative Determination of Enalapril Maleate From Caco-2 Cell Monolayers

Logoyda

2018

Asian J Pharm Clin Res

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Objective: A simple, rapid high-performance liquid chromatography-mass spectrometry (HPLC MS/MS) method was developed for the determination of enalapril maleate from confluent Caco-2 monolayers and aqueous solution.Methods: Chromatography was achieved on Discovery C18, 50×2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A [acetonitrile-water-formic acid, 5: 95:0.1 v/v] and eluent B [acetonitrile-formic acid, 100:0.1 v/v]). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.4 ml/min into the mass spectrometer ESI chamber. The sample volume was 5 μl.Results: Under these conditions, enalapril maleate was eluted at 1.52 min. According to the Caco-2 test results, enalapril showed low permeability. It should be noted that the recovery value for enalapril is 100.62 %. Low in vitro permeability data for enalapril are in good agreement with the literature data.Conclusion: From results of the analysis, it can be concluded that developed method is simple and rapid for determination of enalapril maleate from confluent Caco-2 monolayers and aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for examination of enalapril from Caco-2 cell monolayers.

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“…Our objеctivе of the chromatоgraphіc method develоpment wаs to achiеve a peak tailing factor <1.5, retention time in between 1 and 3 min, along with gооd resоlution. [9][10][11] Ascentis express columns, based on Fusеd-core pаrticle technоlogy, prоvide more than twice the speed and efficiency of traditiоnal cоlumns at half the backpressure of sub-2-µm columns. This objеctive was obtainеd using mobile phase consisting of methanol and 0.1% solution of trifluoroacetic acid (40/60, v/v).…”

Section: Discussionmentioning

confidence: 99%

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Logoyda¹

2018

AJP

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Introduction:In recent years, the principle and theory behind solvent extraction has gained wide acceptance as one of the new tools of modern pharmaceutical analysis. Analytical chemists have been using solvent extraction in the laboratory for many years for extracting molecular species from aqueous solutions by organic solvents. However, the requirements and objectives of an analytical procedure are quite different from those of a profit producing mill flow sheet. Depending on the nature, aggregate state, sample concentration and method of analysis, and various sampling procedures are used: Moisture removal, grinding, decomposition, dissolution, melting, elution, removal of the matrix, dilution, concentration, etc. The objective of this research was to select the optimal conditions for the extraction for captopril by organic solvents from water solutions in dependence on pH solutions. Materials and Methods: The chromatographic analysis of captopril performed on liquid chromatography ACQUITY Arc system. Results: The obtained data testify that the optimal solvents for the extraction of captopril in the process of separating it from objects of biological origin are chloroform and methylene chloride. The amount of substance released by chloroform is 91.48% (at pH 1), methylene chloride is 97.97% (at pH 1), and hexane is 69.65% (at pH 2). Conclusion: The extraction of captopril by organic solvents from water solutions in dependence on pH solutions has been conducted. As a result of studies, we have found that the optimal extragent is methylene chloride, which is extracted at pH 1-97.97% and chloroform is extracted at pH 1-91.48%.

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Development and Validation of HPLC Method for the Simultaneous Determination of Enalapril Maleate in Present of Their Impurities: Application to Tablet Analysis

Cited by 28 publications

References 12 publications

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

A High-Performance Liquid Chromatography-Mass Spectrometry Method Development for the Quantitative Determination of Enalapril Maleate From Caco-2 Cell Monolayers

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