Beagle dog 90-day oral toxicity study of a novel coccidiostat – ethanamizuril

Abstract

Background Triazine coccidiostats are widely used in chickens and turkeys for coccidiosis control. Ethanamizuril is a novel triazine compound that exhibits anticoccidial activity in poultry. This study was designed to evaluate the subchronic toxicity of ethanamizuril in beagle dogs at doses of 12, 60 or 300 mg/kg/day in diet for 90 days. Results Ethanamizuril was well tolerated at low and middle dosages in beagle dogs, and no drug-related toxical effects were observaed in terms of survival, clinical observations, organs weight and damage in these dose groups. However, in high dose administration group, food consumption and histologic changes in kidneys were noticed in both sexes of beagle dog, although the renal lesions were finally resolved at the end of 4 weeks exposure of ethanamizuril. Conclusions No-observed-adverse-effect level (NOAEL) was considered for ethanamizuril at dose of 60 mg/kg/day in Beagle dog. This result added toxicity effects of ethanamizuril to the safety database, which might guide safely using of ethanamizuril as a novel coccidiostat.

Effects of salinomycin and ethanamizuril on the three microbial communities in vivo and in vitro

“…Triazines have been reported to inhibit the nuclear division of protozoan parasites, thereby interfering with the development and growth of schizonts and gametocytes (Stock et al, 2018). Ethanamizuril is a novel triazine compound that has shown excellent effectiveness against coccidiosis at a dosage of 10 mg/L in drinking water (Wang et al, 2020;Zhang et al, 2020b). It has been approved for usage in broiler chickens since 2020 in China.…”