Simultaneous determination of anthraquinone derivatives in Radix et Rhizoma Rhei-based medicines by ultra-performance LC-ESCI-MS/MS multiple reaction monitoring

Abstract

Summary.A new method using ultra-performance liquid chromatography (UPLC) in combination with tandem mass spectrometry and a multiple reaction monitoring mode (UPLC-MS/MS-MRM) was developed for simultaneous quantitative determination of anthraquinone derivatives in Radix et Rhizoma Rhei-based medicines. A multi-mode electrospray/chemical ionization (ESCI) and negative ion mode with [M-H] − and its fragments under collision-activated conditions were employed in MS/MS-MRM. The quantitative method was validated and applied to simultaneous determination of anthraquinone derivatives in 21 Radix et Rhizoma Rhei-based medicines. The limits of quantification were in the range of 3.90-9.09 ng mL −1 . Average recoveries were between 95.5% and 99.8% with relative standard deviations from 1.8% to 5.3%.

Determination of rhein and physcion in rhubarb by microchip capillary electrophoresis in mixed hydro‐organic solvent combined with laser‐induced fluorescence detection

“…At present, the main separation and detection methods of rhubarb active components include high-performance liquid chromatography (HPLC) [4][5][6], capillary electrophoresis (CE) [7], and tandem mass spectrometry [8,9]. Among them, the most commonly used method is HPLC, but the HPLC method consumes a lot of solvent, has a high cost, and requires a no long analysis time, ranging from 10 min [10] to tens of minutes [11,12].…”