Development and Validation of a RP-HPLC Method to Quantify Amoxicillin, Tinidazole, Esomeprazole and Lansoprazole in a Mixture

Smerikarova, Miglena; Bozhanov, Stanislav; Maslarska, Vania

doi:10.36468/pharmaceutical-sciences.611

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…The basic analytes nebivolol (Meyyanathan et al, 2008) and fluoxetine (Shukla et al, 2010) in the presence of acidic mobile phase and zwitter ionic analyte amoxicillin (Smerikarova et al, 2019) in a slightly basic environment were reported to be retained less in the nonpolar stationary phase owing to their ionization effect when determined with other analytes.…”

Section: Influence Of Mobile Phase and Phmentioning

confidence: 99%

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

V.¹,

Basuri²,

Sahini³

et al. 2022

Biomedical Chromatography

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The understanding of principles that drive the separation in reversed‐phase chromatography plays an important role in the prediction of the elution of solutes in RP‐HPLC. The separation in RP‐HPLC is based on the principle of adsorption and partition. In addition, the logP value, the pKa value of the drug and chromatographic parameters like mobile phase pH, buffer concentration, organic modifier and mobile phase additives also influence the retention and selectivity of the analyte. It was found that hydrophobic, electrostatic, hydrogen bonding and other specific interactions between the stationary phase and the solutes, along with the hydrophobicity of an analyte molecule (logP), modify the retention behaviour of the analytes. This article gives special attention to the influence of ionization and ion interaction on the separation of analytes. The drug molecules with different logP values containing protonated and deprotonated acids, bases and zwitterions are selected as examples and this article addresses various issues related to the method development, relationships between analyte retention and mobile phase pH and the pKa value of the analyte. The advances in this regard, with highlights on topics such as mechanisms of retention and various factors that influence the retention behaviour of analytes, are also updated with suitable examples.

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Section: Influence Of Mobile Phase and Phmentioning

confidence: 99%

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

V.¹,

Basuri²,

Sahini³

et al. 2022

Biomedical Chromatography

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“…Анализът беше извършен при оптималните хроматографски условия (28), без да са необходими допълнителни корекции. Беше извършено валидиране по отношение на селективност, линейност, прецизност, правилност и граници на УВОД Върху стомашната лигавица действат агресивни и протективни фактори.…”

Section: резултати и обсъжданеunclassified

“…В достъпната литература могат да бъдат открити различни методи, базирани на УВ спектрофотометрия (2,5,7,12,17,19,20,22,29), капилярна електрофореза (6,8,16,18,21) и ВЕТХ (3,9,10,13,14,23,25,27,28,30,31), за определяне на тези лекарства в различни матрици.…”

Section: резултати и обсъжданеunclassified

“…Целта на настоящата работа е да разшири приложението на по-рано разработен метод (28), като се проверят възможностите му за анализ на амоксицилин (AMOX), тинидазол (TIN) и омепразол (ОМЕ) в моделна смес.…”

Section: резултати и обсъжданеunclassified

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HPLC Determination of Amoxicillin, Tinidazole and Omeprazole in a Model Mixture

Bozhanov,

Smerikarova,

Tzvetkova

et al. 2022

vmf

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РЕЗЮМЕНастоящото изследване разглежда възможностите за едновременно определяне на амоксицилин, тинидазол и омепразол в моделна смес чрез високоефективна течна хроматография. Хроматографското разделяне беше извършено в изократичен режим на колона LiChrospher® 100 RP-18. Подвижната фаза се състои от ацетонитрил и фосфатен буфер (0.001 М, рН 7.6) в съотношение 40:60 v/v. Анализът беше извършен при скорост на потока 1,0 ml/min и дължина на вълната 280 nm. Методът беше валидиран по отношение на селективност, линейност, прецизност и точност, граница на откриване и граница на количествено определяне. Получените корелационни коефициенти R2 са по-високи от 0.999 а относителното стандартно отклонение не превишава 2%.

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“…The chemical structure of ESOPRL. The review of literature exposed the various methods used in the estimation of ESOPL such as RP-HPLC [4][5][6], RP-HPLC in presence of diclofenac sodium [7],HPLC in presence of oxytetracycline, tinidazole [8], supercritical fluid chromatography-tandem mass [9], micro-extraction-liquid chromatography-tandem mass [10], RP-HPLC, dual-wavelength and derivative spectrophotometry in presence of diclofenac [11], simultaneous electrochemical assay of ESOPL and diclofenac sodium [12], electrochemical assay using Au electrode [13], rare recent spectrophotometric assay of ESOPRL: UV-Spectroscopy [14], using 5-sulfosalicylic acid and NBS reagents [15], charge transfer reaction using chloranilic acid ( π-acceptor) [16], oxidationreduction of the ESOPL in the presence of ciprofloxacin using potassium permanganate [17], and simultaneous determination of esomeprazole magnesium trihydrate and naproxen via spectrophotometric method based on absorbance subtraction and ratio difference [18]. To our knowledge, there is no spectrophotometric method using cloud point extraction for the estimation of ESOPRL, the present developed method is based on a diazo-coupling reaction of ESOPRL with a diazotized p-nitroaniline in alkaline medium, then cloudy colored product formed by adding Triton X-114 as a surface-active area.…”

Section: Introductionmentioning

confidence: 99%

Application of the Cloud Point Extraction Method in Spectrophotometric Estimation of Esomeprazole using Diazotised p-Nitroanline and Triton X -114

Alhfidh

Othman

2021

Egypt. J. Chem.

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A simple, sensitive, and precise spectrophotometric method has been developed for the quantification of esomeprazole as pure and in its dosage form (injection). The developed method is based on the diazo-coupling reaction of esomeprazole with a diazotized p-nitroaniline to produce a yellow azo dye soluble in an aqueous medium and gave a maximum absorption at 440 nm. Then using cloud point extraction method by adding Triton X-114 to azo dye solution and heating in water-bath at 50 0 C for 25 minutes. The cloudy solution cooled in ice bath, then centrifuged for 20 minutes at 4000 rpm, separation the filtrate by decantation, and finally adding 1ml ethanol. The color of the product changed to pink color (redshift) and the pink-colored solution gave a maximum absorption at 585 nm. The optimization of the method conditions was investigated. Under the optimal conditions, Beer's law obeyed from 1 -20 µg/ml with determination coefficients (R 2 ) equal to 0.9989 and an excellent value of molar absorptivity 1.9929 x 10 4 with a limit of detection (LOD) of 0.045µg/ml and limit of quantitation (LOQ) of 0.147 μg/ml. The developed method was successfully applied to the assay of esomeprazole in injection dosage with accepted analytical results.

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Development and Validation of a RP-HPLC Method to Quantify Amoxicillin, Tinidazole, Esomeprazole and Lansoprazole in a Mixture

Cited by 9 publications

References 17 publications

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

HPLC Determination of Amoxicillin, Tinidazole and Omeprazole in a Model Mixture

Application of the Cloud Point Extraction Method in Spectrophotometric Estimation of Esomeprazole using Diazotised p-Nitroanline and Triton X -114

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