Development and validation of determinative and confirmatory LC–MS/MS methodologies for total florfenicol and tulathromycin residues in bovine, equine and porcine kidney, liver and muscle tissues

Fedeniuk, Rick W.; McKenzie, Del; Mizuno, Massey; Neiser, Connie; O’Byrne, Collin; Shurmer, Bryn

doi:10.1016/j.jchromb.2014.12.035

Cited by 24 publications

(6 citation statements)

References 9 publications

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“…The method, however, has other steps that contribute to matrix elimination/reduction, including acetonitrile-based extraction that coextracts minimum lipids and other lipophilic compounds and precipitates proteins, which are then removed by centrifugation. Also, the solvent exchange of the evaporated 115 (39) 107 ( 23) 107 (19) 107 (15) 94.3 (32) 96.9 (23) 99.0 (12) 98.9 (15) 97.9 (9.2) 95.5 (6.2) sulfamethoxazole 101 (10) 113 (20) 87.8 (16) 86.3 (13) 97.9 (10) 110 (13) 109 ( 12…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

“…Veterinary drug residues can be determined using traditional methods that include immunoassays, , microbial inhibition assays (for antibiotics), , or liquid chromatography. − These methods often suffer from poor sensitivity and selectivity or involve multiple assays/analytical runs. Multiclass, multiresidue methods based on liquid chromatography–mass spectrometry (LC-MS) are becoming increasingly popular and required in regulatory monitoring programs globally owing to their extended analytical scope and laboratory efficiency; however, robust multiclass methods are still limited .…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Multiclass, Multiresidue Method for Veterinary Drug Analysis in Infant Formula and Related Ingredients Using UHPLC-MS/MS

Zhao

Zulkoski

Maštovská

2017

J. Agric. Food Chem.

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A multiclass, multiresidue method based on ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has been developed and validated for the analysis of around 150 veterinary drugs in infant formula and related dairy ingredients. The included analytes belong to the following veterinary drug classes: anthelmintics, antibiotics (aminoglycoside, amphenicols, β-lactams-penicillins and cephalosporins, lincosamides, macrolides, quinolones, sulfonamides, tetracyclines, and others), antimicrobial growth promoters, antiprotozoals, β-agonists, coccidiostats, dyes, pesticides, and tranquilizers. The sample preparation procedure involves dispersing the sample in 0.05 M EDTA solution in water, followed by extraction with 0.1% formic acid in acetonitrile, drying down an aliquot of the extract, and reconstituting it in a water-acetonitrile mixture. The analyte detection, identification, and quantitation are performed by UHPLC-MS/MS using positive electrospray ionization mode. The method was validated in infant formula powder, whole milk powder, and whey protein isolate, typically achieving limits of quantitation (meeting acceptable recovery and precision validation criteria) at 1-10 ng/g.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Multiclass, Multiresidue Method for Veterinary Drug Analysis in Infant Formula and Related Ingredients Using UHPLC-MS/MS

Zhao

Zulkoski

Maštovská

2017

J. Agric. Food Chem.

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“…Over the past years, several liquid chromatography mass spectrometry methods (LC‐MS/MS) have been developed to determine tulathromycin in different biological matrices, including plasma, 3,5–10 serum, 11,12 synovial fluid, 13 milk, 14 and other tissues 15–18 . However, to date, no LC‐MS/MS approaches have been validated in seminal plasma and urine.…”

Section: Introductionmentioning

confidence: 99%

“…[2][3][4] Over the past years, several liquid chromatography mass spectrometry methods (LC-MS/MS) have been developed to determine tulathromycin in different biological matrices, including plasma, 3,[5][6][7][8][9][10] serum, 11,12 synovial fluid, 13 milk, 14 and other tissues. [15][16][17][18] However, to date, no LC-MS/MS approaches have been validated in seminal plasma and urine. This could be useful to obtain information on its concentrations when used to treat genital tract infections, allowing to develop an appropriate and effective antibiotic therapy in animals.…”

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confidence: 99%

A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull

Barbarossa

Bardhi

Gazzotti

et al. 2022

Drug Testing and Analysis

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Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC-MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, seminal plasma, and urine. The approach is based on a quick protein precipitation with acetonitrile, filtration, and sample dilution before injection, allowing to rapidly process large batches of samples.Analytes separation was obtained using a BEH C18 (50 Â 2.1 mm, 1.7 μm) column, maintained at 40 C with a chromatographic run of 5 min. The method was fully validated over concentration ranges suitable for field levels of tulathromycin found in each matrix (0.01-1 μg/ml for plasma, 0.05-5 μg/ml for seminal plasma, and 0.1-10 μg/ml for urine), showing good linearity during each day of testing (R 2 always >0.99). Accuracy and precision were within ±15% at all QC concentrations in all the three matrices. Furthermore, the use of tulathromycine-d7 as internal standard mitigated the potential impacts of matrix effect. The validated technique was successfully applied to samples collected during a pharmacokinetic study in bulls, allowing to monitor tulathromycin concentrations over time in the three matrices. To our knowledge, this is the first validated approach for LC-MS/MS quantification of tulathromycin in seminal plasma and urine.

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“…Analytical methods, including high-performance liquid chromatography (HPLC) (Gáler et al, 2004;Huang et al, 2012) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Berrada, Borrull, Font, & Marcé, 2008;Fedeniuk et al, 2015;Hu et al, 2014;Scheuch et al, 2007) have already been used for the environmental analysis of TULA. HPLC methods have been routinely used for screening purposes but have disadvantages insofar as the method is complex, time consuming, and labour intensive (Liu, Suryoprabowo, Zheng, Song, & Kuang, 2017).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

Liu

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

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In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody-antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized the corresponding antigens produced in our laboratory. Under optimized conditions, the cut-off limits of the test strips for TULA in 0.01 M PBS pH 7.4 was found to be 2.5 ng/mL. Meanwhile, with simple preparation for detection in pure milk and honey samples, the cut-off limits for TULA were found to be 5 and 10 ng/mL, respectively. Each test can be evaluated in 5 min. In summary, this lateral-flow device provides a sensitive, effective, specific, and rapid method for detection of TULA residues in food matrix. ARTICLE HISTORY

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Development and validation of determinative and confirmatory LC–MS/MS methodologies for total florfenicol and tulathromycin residues in bovine, equine and porcine kidney, liver and muscle tissues

Cited by 24 publications

References 9 publications

Development and Validation of a Multiclass, Multiresidue Method for Veterinary Drug Analysis in Infant Formula and Related Ingredients Using UHPLC-MS/MS

Development and Validation of a Multiclass, Multiresidue Method for Veterinary Drug Analysis in Infant Formula and Related Ingredients Using UHPLC-MS/MS

A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull

Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

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