Development and validation of an HPLC-MS/MS method with QuEChERS extraction using isotopic dilution to simultaneously analyze chlordecone and chlordecol in animal livers

Development and Application of Molecularly Imprinted Polymers for the Selective Extraction of Chlordecone from Bovine Serum

“…Today, GC is still used but coupled with mass spectrometry for its higher specificity, especially at low concentrations [10]. However, more recent studies use liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) preceded, or not, by an isotopic dilution for quantifying CLD in food, tissues, or biological fluids leading to an LOQ of only a few µg kg −1 [11][12][13].…”

“…Then, an additional cleanup of the organic layer is performed with NaOH treatment and acidic lysis with sulfuric acid [10] or with a solid-phase extraction (SPE) step on Florisil ® cartridges [15]. More recently, a method dedicated to the analyses of CLD in animal liver [11,12], feces, and urine of ewes [11,12], based on QuEChERS method, was validated. Concerning the extraction procedure for blood samples (serum [13] or plasma [16]), it is based on a slightly simpler protocol originally dedicated to the extraction of polychlorobiphenyls (PCB) [15].…”

Simultaneous determination of 114 pesticides in complex Chinese herbal medicine Fritillaria using ordered mesoporous carbon CMK-3 as a reversed-dispersive solid phase extraction sorbent

“…CMK-3 was selected as a sorbent to remove alkaloids, and PSA was selected to remove polar substances such as fatty acids in the sample matrix. 39,40 The amounts of CMK-3 and PSA were optimized to obtain the most efficient purication effect and pesticide recoveries. In the following optimization experiments, 6 mL of ACN was used to extract the analytes in the spiked FT samples at a concentration of 120 mg kg À1 , and 1 mL of the obtained extraction solutions was drawn for purication analysis.…”

Correlation between endemic chlordecone concentrations in three bovine tissues determined by isotopic dilution liquid chromatography–tandem mass spectrometry

“…Although gas chromatography is still used, most of the current methods, including the official method for controls in France, are based on isotopic dilution followed by reversed-phase liquid chromatography analysis and tandem mass spectrometry detection with negative electrospray ionization (Clostre et al, 2015;Lastel et al, 2018;Saint-Hilaire et al, 2018). Isotopic dilution is recommended to ensure accurate identification and quantification.…”