Development and validation of a UHPLC-HRMS-QTOF method for the detection of 132 New Psychoactive Substances and synthetic opioids, including fentanyl, in Dried Blood Spots

Massano, Marta; Incardona, Carola; Gerace, Enrico; Negri, Pierre; Alladio, Eugenio; Salomone, Alberto; Vincenti, Marco

doi:10.1016/j.talanta.2022.123265

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…Most drugs analysed were found to be stable at 4°C for up to 40 days with extremely limited degradation. The method was applied to seven real samples, which provided quantitative results consistent with those obtained using conventional venous whole‐blood samples 34 . Similarly, cocaine and its metabolites, benzoylecgonine and cocaethylene, were found to be stable for 7 days when DBS samples were retained within the HemaPEN® device 152 .…”

Section: Applicationsmentioning

confidence: 55%

“…The method was applied to seven real samples, which provided quantitative results consistent with those obtained using conventional venous whole‐blood samples. 34 Similarly, cocaine and its metabolites, benzoylecgonine and cocaethylene, were found to be stable for 7 days when DBS samples were retained within the HemaPEN® device. 152 An LC‐MS/MS method was also established to detect 425 drugs, including several sedative hypnotics, antileptic drugs and antipsychotic drugs, in 10‐mm DBS punch samples.…”

Section: Applicationsmentioning

confidence: 95%

“… 20 Furthermore, paper‐based ionization techniques such as paper spray ionization (PSI) for MS have enabled the direct analysis of a broad spectrum of analytes in dried microsamples without the requirement of any prior sample pretreatment. 21 These sophisticated approaches have driven the implementation of microsampling in various biomedical domains; newborn and metabolic screening, 22 , 23 biomarker research, 21 , 24 , 25 pharmacokinetic (PK) and pharmacodynamic (PD) studies, 26 , 27 therapeutic drug monitoring (TDM), 28 , 29 , 30 , 31 , 32 , 33 forensic toxicology, 34 , 35 , 36 sports anti‐doping, 37 , 38 , 39 metabolomics 22 , 40 , 41 and proteomics. 24 , 42 , 43 Since 70% of medical decisions are governed by diagnostic blood tests, 44 several medical technology companies are investing in the development of innovative at‐home blood microsampling devices to make blood work more accessible and convenient.…”

Section: Introductionmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Section: Applicationsmentioning

confidence: 55%

Section: Applicationsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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show abstract

“…The The triple quadrupole tandem mass spectrometer will probably remain the preferred instrument for quantitative detection of traditional drugs of abuse, usually predetermined as target analytes. However, the retrospective investigation represents an added value for investigation of DBS samples, especially when the small amount allows only one analysis, and especially in forensic labs where there is a greater need to maximize the range of detectable compounds (Massano et al, 2022).…”

Section: Recently Proposed Methods and Applicationsmentioning

confidence: 99%

Recent advances in the detection of drugs of abuse by dried blood spots

Ververi

Vincenti

Salomone

2022

Biomedical Chromatography

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“…burden for the newborn, and at the same time, getting also the most relevant sample for determination of true remifentanil concentration in the newborn at the time of cord clamping. Recently, a method for the determination of 132 psychoactive compounds in dried blood spots has been reported, which included also remifentanil (21), but with a limit of quantitation of 5 ng mL -1 , it lacks the sensitivity needed to assess the levels possibly leading to the adverse drug effects in newborns and their mothers, which can start to occur already at 1-3 ng mL -1 .…”

Section: Introductionmentioning

confidence: 99%

Determination of remifentanil in neonatal dried blood spots by liquid chromatography-tandem mass spectrometry

Trontelj,

Rozman,

Mrhar

2024

Acta Pharmaceutica

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Remifentanil is an ultra-short-acting synthetic opioid-class analgesic which might be increasingly used “off-label” as pain management during labour. Side effects in parturients during labour, and in the infant at birth are of particular concern, especially respiratory depression which is concentration-dependent, and can occur at levels as low as 3–5 ng mL–1. The safety of such use, particularly in newborns due to remifentanil placental transfer, has not been fully demonstrated yet, partly due to the lack of a suitable non-invasive analytical method. The aim of our work was to develop a sensitive method to monitor the levels of remifentanil in neonates by a non-invasive sampling of umbi lical cord blood to support efficacy and safety trials. The presented LC-MS method is sensitive enough to reliably quantify remifentanil in just 20 µL of blood at only 0.3 ng mL–1. The dried blood spot sample preparation included solvent extraction with subsequent solid-phase extraction. The method was validated in terms of accuracy, precision, recovery, matrix effect, and stability, and was successfully applied to a small pilot study. The estimated arterial blood concentrations at the time of delivery ranged from 0.2 to 0.3, and up to 0.9 ng mL–1 in neonatal, and maternal samples, respectively.

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Development and validation of a UHPLC-HRMS-QTOF method for the detection of 132 New Psychoactive Substances and synthetic opioids, including fentanyl, in Dried Blood Spots

Cited by 13 publications

References 29 publications

Blood microsampling technologies: Innovations and applications in 2022

Blood microsampling technologies: Innovations and applications in 2022

Recent advances in the detection of drugs of abuse by dried blood spots

Determination of remifentanil in neonatal dried blood spots by liquid chromatography-tandem mass spectrometry

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