Development and validation of a liquid chromatography–fluorescence–mass spectrometry method to measure glyphosate and aminomethylphosphonic acid in rat plasma

Bernal, José; Martín, M.T.; Nozal, Marı́a J.; Anadón, Arturo; Martínez-Larrañaga, María-Rosa; Martínez, Marta

doi:10.1016/j.jchromb.2010.10.013

Cited by 43 publications

(23 citation statements)

References 45 publications

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“…In addition, the applicability of derivatizing agents should be evaluated for every matrix; the derivatization technique developed for one matrix may not work with a different matrix. For example, a method using LC‐FLD‐ESI‐MS to determine glyphosate and AMPA in one matrix (rat plasma) had to be adapted and the FMOC derivatization step modified for use with a new matrix (maize) . Also, if FMOC, the most commonly used derivatizing agent, is not removed, excess FMOC will interfere with AMPA quantitation when an –NH 2 anion exchange column is used for separation .…”

Section: Discussionmentioning

confidence: 99%

“…UV, FLD), and to lower levels of detection in water, pre‐ and post‐column derivatization methods have been used. During the past 15 years, the most commonly used derivatizing reagent has been 9‐fluorenyl methoxycarbonyl chloride (FMOC‐Cl) . FMOC‐Cl was used in about two‐thirds of the published methods that relied upon derivatizing agents (Table ).…”

Section: Analysis Methodsmentioning

confidence: 99%

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Analysis of glyphosate and aminomethylphosphonic acid in water, plant materials and soil

2015

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There is a need for simple, fast, efficient and sensitive methods of analysis for glyphosate and its degradate aminomethylphosphonic acid (AMPA) in diverse matrices such as water, plant materials and soil to facilitate environmental research needed to address the continuing concerns related to increasing glyphosate use. A variety of water-based solutions have been used to extract the chemicals from different matrices. Many methods require extensive sample preparation, including derivatization and clean-up, prior to analysis by a variety of detection techniques. This review summarizes methods used during the past 15 years for analysis of glyphosate and AMPA in water, plant materials and soil. The simplest methods use aqueous extraction of glyphosate and AMPA from plant materials and soil, no derivatization, solid-phase extraction (SPE) columns for clean-up, guard columns for separation and confirmation of the analytes by mass spectrometry and quantitation using isotope-labeled internal standards. They have levels of detection (LODs) below the regulatory limits in North America. These methods are discussed in more detail in the review.

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Methodsmentioning

confidence: 99%

Analysis of glyphosate and aminomethylphosphonic acid in water, plant materials and soil

2015

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“…Treatment groups had significant primary DNA damage in the liver cells and leukocytes. Acetylcholinesterase (AChE) activity was found to be inhibited with all the treatments suggesting that even low doses of glyphosate exposure can cause serious adverse effects [ 50 ]. Glyphosate undergoes one-compartment model with an elimination rate constant of K el = 0.021 h −1 when 100 mg/kg bw) of glyphosate concentrations in plasma in Sprague-Dawley rats [ 51 ].…”

Section: Glyphosate Toxicitymentioning

confidence: 99%

Herbicide Glyphosate: Toxicity and Microbial Degradation

Singh

Kumar

Gill³

et al. 2020

IJERPH

124

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Glyphosate is a non-specific organophosphate pesticide, which finds widespread application in shielding crops against the weeds. Its high solubility in hydrophilic solvents, especially water and high mobility allows the rapid leaching of the glyphosate into the soil leading to contamination of groundwater and accumulation into the plant tissues, therefore intricating the elimination of the herbicides. Despite the widespread application, only a few percentages of the total applied glyphosate serve the actual purpose, dispensing the rest in the environment, thus resulting in reduced crop yields, low quality agricultural products, deteriorating soil fertility, contributing to water pollution, and consequently threatening human and animal life. This review gives an insight into the toxicological effects of the herbicide glyphosate and current approaches to track and identify trace amounts of this agrochemical along with its biodegradability and possible remediating strategies. Efforts have also been made to summarize the biodegradation mechanisms and catabolic enzymes involved in glyphosate metabolism.

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“…According to this selection, out of twenty protocols, independent on the matrix tested, in twelve [48,[50][51][52]54,55,[57][58][59]61,64,66], the common enrichment technologies (SPE, anion or cation exchange in aqueous solutions) are followed by using FMOC; fluorescent derivatives of high responses are formed, in Indications, as in Tables 1A, 1B, aqueous solutions, mostly at pH 9, under mild conditions (from room temperature up to 40°C, from 20 min up to 24 h). Next to FMOC the use of the OPA-MCE reagent, providing also fluorescent derivatives, revealed applicability in practical use [49,56,63], primarily for detection, only.…”

Section: Identification and Quantification As Various Derivativesmentioning

confidence: 99%

The role of derivatization techniques in the analysis of glyphosate and aminomethyl-phosphonic acid by chromatography

Arkan

Molnár-Perl

2015

Microchemical Journal

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Development and validation of a liquid chromatography–fluorescence–mass spectrometry method to measure glyphosate and aminomethylphosphonic acid in rat plasma

Cited by 43 publications

References 45 publications

Analysis of glyphosate and aminomethylphosphonic acid in water, plant materials and soil

Analysis of glyphosate and aminomethylphosphonic acid in water, plant materials and soil

Herbicide Glyphosate: Toxicity and Microbial Degradation

The role of derivatization techniques in the analysis of glyphosate and aminomethyl-phosphonic acid by chromatography

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