Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene &lt;I&gt;Pi25&lt;/I&gt;

Wang, Huimei; Chen, Jie; Shí, Yùzhēn; Pan, Gang; Shen, Hai-Chao; Wu, Jianli

doi:10.3724/sp.j.1006.2012.01960

Cited by 9 publications

(10 citation statements)

References 19 publications

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“…They both showed broader-spectrum resistances to pathogens and have been widely applied to rice breeding. Finally, considering that Hap_6, the most common haplotype of Pid3 , has been widely deployed in Chinese indica rice breeding especially in the major hybrid rice cultivars 56 , here we would suggest that it is the time to introduce some novel blast resistance genes (alleles) into the rice varieties with indica background 57 .…”

Section: Discussionmentioning

confidence: 96%

Allelic variation of the rice blast resistance gene Pid3 in cultivated rice worldwide

Huang

et al. 2017

Sci Rep

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In this study, the re-sequencing data from 3,000 rice genomes project (3 K RGP) was used to analyze the allelic variation at the rice blast resistance (R) Pid3 locus. A total of 40 haplotypes were identified based on 71 nucleotide polymorphic sites among 2621 Pid3 homozygous alleles in the 3k genomes. Pid3 alleles in most japonica rice accessions were pseudogenes due to premature stop mutations, while those in most indica rice accessions were identical to the functional haplotype Hap_6, which had a similar resistance spectrum as the previously reported Pid3 gene. By sequencing and CAPS marker analyzing the Pid3 alleles in widespread cultivars in China, we verified that Hap_6 had been widely deployed in indica rice breeding of China. Thus, we suggest that the priority for utilization of the Pid3 locus in rice breeding should be on introducing the functional Pid3 alleles into japonica rice cultivars and the functional alleles of non-Hap_6 haplotypes into indica rice cultivars for increasing genetic diversity.

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Section: Discussionmentioning

confidence: 96%

Allelic variation of the rice blast resistance gene Pid3 in cultivated rice worldwide

Huang

et al. 2017

Sci Rep

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“…Overexpression analysis and complementation tests showed that Pi25 is the only gene that confers resistance to M. oryzae isolate js001-20 (Chen et al 2011). In addition, a low frequency of 1.2% (2 of 167) of the dominant allele was detected in germplasm collections, indicating this gene has not been fully utilized in rice breeding programs in China (Wang et al 2012). …”

Section: Discussionmentioning

confidence: 97%

Identification of rice blast resistance genes in the elite hybrid rice restorer line Yahui2115

Shi

et al. 2015

Genome

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Rice blast, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most serious rice diseases worldwide. We previously developed an elite hybrid rice restorer line with high resistance to rice blast, Yahui2115 (YH2115). To identify the blast resistance genes in YH2115, we first performed expression profiling on previously reported blast resistance genes and disease assay on monogenic lines, and we found that Pi2, Pi9, and Pikm were the most likely resistance candidates in YH2115. Furthermore, RNA interference and linkage analysis demonstrated that silencing of Pi2 reduced the blast resistance of YH2115 and a Pi2 linkage marker was closely associated with blast resistance in an F2 population generated from YH2115. These data suggest that the broad-spectrum blast resistance gene Pi2 contributes greatly to the blast resistance of YH2115. Thus, YH2115 could be used as a new germplasm to facilitate rice blast resistance breeding in hybrid rice breeding programs.

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“…Genomic DNA samples of two-week-old rice leaves and M. oryzae mycelia were extracted using the CTAB method [ 21 ]. The functional or diagnostic markers listed in Tables S3 and S4 were used to detect the genotypes of 18 cloned blast R genes ( Pib , Pikh , Pita , Pid3 , Pid2 , Pia , Pik , Pi-zt , Pb1 , Pikm , Pi3/5/I , Pi1 , Pit , Pi25 , Pigm , Piz , Pi9 , Pi2 ) [ 17 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ] in rice and 10 isolated avirulence genes ( Avr1-Co39 , Avri-Pita , ACE1 , Avr-Pib , PWL1 , Avr-Pia , Avr-Pi9 , Avr-Pizt , Avr-Pik , and Avr-Pii ) in M. oryzae strains [ 37 , 38 , 39 , 40 , 41 ]. PCR products were separated by electrophoresis on 4% agarose gels or 8% non-deformable polyacrylamide gels.…”

Section: Methodsmentioning

confidence: 99%

Identification of Elite R-Gene Combinations against Blast Disease in Geng Rice Varieties

Gao

Wang

et al. 2023

IJMS

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Rice blast, caused by the Magnaporthe oryzae fungus, is one of the most devastating rice diseases worldwide. Developing resistant varieties by pyramiding different blast resistance (R) genes is an effective approach to control the disease. However, due to complex interactions among R genes and crop genetic backgrounds, different R-gene combinations may have varying effects on resistance. Here, we report the identification of two core R-gene combinations that will benefit the improvement of Geng (Japonica) rice blast resistance. We first evaluated 68 Geng rice cultivars at seedling stage by challenging with 58 M. oryzae isolates. To evaluate panicle blast resistance, we inoculated 190 Geng rice cultivars at boosting stage with five groups of mixed conidial suspensions (MCSs), with each containing 5–6 isolates. More than 60% cultivars displayed moderate or lower levels of susceptibility to panicle blast against the five MCSs. Most cultivars contained two to six R genes detected by the functional markers corresponding to 18 known R genes. Through multinomial logistics regression analysis, we found that Pi-zt, Pita, Pi3/5/I, and Pikh loci contributed significantly to seedling blast resistance, and Pita, Pi3/5/i, Pia, and Pit contributed significantly to panicle blast resistance. For gene combinations, Pita+Pi3/5/i and Pita+Pia yielded more stable pyramiding effects on panicle blast resistance against all five MCSs and were designated as core R-gene combinations. Up to 51.6% Geng cultivars in the Jiangsu area contained Pita, but less than 30% harbored either Pia or Pi3/5/i, leading to less cultivars containing Pita+Pia (15.8%) or Pita+Pi3/5/i (5.8%). Only a few varieties simultaneously contained Pia and Pi3/5/i, implying the opportunity to use hybrid breeding procedures to efficiently generate varieties with either Pita+Pia or Pita+Pi3/5/i. This study provides valuable information for breeders to develop Geng rice cultivars with high resistance to blast, especially panicle blast.

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Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene <I>Pi25</I>

Cited by 9 publications

References 19 publications

Allelic variation of the rice blast resistance gene Pid3 in cultivated rice worldwide

Allelic variation of the rice blast resistance gene Pid3 in cultivated rice worldwide

Identification of rice blast resistance genes in the elite hybrid rice restorer line Yahui2115

Identification of Elite R-Gene Combinations against Blast Disease in Geng Rice Varieties

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