Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of goserelin in rabbit plasma

Kim, Min Kyung; Lee, Tae Ho; Suh, Joon Hyuk; Eom, Han Young; Min, Jung Won; Yeom, Hyesun; Kim, Unyong; Jung, Hyung Joon; Hoi, Kyung; Choi, Yong Seok; Youm, Jeong-Rok; Han, Sang Beom

doi:10.1016/j.jchromb.2010.06.031

Cited by 17 publications

(5 citation statements)

References 33 publications

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“…The peak-area ratios of the samples were then interpolated from the standard curve to obtain the concentrations of ardipusilloside I. The sensitivity of the method was expressed as the lower limit of quantification, which was determined as the lowest plasma concentration with acceptable accuracy (80-120 %) and precision (< 20% relative standard deviation) with a signal/noise ratio ≥ 10 [7]. The matrix effect was evaluated by comparing the peak area of blank plasma spiked with ardipusilloside I or ginsenoside Re to the peak area of methanol spiked with the same concentration of ardipusilloside I or ginsenoside Re.…”

Section: Linearity Sensitivity and Matrix Effect In Ardipusillosidementioning

confidence: 99%

Metabolism and Pharmacokinetic Study of Ardipusilloside I in Rats

Wang

Cui²,

Wang³

et al. 2012

Planta Med

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Ardipusilloside I, extracted from ARDISIA PUSILLA A.DC, effectively inhibits the progression of several cancers in animal models and is a potential anti-cancer drug candidate. However, the metabolism and pharmacokinetic characteristics of ardipusilloside I remain unknown. In this study, we developed a highly sensitive liquid chromatography-tandem MS method to determine the ardipusilloside I concentration in rat plasma using ginsenoside Re (whose structure is similar to ardipusilloside I) as the internal standard. After oral administration of ardipusilloside I, its four possible metabolites (M1, M2, M3, and M4, whose structures were determined by MS) were detected in the content from rat small intestine. In rat plasma, however, only M3 and M4 were detected after oral administration of ardipusilloside I. None of the metabolites were detected in plasma samples after intravenous administration of ardipusilloside I to rats. These results indicated that the metabolites, but not the drug itself, were absorbed into plasma after oral administration of ardipusilloside I to rats and that M3 and M4 may be responsible for the antitumor activity of orally administered ardipusilloside I in rat models of cancer.

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Section: Linearity Sensitivity and Matrix Effect In Ardipusillosidementioning

confidence: 99%

Metabolism and Pharmacokinetic Study of Ardipusilloside I in Rats

Wang

Cui²,

Wang³

et al. 2012

Planta Med

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“…В приведенных работах [11][12][13] твердофазная экстракция (ТФЭ) является основным методом пробоподготовки для достижения более низких значений нПКО. Альтернативным вариантом был выбран метод осаждения белков, где в качестве осаждающих реактивов применялись метанол, ацетонитрил и 10% раствор трихлоруксусной кислоты (ТХУ).…”

Section: доклинические и клинические исследования Preclinical and CLIunclassified

Development and Validation of HPLC-MS/MS Method for Busereline Quantitation in Animal Blood Plasma

Fisher¹,

Мельников²,

Шохин³

2019

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Busereline, being a synthetic gonadotropin-releasing hormone analog, is widely used for hormone-dependent cancer treatment (e.g. prostate cancer and breast cancer). Based on the accumulated scientific data for busereline quantitation in biosamples, the main analytical method that is used for this purpose is high-performance liquid chromatography (HPLC) with fluorescence detection, combined with protein precipitation (TCA 10%) for sample preparation. However, due to several limitations of this method resulting in low sensitivity (at the µg/mL level of concentrations), the HPLC-MS/MS analytical method was chosen for peptide determination in biosamples. The HPLC-MS/MS method is considered to have higher accuracy and specificity. The main sample preparation method for gonadotropin-releasing hormone analogs is solid-phase extraction. In our work, we’ve chosen protein precipitation as an alternative – easier and less laborious biosamples preparation process.Aim. The main objective of this study was the development and validation of HPLC-MS/MS method for busereline quantitation in animal (mini pigs) plasma samples and its further application to pharmacokinetic studies.Materials and methods. Busereline quantitative determination in plasma samples was performed using HPLC-MS/MS method. A protein precipitation procedure (methanol, 1:2, v/v) was used for busereline extraction from pig plasma.Results and discussion. The developed analytical method was validated for selectivity, linearity, matrix effect, accuracy (intra-day, inter-day), precision (intra-day, inter-day), LLOQ, carryover and stability.Conclusion. A new HPLC-MS/MS method for busereline quantitation in blood plasma was developed and successfully validated. The developed method showed linearity over the quantitation range from 1 to 20 ng/mL. The developed method can be successfully applied to busereline pharmacokinetic studies.

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“…Several MS methods have been described for pharmacokinetic studies to detect and quantify GnRH‐agonists or ‐stimulators, such as triptorelin, goserelin, and kisspeptin‐10 in biofluids (Kim et al, ; Han et al, ; Liu et al, ). In addition, several MS assays to detect abuse of GnRH in human urine for sports‐doping control analysis have been described.…”

Section: Top‐down Ms Detection Of Growth‐promoting Peptides and Proteinsmentioning

confidence: 99%

Current trends in mass spectrometry of peptides and proteins: Application to veterinary and sports‐doping control

Broek

Blokland

Nessen

et al. 2013

Mass Spectrometry Reviews

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Detection of misuse of peptides and proteins as growth promoters is a major issue for sport and food regulatory agencies. The limitations of current analytical detection strategies for this class of compounds, in combination with their efficacy in growth-promoting effects, make peptide and protein drugs highly susceptible to abuse by either athletes or farmers who seek for products to illicitly enhance muscle growth. Mass spectrometry (MS) for qualitative analysis of peptides and proteins is well-established, particularly due to tremendous efforts in the proteomics community. Similarly, due to advancements in targeted proteomic strategies and the rapid growth of protein-based biopharmaceuticals, MS for quantitative analysis of peptides and proteins is becoming more widely accepted. These continuous advances in MS instrumentation and MS-based methodologies offer enormous opportunities for detection and confirmation of peptides and proteins. Therefore, MS seems to be the method of choice to improve the qualitative and quantitative analysis of peptide and proteins with growth-promoting properties. This review aims to address the opportunities of MS for peptide and protein analysis in veterinary control and sports-doping control with a particular focus on detection of illicit growth promotion. An overview of potential peptide and protein targets, including their amino acid sequence characteristics and current MS-based detection strategies is, therefore, provided. Furthermore, improvements of current and new detection strategies with state-of-the-art MS instrumentation are discussed for qualitative and quantitative approaches.

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Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of goserelin in rabbit plasma

Cited by 17 publications

References 33 publications

Metabolism and Pharmacokinetic Study of Ardipusilloside I in Rats

Metabolism and Pharmacokinetic Study of Ardipusilloside I in Rats

Development and Validation of HPLC-MS/MS Method for Busereline Quantitation in Animal Blood Plasma

Current trends in mass spectrometry of peptides and proteins: Application to veterinary and sports‐doping control

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