Development and Validation of a High-Performance Liquid Chromatography Method for the Determination of Histamine in Fish Samples Using Fluorescence Detection with Pre-column Derivatization

Kounnoun, Ayoub; Maadoudi, Mohamed El; Cacciola, Francesco; Mondello, Luigi; Bougtaib, Hajar; Alahlah, Naoual; Amajoud, Nadia; Baaboua, Aicha El; Louajri, Adnane

doi:10.1007/s10337-020-03909-9

Cited by 33 publications

(15 citation statements)

References 33 publications

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“…The concentration of H + in the mobile phase can affect the retention time, matrix effect, and the durability of the column, especially for pH values less than 6.0 [ 38 , 60 ]; an aqueous mobile phase with a pH of 6.9 allows a clear chromatogram with no matrix interferences to be obtained ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Cicero

Galluzzo

Cammilleri

et al. 2020

IJERPH

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We developed, validated, and confirmed with proficiency tests a fast ultra-high-performance liquid chromatography with diode array detector (UHPLC-DAD) method to determine histamine in fish and fishery products. The proposed method consists of two successive solid–liquid extractions: one with a dilute solution of perchloric acid (6%) and the second only with water. The instrumental analysis with UHPLC provides a very fast run time (only 6 min) with a retention time of approximately 4 min, a limit of quantification (LOQ) of 7.2 mg kg−1, a limit of detection (LOD) of 2.2 mg kg−1, a recovery around 100%, a relative standard deviation (RSD%) between 0.5 and 1.4, and an r2 of calibration curve equal to 0.9995. The method detected optimal values of the validation parameters and required a limited number of reagents in comparison to other methods reported in the literature. Furthermore, the method could detect histamine in a very short time compared with other methods. This method, in addition to being validated, precise, specific, and accurate, avoids wasting time, money, and resources, and limits the use of organic solvents.

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Section: Resultsmentioning

confidence: 99%

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Cicero

Galluzzo

Cammilleri

et al. 2020

IJERPH

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“…To examine the precision in terms of repeatability, accuracy (trueness, %), and recovery of the developed method, canned tuna samples were spiked at five different concentrations of histamine (6.25, 12.5, 25, 50, and 100 mg/kg), and were analyzed over six successive days with three replications per day. The precision of the assay was determined by calculating the relative standard deviation (RSD, %) for the repeated measurements, and the accuracy of the method (trueness, %) was evaluated by assessing the agreement between the measured and nominal concentrations of analyzed samples [9,10].…”

Section: Precision Accuracy and Recoverymentioning

confidence: 99%

“…As a consequence, countries such as the USA, through the Food and Drug Administration (FDA), and those of the European Union, by the Commission Regulation (EC) No. 2073/2005, have established regulatory limits for histamine levels in edible fish [8][9][10]. According to the FDA, a concentration of 50 mg/kg is an index of sample deterioration; thus, samples returning results at or above this level cannot be intended for sale or consumption.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products

et al. 2020

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Histamine poisoning is a significant public health problem. Therefore, the monitoring of histamine content in fish and fishery products is considered to be a crucial measure in the seafood industry. In the present study, a simple and rapid densitometric thin-layer chromatographic (TLC) method for histamine determination in fish samples was developed and validated. The samples were homogenized with 10% trichloroacetic acid and histamine was efficiently extracted. Then, an appropriate derivatization procedure was adopted with dansyl chloride. Once the derivatization was carried out, the samples were applied to silica gel TLC plates and developed by ascending chromatography with chloroform-triethylamine (6:4, v/v) as the mobile phase. The intensity of the histamine-dansyl derivative spots was measured by densitometry at 365 nm, and the quantitation was performed by BIO-1D image processing software. The validation of this method revealed good linearity and specificity over a concentration range from 6.25 to 100 mg/kg. Adequate precision was shown by relative standard deviations (RSD) smaller than 4.82%, accuracy ranged from −6.88% to 5.28%, and satisfactory recoveries ranging from 93% to 105% were obtained. The Limit of Detection and the Limit of Quantification were calculated at 4.4 mg/kg and 10.5 mg/kg, respectively. In addition, the effectiveness of the proposed method was assessed by the analysis of various samples, and the obtained results were confirmed with those achieved by the HPLC-UV method. Moreover, the developed method was found to be simple, cheap, and suitable for application to analyze several samples simultaneously.

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“…Common methods for BAs determination are chromatographic ones, such as thin-layer chromatography (TLC), 6,7 capillary electrophoresis (CE), [8][9][10][11] gas chromatography (GC), [12][13][14][15][16] and high-performance liquid chromatography (HPLC). [17][18][19][20][21][22][23][24][25][26][27][28][29] HPLC is the most widely used because of its high resolution, sensitivity, efficiency, and reliability. Nevertheless, a single chromatographic approach to simultaneously determine mixture of BAs without derivatization is not feasible due to the lack of adequate chromatographic or absorption/uorescence properties.…”

Section: Introductionmentioning

confidence: 99%

“…Fluorescent reagents are widely used in the pre-column or post-column derivatization in high-performance liquid chromatography coupled with uorescence detection (HPLC-FLD), which dramatically enhances the sensitivity of the detection of BAs. 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), 17,18 dansyl chloride (DNS-Cl), [19][20][21][22][23] 9-uorenylmethyl chloroformate (FMOC-Cl), 24,25 and O-phthalaldehyde (OPA) [26][27][28][29] are some popular derivatizing reagents used for the determination of BAs. AQC, DNS-Cl, and FMOC-Cl all have intrinsic uorescence and may generate uorescent by-products during the derivatization process, which complicates BAs' quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of biogenic amines in alcoholic beverages using a novel fluorogenic compound as derivatizing reagent

et al. 2021

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Development and Validation of a High-Performance Liquid Chromatography Method for the Determination of Histamine in Fish Samples Using Fluorescence Detection with Pre-column Derivatization

Cited by 33 publications

References 33 publications

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products

Determination of biogenic amines in alcoholic beverages using a novel fluorogenic compound as derivatizing reagent

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