Development and validation of a GC–FID method for quantitative analysis of oleic acid and related fatty acids

Abstract

Oleic acid is a common pharmaceutical excipient that has been widely used in various dosage forms. Gas chromatography (GC) has often been used as the quantitation method for fatty acids normally requiring a derivatization step. The aim of this study was to develop a simple, robust, and derivatization-free GC method that is suitable for routine analysis of all the major components in oleic acid USP-NF (United States Pharmacopeia-National Formulary) material. A gas chromatography–flame ionization detection (GC–FID) method was developed for direct quantitative analysis of oleic acid and related fatty acids in oleic acid USP-NF material. Fifteen fatty acids were separated using a DB-FFAP (nitroterephthalic acid modified polyethylene glycol) capillary GC column (30 m×0.32 mm i.d.) with a total run time of 20 min. The method was validated in terms of specificity, linearity, precision, accuracy, sensitivity, and robustness. The method can be routinely used for the purpose of oleic acid USP-NF material analysis.

Highly sensitive method for the quantification of trans‐linolenic acid isomers in trilinolenin of edible oils using an ionic liquid capillary column

“…The percent recovery of each ALA isomer standard added to heated trilinolenin is shown in Table . High recovery percentages of 96.2–106.5%, with CVs ranging from 0.82% to 0.97%, were attained for all ALA isomer standards and were much higher than those reported in a previous study by Zhang et al , who analyzed the 9 Z ,12 Z ,15 Z standard content and reported the recovery to be approximately 93%. Recovery values showed that the derivatization method used to determine ALA isomers in samples was appropriate.…”

COMPARISON AND VALIDATION OF a FAST METHOD FOR THE EXTRACTION AND QUANTIFICATION OF Α-Linolenic, EICOSAPENTAENOIC, AND DOCOSAHEXAENOIC FATTY ACIDS

“…In this study, results obtained by both methods (B&D and SOXTEC) are comparable with data from the aforementioned studies. Among the most widely used methods for fat extraction from fish, there is B&D (18,19) and SOXTEC is another method used to extract fat not only from fish but from different matrixes; both methods are considered accurate and precise (13). When comparing the fat extraction from fish methods, the CRM recovery percentage was taken as indicator.…”

Effect of skimmed milk and vegetable powders on shelf stability of millet‐based composite flour

“…FFA was determined according to the method described by Zhang et al . The FFA content was calculated using Eqn .…”

“…FFA and PV tests measure the primary oxidation products 19 while TBA number measures the secondary oxidation products. 20 Determination of FFA FFA was determined according to the method described by Zhang et al 21 The FFA content was calculated using Eqn (1).…”