Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin

Abstract

Considering that currently published oxaliplatin (OXPt) analytical methods require complex procedures and equipment, the objective of this paper was to present the validation of a simple, rapid, precise, and specific isocratic HPLC method for quantification of OXPt. Such method will be essential during the future development of innovative, topical drug formulations for the treatment of mucosal and skin cancers. Validated method demonstrated OXPt separation without interference from the solvents or polymers with the most relevance for developing of bioadhesive pharmaceutical drug carries (chitosan and poloxamer). Method was linear(r>0.999)over studied OXPt concentration range (0.5–15.0 µg/mL) with acceptable precision and accuracy. Limit of detection (LOD) and limit of quantification (LOQ) were 0.099 µg/mL and 0.331 µg/mL, respectively.

Versatile chromatographic method for catechin determination in development of topical formulations containing natural extracts

“…Selectivity is the ability of the method to quantify the analyte of interest, in this case the biomarker CA, in the presence of interferences (Matos et al, ). CA standard samples were contaminated with different interferences and were compared with uncontaminated samples.…”

Quality evaluation of minoxidil topical solutions obtained from magistral pharmacies

“…In vitro diffusion experiments confirmed that vesicle formulations with penetration enhancers promoted greater drug deposition on the skin [22]. Matos and co-workers (2015) described that chitosan nanoparticles increased approximately 2 fold the penetration of minoxidil sulfate into the hair follicles, compared to the control [23]. Abd and coworkers (2018) developed nanoemulsions containing eucalyptol or oleic acid, which enhanced the skin permeation of minoxidil, besides increasing the deposition of the substance in the hair follicles [24].…”

Use of natural plant extracts as a novel microbiological quality indicator in raw milk: An alternative for resazurin dye reduction method

“…The calibration curve developed by plotting the absorbance versus concentration of Hibiscus rosa-sinensis flower water extract was used to evaluate the linearity. The linearity of the proposed model was judged by examining the correlation coefficient (R 2 ) of the linear regression (Matos et al, 2015;Shabir, 2003).…”