Development and validation of a sensitive solid phase extraction/hydrophilic interaction liquid chromatography/mass spectrometry method for the accurate determination of glucosamine in dog plasma

Hubert, Cédric; Houari, Sabah; Lecomte, Frédéric; Houbart, Virginie; Bleye, Charlotte De; Fillet, Marianne; Piel, Géraldine; Rozet, Eric; Hubert, Ph.

doi:10.1016/j.chroma.2010.01.024

Cited by 23 publications

(17 citation statements)

References 34 publications

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“…This was consistent with previously reference [37]. In this sense, quantifications of glucosamine in urines by LIF detection were more reliable compared with TOF-MS detector.…”

Section: Determination Of Glucosamine In Urinessupporting

confidence: 91%

“…The chromatographic determination of glucosamine has been frequently coupled with pre-column derivatization [33][34][35]. Its quantification was reported by tandem triple quadrupole MS, and achieved sensitivities, however, were worse than its counterparts by LIF [36,37]. In this study, proposed HPLC-LIF/UV/TOF-MS was evaluated in the determination of glucosamine in urines.…”

Section: Introductionmentioning

confidence: 97%

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Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromatography and determination of glucosamine in urine

Xiong

Wang

et al. 2015

Talanta

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“…This was consistent with previously reference [37]. In this sense, quantifications of glucosamine in urines by LIF detection were more reliable compared with TOF-MS detector.…”

Section: Determination Of Glucosamine In Urinessupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromatography and determination of glucosamine in urine

Xiong

Wang

et al. 2015

Talanta

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“…An alternative separation mode of chromatography is hydrophilic interaction liquid chromatography (HILIC) on a propyl-cyano column with MS detection (Zhong, Zhong, & Chen, 2007;Hubert et al, 2010;César et al, 2012;Yang, Zheng, Simpemba, Ma, & Ding, 2015). HILIC for glucosamine determination has also been reported on a propyl-amino column with refractive index detection (without derivatization; El-Saharty & Bary, 2002;Xinmin, Ruili, Zhihua, Yuanhong, & Tingfu, 2008), or with UV-vis detection after derivatization (Liang, Leslie, Adebowale, Ashraf, & Eddington, 1999).…”

Section: Various Methods For Determination Of Glucosaminementioning

confidence: 99%

Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector

Pazourek

2018

Biomedical Chromatography

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Saccharides and their derivatives are typical polar analytes without a suitable UV-chromophore that are nowadays analyzed by HPLC (high-performance liquid chromatography) under HILIC (hydrophilic interaction liquid chromatography) mode. Usually an evaporative light scattering detector (ELSD) is utilized which, however, gives a nonlinear response. A procedure to overcome the problem of mutarotating (time-varying) analytes recorded with such a nonlinear response detector is described. The procedure was applied for determination of glucosamine in two commercially available pharmaceutical formulations containing the common inorganic ions that the detector gives a response to. Under optimized conditions, both the anomers of glucosamine were separated and could be determined separately. Owing to the short retention time of the analyte (a run time <4 min) and relatively slow kinetics of the anomeric conversion (equilibration time 2.5 h), mutarotation could be monitored and corresponding rate constants calculated.

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“…The lack of sensitive analytical methods capable of detecting the compound in biological fluids has been the primary limitation of pharmacokinetic studies. In addition, other major difficulties associated with the quantitative determination of GlcN in biological samples are low GlcN levels and the presence of many structurally related sugar molecules that can interfere with its analysis [33,34]. …”

Section: Discussionmentioning

confidence: 99%

Physiological effects of oral glucosamine on joint health: current status and consensus on future research priorities

et al. 2013

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The aim of this paper was to provide an overview of the current knowledge and understanding of the potential beneficial physiological effects of glucosamine (GlcN) on joint health. The objective was to reach a consensus on four critical questions and to provide recommendations for future research priorities. To this end, nine scientists from Europe and the United States were selected according to their expertise in this particular field and were invited to participate in the Hohenheim conference held in August 2011. Each expert was asked to address a question that had previously been posed by the chairman of the conference. Based on a systematic review of the literature and the collection of recent data, the experts documented the effects of GlcN on cartilage ageing, metabolic/kinetic and maintenance of joint health as well as reduction of risk of OA development. After extensive debate and discussion the expert panel addressed each question and a general consensus statement was developed, agreeing on the current state-of-the-art and future areas for basic and clinical studies. This paper summarizes the available evidence for beneficial effects of GlcN on joint health and proposes new insight into the design of future clinical trials aimed at identifying beneficial physiological effect of GlcN on joint tissues.

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Development and validation of a sensitive solid phase extraction/hydrophilic interaction liquid chromatography/mass spectrometry method for the accurate determination of glucosamine in dog plasma

Cited by 23 publications

References 34 publications

Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromatography and determination of glucosamine in urine

Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromatography and determination of glucosamine in urine

Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector

Physiological effects of oral glucosamine on joint health: current status and consensus on future research priorities

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