A High-Performance Liquid Chromatography-Mass Spectrometry Method Development for the Quantitative Determination of Enalapril Maleate From Caco-2 Cell Monolayers

Logoyda, Liliya

doi:10.22159/ajpcr.2018.v11i7.25455

Cited by 3 publications

(7 citation statements)

References 16 publications

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“…Approaching the standardization of liquid and dense thyme creeping extract, we considered the possibility of identifying the same BAS, continuing the proposed approach in the HMP chain extracts [9]. The determination of the amount of flavonoids was carried out by the method of differential spectrophotometry on the spectrophotometer of the brand "Cary-50" [12]. The quantitative content of flavonoids in the dense extract was determined by the reaction of the formation of the colored compound directly of flavonoids with aluminum chloride.…”

Section: Methodsmentioning

confidence: 99%

“…With a vacuum dilution of 0.8 kp/cm 2 , the gain is about 15-20% in comparison with the vacuum of 0.6 kp/cm 2 Identification and determination of the quantitative content of the analyzed BAS in HMP and their respective extracts were carried out using modern sensitive and selective methods of analysis. The qualitative composition of flavonoids was determined by the thinlayer chromatography method [11,12] in the solvent system ethyl acetate Pformic acid Pwater P (90:6:9). The determined volumes of solutions were applied to chromatographic plates Silica gel F254 of company "Merck" using a device for automatic sampling on "CAMAG Linomat 5" plate, chromatography was carried out in a "GAMAG" chromatography chamber.…”

Section: Methodsmentioning

confidence: 99%

“…The determination of the amount of flavonoids was carried out by the method of differential spectrophotometry on the spectrophotometer of the brand "Cary-50" [12].…”

Section: Methodsmentioning

confidence: 99%

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Development of Methods of Analysis of Biological Active Substances for Standardization of Dense Extract of Creeping Thyme

2020

Lett Appl NanoBioSci

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Pharmaceutical development of mucolytic agent with dense extract of creeping thyme and essential oil of thyme involves obtaining a dense thyme extract of creeping, that is, choosing of optimal conditions that will allow in a short time to obtain a dense extract enriched with the main biological active substances (BAS) - which is a resultant extract and finished drug. Since in the analysis of creeping thyme herb in order to standardize this raw material as a quality index, among others, we chose the composition of flavonoids and hydroxycinnamic acids and the quantitative content of flavonoids, then it is appropriate to study the qualitative and quantitative composition of these bioactive substances and in the obtained extract. The aim of our work was to develop methods of analysis of BAS for the standardization of dense extract of creeping thyme; Identification and determination of the quantitative content of the analyzed biological active substances in herbal medicinal products and their respective extracts were carried out using modern sensitive and selective methods of analysis (thin-layer chromatography, differential spectrophotometry); As a result of TLC, the presence of fructose, glucose, arabinose, xylose, and rhamnose were found in the dense creeping thyme extract. According to the ratio of the size and intensity of staining on the chromatograms, it has been concluded that the predominant monosaccharide is fructose, and from the reducing monosaccharides - glucose, which will allow them to choose markers of the quality of the test extract. We also identified tyrosine, alanine, leucine, glycine, glutamic and aspartic acids at the levels of the respective zones on the chromatogram of the comparison solution. Chromatographic studies of a dense extract of creeping thyme by the thin-layer chromatography method have allowed identifying phenol carboxylic acids – rosmarinic (main representative), caffeic and chlorogenic; flavonoids – luteolin-7-O-glucoside, apigenin-7-O-glucoside, luteolin, apigenin, and rutin. Two acids were not identified, small quantities of which are found in the upper part of a chromatogram and of the unidentified structure of luteolin glucoside, which, by size and intensity of fluorescence, are not the main representative of flavonoids in the extract. Based on the literature, this representative of flavonoids may be Luteolin-7-O-di or triglycoside; Qualitative composition of a dense extract of creeping thyme herb in BAS groups – flavonoids, amino acids, polysaccharides has been studied. In order to standardize the extract, identification markers from phenolic compounds – luteolin-7-O-glucoside, luteolin, rutin, chlorogenic, rosmarinic, and caffeic acids, luteolin glycoside of unknown composition has been proposed. The quantitative content of flavonoids, amino acids, and reconstructive monosaccharides in the dense extract has been investigated. The correlation of content of the main BAS groups for dense extract indicates that the selected conditions for transferring the liquid extract to the dense are correct. When standardizing the dense extract of creeping thyme by the flavonoid content is proposed to be at least 2.5% in terms of apigenin.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Development of Methods of Analysis of Biological Active Substances for Standardization of Dense Extract of Creeping Thyme

