Development and validation of an RP-HPLC method for estimation of famciclovir in bulk and pharmaceutical formulations

Abstract

A reverse phase HPLC method is described for the determination of famciclovir in bulk and tablets. Chromatography was carried on a C 18 column using a mixture of acetonitrile, and glacial acetic acid (70:30 v/v) as the mobile phase at a flow rate of 1 ml min-1 with detection at 310 nm. The retention time of the drug was 3.56 min. The detector response was linear in the concentration of 1-40 μg/ml. The limit of detection and limit of quantification was 0.335 and 1.014 μg ml-1 respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of famciclovir in bulk and tablets.