Development and validation of an UPLC-UV method for determination of a novel triazine coccidiostat ethanamizuril and its metabolite M3 in chicken tissues

Zhao, Xiaochun; Xu, Yue; Zhang, Lifang; Wang, Chunmei; Guo, Chunna; Fei, Chenzhong; Zhang, Keyu; Wang, Xiaoyang; Liu, Yingchun; Wang, Mi; Zheng, Wenli; Xue, Feiqun

doi:10.1016/j.jchromb.2017.04.049

Cited by 18 publications

(12 citation statements)

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“…The structure of N‐(4‐(4‐(3,5‐dioxo‐4,5‐dihydro‐1,2,4‐triazin‐2‐[3H]‐yl)‐2‐methylphenoxy) phenyl) acetamide, also known as ethanamizuril (CAS:1560840–75‐6, Figure 1), is similar to that of diclazuril and toltrazuril and has been developed independently in recent years by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences (Cheng et al, 2018; Zhang et al, 2014; Zhao et al, 2017). Ethanamizuril exhibits remarkable anticoccidial activity when supplemented at 7–10 ppm in the tap water or 7–10 ppm in the diet on 2–3 consecutive days and only a few fecal oocysts and slight caecal lesions are observed after treatment of infected chickens.…”

Section: Introductionmentioning

confidence: 99%

Two‐generation reproduction and limited teratology studies of ethanamizuril fed to rats

Zhao

Wang

et al. 2020

Birth Defects Research

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Ethanamizuril, a new anticoccidial agent that belongs to triazine derivatives, has a broad and good anticoccidial activity. To evaluate the reproductive toxicity and teratogenic potential of ethanamizuril, different concentrations of ethanamizuril were administered to Sprague-Dawley rats by feeding diets containing 0, 2, 8, and 30 ppm, respectively. Each group consisting of 30 males and 30 females (F0) was treated with different concentrations of ethanamizuril through a 13-week period before mating and during mating, gestation, parturition and lactation. In the 30 ppm dose group, pup body weight on days 7 and 21 in F1 offspring and day 21 in the F2a offspring were significantly decreased.A limited teratogenicity study was performed in combination with the F1 generation of a two-generation reproduction study. F1 offspring of the reproduction study were mated after weaning of the F2a offspring. Pregnant female rats were subjected to cesarean section on gestational day 20 for teratogenic examination. No obvious body weights, fetal body lengths, tail lengths, litter weights, number of viable fetuses, external, skeletal, or visceral malformations in fetuses were noted in any groups in the teratogenic test, but ethanamizuril could be passed on to offspring through lactation. K E Y W O R D S ethanamizuril, feeding in diet, reproductive toxicity, Sprague-Dawley rats, teratogenicity

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Section: Introductionmentioning

confidence: 99%

Two‐generation reproduction and limited teratology studies of ethanamizuril fed to rats

Zhao

Wang

et al. 2020

Birth Defects Research

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“…Ethanamizuril (Fig. 1), a novel triazine coccidiostat, namely (N-(4-(4-(3,5-dioxo-4,5-dihydro-1,2,4-triazin-2-(3H)-yl)-2-methylphenoxy) phenyl)acetamide, has been independently established by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences in recent years and exert wide application prospective in future [5,7]. In China and Japan Patents, the chemical synthesis of ethanamizuril and the chemical structure of this compound have been published [8].…”

Section: Introductionmentioning

confidence: 99%

Beagle dog 90-day oral toxicity study of a novel coccidiostat – ethanamizuril

et al. 2020

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Background Triazine coccidiostats are widely used in chickens and turkeys for coccidiosis control. Ethanamizuril is a novel triazine compound that exhibits anticoccidial activity in poultry. This study was designed to evaluate the subchronic toxicity of ethanamizuril in beagle dogs at doses of 12, 60 or 300 mg/kg/day in diet for 90 days. Results Ethanamizuril was well tolerated at low and middle dosages in beagle dogs, and no drug-related toxical effects were observaed in terms of survival, clinical observations, organs weight and damage in these dose groups. However, in high dose administration group, food consumption and histologic changes in kidneys were noticed in both sexes of beagle dog, although the renal lesions were finally resolved at the end of 4 weeks exposure of ethanamizuril. Conclusions No-observed-adverse-effect level (NOAEL) was considered for ethanamizuril at dose of 60 mg/kg/day in Beagle dog. This result added toxicity effects of ethanamizuril to the safety database, which might guide safely using of ethanamizuril as a novel coccidiostat.

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“…Ethanamizuril (Figure 1), a novel triazine coccidiostat, namely (N-(4-(4-(3,5-dioxo-4,5-dihydro-1,2,4-triazin-2-(3H)-yl)-2-methylphenoxy) phenyl)acetamide, has been independently established by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences in recent years and exert wide application prospective in future [5,7]. In China and Japan Patents, the chemical synthesis of ethanamizuril and the chemical structure have been published [8].…”

Section: Introductionmentioning

confidence: 99%

Beagle dog 90-day oral toxicity study of a novel coccidiostat – ethanamizuril

Zhang

Wang

Wei

et al. 2020

Preprint

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Background: Triazine coccidiostats are widely used in chickens and turkeys for coccidiosis control. Ethanamizuril is a novel triazine compound that exhibits anticoccidial activity in poultry. This study evaluated the subchronic toxicity of ethanamuzuril in beagle dogs administered ethanamizuril by diet at doses of 12, 60 or 300 mg/kg/day for 90 days.Results: Ethanamizuril was well tolerated at low and middle dosages and in these dose groups there were no ethanamizuril related effects on survival, clinical observations, clinical pathology parameters, organs weight, macroscopic or microscopic evaluations. The ethanamizuril related changes were limited to effects on food consumption and histologic changes of kidneys in the 300 mg/kg/day group in both sexes. However, renal lesions resolved in dogs given ethanamizuril at 300 mg/kg/day when evaluated four weeks after the end of exposure.Conclusions: Therefore, the no-observed-adverse-effect level (NOAEL) was considered to be 60 mg/kg/day, the middle dosage level tested. These results add to the safety database for ethanamizuril with potential for use as a novel coccidiostat.

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Development and validation of an UPLC-UV method for determination of a novel triazine coccidiostat ethanamizuril and its metabolite M3 in chicken tissues

Cited by 18 publications

References 8 publications

Two‐generation reproduction and limited teratology studies of ethanamizuril fed to rats

Two‐generation reproduction and limited teratology studies of ethanamizuril fed to rats

Beagle dog 90-day oral toxicity study of a novel coccidiostat – ethanamizuril

Beagle dog 90-day oral toxicity study of a novel coccidiostat – ethanamizuril

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