2020

Lett Appl NanoBioSci

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“…Both evaluated drugs were "rapidly dissolving" [ Table 1] because the active pharmaceutical ingredient release at time point 30 min was >85%. [21][22][23][24][25] Table 1.…”

Section: If Both the Test And The Reference Dosage Forms Are Verymentioning

confidence: 99%

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Logoyda¹

2019

AJP

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Introduction: Biowaiver procedure, nowadays, is a trend of Modern Biopharmaceuticals. The biowaiver approach based on Biopharmaceutical Classification System BCS is intended to replace bioequivalence in vivo studies. Bisoprolol is a medicine most commonly used for heart diseases, the beta blocker family of medications, and is of the β 1-selective type. The Aim of the Study: The aim was to study the dissolution kinetics of bisoprolol tablets to assess their equivalence under conditions in vitro according to the biowaiver. Methods: The study of dissolution kinetics of drugs in the form of bisoprolol tablets has been carried out in accordance with the requirements of the "biowaiver" procedure, the recommendations of the SPhU, and the WHO requirements to assess the possibility of replacing the pharmacokinetic studies in vivo by tests in vitro. Results and Discussion: The possibility to use the recommendations of the "biowaiver" procedure for the registration of generics bisoprolol tablets has been found. The studies conducted have shown that bisoprolol can be referred to Class І of the BCS, i.e., substances with a high biopharmaceutical solubility and a high penetration rate. It will allow conducting comparative studies in vitro to confirm the equivalence of drugs. Conclusions: The evaluated bisoprolol drugs fulfill biowaiver criteria for drugs containing BCS Class I active pharmaceutical ingredients. Both drugs are "rapidly dissolving," both meet the criteria of dissolution profile similarity, f 2 (i.e., the dissolution profile of the test product is similar to that of the reference product in pH 1.2, 4.5, and 6.8 buffers using the paddle method at 75 rpm), and both are considered to be in vitro equivalent without in vivo evaluation.

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“…For a successful study of this substance by these methods, usually, derivatization of active groups with one or more reagents is carried out. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] However, as practice shows, in case of using tianeptine for the purpose of obtaining a narcotic effect, urine contains products of its metabolism or disintegration.…”

Section: Original Articlementioning

confidence: 99%

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Logoyda¹

2019

IJGP

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Introduction: Coaxil (tianeptine) is a new generation antidepressant with selective serotoninergic action. Recently, a significant increase in non-medical use of the atypical tricyclic antidepressant drug, tianeptine, has been observed in Ukraine. According to domestic narcologists and psychiatrists, there is information on tianeptine abuse by drug addicts. In literature, there is information that this medicine combines characteristics of opium, heroin, and cocaine in it. Receiving of tianeptine in high dosages (400-600 mg intravenously) leads to a state of euphoria and gives a narcotic effect that is several times stronger than heroin and cocaine. The aim of the Study: The aim of the research was to study of tianeptine metabolism products in the human body in urine by gas chromatography-mass spectrometry. Methods: The analysis has been carried out on a gas chromatograph Agilent 6890N with a massselective detector Agilent 5973 and an injector Agilent 7683 (USA) equipped with a chromatographic quartz capillary geyser of FactorFour Varian (USA). Gas carrier was helium. Results and Discussion: The analysis of extracts received as with acidic and with alkaline pH values has shown metabolites of tianeptine MC5 in them. Besides the main metabolite (MC5), there have also been desalkyldesaminotianeptine and desalkyldesaminonortianeptine in the extracts obtained at pH 3-4 and in the extracts obtained at pH 9-10-desalkyltianeptine and a metabolite, the structure of which has not been established at the moment. Based on the fact that the main metabolite of tianeptine MC5 is isolated from the urine both at acidic pH values (pH = 3-4) and alkaline (pH = 9-10), it is possible to use as acidic as alkaline extracts for the chromatography-mass spectrometry analysis. Conclusions: The possibility of using gas chromatography and mass spectrometry methods for metabolites has been shown. The main metabolite of tianeptine can be isolated from the urine at acidic and also at alkaline pH values of the medium.

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A High-Performance Liquid Chromatography-Mass Spectrometry Method Development for the Quantitative Determination of Enalapril Maleate From Caco-2 Cell Monolayers

Cited by 3 publications

References 16 publications

Development of Methods of Analysis of Biological Active Substances for Standardization of Dense Extract of Creeping Thyme

Development of Methods of Analysis of Biological Active Substances for Standardization of Dense Extract of Creeping Thyme

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