Development and validation of a method for the quantitation of Δ9 tetrahydrocannabinol in human plasma by high performance liquid chromatography after solid-phase extraction

Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts

“…Compared to other reported GC/MS methods [14][15][16][17] for estimation of THC or its metabolites, definite advantage of our LC/MS/MS method is a gain of time. There is no need to do an elaborate sample preparation and to use various derivatization techniques for non-volatile and thermolabile compounds like in GC/MS.…”

“…Several methods are reported in the literature for the estimation of THC or its metabolites in plasma and urine by gas chromatography/mass spectrometry (GC/MS) after liquid/liquid or solid-phase extraction (SPE) and derivatization [14][15][16][17]. Major disadvantages of these methods are the elaborate sample preparation and the need to use various derivatization techniques for non-volatile and thermolabile compounds.…”

“…Major disadvantages of these methods are the elaborate sample preparation and the need to use various derivatization techniques for non-volatile and thermolabile compounds. Other reported chromatographic methods are high-performance liquid chromatography (HPLC) [14] with ultraviolet or electrochemical detection (UV, ED), and gas chromatography (GC) with electron capture, flame ionization or nitrogen-phosphorous detection (ECD, FID, NPD) [16]. Generally these methods lack either specificity or sensitivity.…”

Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass spectrometry

“…In contrast to GC, no decomposition of the cannabinoids occurs during liquid chromatography and hence the cannabinoid acid forms may be analyzed directly. Several papers report the application of LC-MS(/MS) [8][9][10][11][12][13][14]. Most of them require high sample volume (1 mL) to achieve high sensitivity, they are focused on just one compound and/or the method is not fully validated (following all criteria for chromatographic assays).…”

Determination of plasma and urine levels of Δ9-tetrahydrocannabinol and its main metabolite by liquid chromatography after solid-phase extraction

“…Most analytical methods for the determination of THC and THC-COOH in plasma and/or urine are based on liquid chromatography-mass spectrometry [24][25][26][27][28] or gas chromatography-mass spectrometry [29,30]; some liquid chromatography methods use electrochemical [31,32] or spectrophotometric [33][34][35][36][37] detection. Most of these techniques [24][25][26][27][28][29][30][31][32], however, require expensive instrumentation, which is not always available in normal analysis laboratories.…”