Development and validation of an HPLC–UV method for the quantification of carbamazepine in rabbit plasma

Mowafy, Hammam A.; Alanazi, Fars K.; Maghraby, Gamal M. El

doi:10.1016/j.jsps.2011.04.003

Cited by 47 publications

(22 citation statements)

References 34 publications

(33 reference statements)

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“…A literature review revealed that different methods have been reported for the determination of CBZ. These include spectrophotometry , photoinduced fluorimetry , voltammetry , high‐performance liquid chromatography , spectrofluorimetry , micellar electrokinetic capillary chromatography , solid‐phase extraction , chemiluminescence , liquid chromatography , ion chromatography and gas chromatographic methods .…”

Section: Introductionmentioning

confidence: 99%

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

2015

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A sensitive and simple spectrofluorimetric method has been developed and validated for the determination of the anti-epileptic drug carbamazepine (CBZ) in its dosage forms. The method was based on a nucleophilic substitution reaction of CBZ with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer (pH 9) to form a highly fluorescent derivative that was measured at 530 nm after excitation at 460 nm. Factors affecting the formation of the reaction product were studied and optimized, and the reaction mechanism was postulated. The fluorescence-concentration plot is rectilinear over the range of 0.6-8 µg/mL with limit of detection of 0.06 µg/mL and limit of quantitation of 0.19 µg/mL. The method was applied to the analysis of commercial tablets and the results were in good agreement with those obtained using the reference method. Validation of the analytical procedures was evaluated according to ICH guidelines.

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Section: Introductionmentioning

confidence: 99%

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

2015

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“…A great merit of this method is the opportunity to determine the structure and the level of impurity in drugs and formulations. This opportunity is not limited to synthetic drugs and can be applied to herbal drugs, too (32,33).…”

Section: Discussionmentioning

confidence: 99%

Developing a High-Performance Liquid Chromatography Fast and Accurate Method for Quantification of Silibinin In-Vitro

Bakhshi

Molavi

Rashidi

et al. 2019

Preprint

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Objective : Silibinin is an antioxidant agent and is shown to have anticancer effects in different cancers including lung, breast, colorectal, liver, prostate, and kidney. There are challenges in the clinical use of Silibinin. The main limitation is low solubility, poor oral absorption, and extensive hepatic metabolism. We developed a High-Performance Liquid Chromatography sensitive method for quantification of Silibinin in in-vitro samples to quantify in different formulations. We applied HPLC set, C18 column as stationary phase and the mixture of methanol (90%) and water (10%) as mobile phase. Utilized solutions were daily prepared through serial dilution of stock solution to make specific concentrations ranged between 10 μg/ml to 100 μg/ml. The developed method was validated by credible guidelines.Results : The calibration curve was drawn according to the height peaks of different concentrations and the established method demonstrated a linear ranged from 10 to 100 µg/ml, with a correlation coefficient of 0.996 for in-vitro samples. The retention time was 2.97 min, and the accuracy and precision (inter-day and intra-day) of the developed method was in the range of 88-105.9%. These results demonstrate that the developed method can be a fast and accurate method for quantification of Silibinin in in-vitro samples.

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“…An isocratic simple rapid assay has been developed and validated for the determination of CBZ in both solution form and rabbit plasma [85] using propylparaben as an internal standard. The assay was performed using a µ-Bondapak C 18 (150 mm×4.6 mm) with a mobile phase consisting of methanol and water (50:50, v/v), at a flow rate of 1 mL/min and UV detection at 285 nm.…”

Section: Hplcmentioning

confidence: 99%

Quantitative bioanalytical and analytical method development of dibenzazepine derivative, carbamazepine: A review

Datar¹

2015

Journal of Pharmaceutical Analysis

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Bioanalytical methods are widely used for quantitative estimation of drugs and their metabolites in physiological matrices. These methods could be applied to studies in areas of human clinical pharmacology and toxicology. The major bioanalytical services are method development, method validation and sample analysis (method application). Various methods such as GC, LC–MS/MS, HPLC, HPTLC, micellar electrokinetic chromatography, and UFLC have been used in laboratories for the qualitative and quantitative analysis of carbamazepine in biological samples throughout all phases of clinical research and quality control. The article incorporates various reported methods developed to help analysts in choosing crucial parameters for new method development of carbamazepine and its derivatives and also enumerates metabolites, and impurities reported so far.

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Development and validation of an HPLC–UV method for the quantification of carbamazepine in rabbit plasma

Cited by 47 publications

References 34 publications

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

Developing a High-Performance Liquid Chromatography Fast and Accurate Method for Quantification of Silibinin In-Vitro

Quantitative bioanalytical and analytical method development of dibenzazepine derivative, carbamazepine: A review

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