Development and validation of a UPLC method for quality control of rhubarb-based medicine: Fast simultaneous determination of five anthraquinone derivatives

Quantitative Detection of Aloin and Related Compounds Present in Herbal Products and Aloe vera Plant Extract Using HPLC Method

“…Aloe contains two major active materials: one is the aloe polysaccharides presenting in aloe filet; the other is anthraquinone derivatives existing in the leaves of different aloe plants [3]. The major constituents have multiple pharmacological actions including laxative, anti-bacterial, anti-inflammation, hemostatic, and antispasmodic [4][5][6][7][8][9]. These constituents are isolated by various extraction methods such as pressurized hot water extraction [10], ultrasonic and microwave extraction [11], supercritical CO 2 extraction [12], and pressurized liquid extraction [13].…”

Simultaneous, Simple and Rapid Determination of Five Bioactive Free Anthraquinones in Radix et Rhizoma Rhei by Quantitative1H NMR

“…10 High-performance liquid chromatography (HPLC) for determination of five free anthraquinones is frequently used for quality control of Radix et Rhizoma Rhei according to the Chinese Pharmacopoeia and related literatures. 1,9,[11][12][13][14][15][16][17][18][19] Moreover, thin-layer chromatography (TLC), 20,21 capillary electrophoresis (CE), 22,23 ultra-performance liquid chromatography (UPLC), 6,24 UPLC-mass spectrometry (MS), 3 and high speed counter current chromatography (HSCCC) 25 also have been reported for determination of the main bioactive components in Radix et Rhizoma Rhei. However, all the analytical methods above for determining and quantifying Radix et Rhizoma Rhei suffer from the intrinsic limitations of chromatography-based methods, namely, the need of standard compounds for analyte identification or quantification and the relatively long analysis times.…”

Simultaneous determination of anthraquinone derivatives in Radix et Rhizoma Rhei-based medicines by ultra-performance LC-ESCI-MS/MS multiple reaction monitoring

“…Their major active components are anthraquinone derivatives (Fig. 1), which are the basis for the quality control of Radix et Rhizoma Rhei-based medicines [7][8][9][10]. The methods commonly used for the separation and determination of all or certain of the major anthraquinone derivatives in Radix et Rhizoma Rhei are thin-layer chromatography (TLC) with UV detection [11], highspeed counter current chromatography (HSCCC) with a UV monitor [12,13], capillary electrophoresis (CE) with a photodiode array detector [14], high-performance liquid chromatography (HPLC) with a UV detector [15,16], a photodiode array detector [17], and ultra-performance liquid chromatography (UPLC) with a photodiode array detector [10].…”