Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts

Grauwiler, Sandra Béatrice; Scholer, André; Drewe, Jürgen

doi:10.1016/j.jchromb.2006.12.045

Cited by 84 publications

(101 citation statements)

References 24 publications

(28 reference statements)

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“…20 A metabolite of cannabis, 11-nor-THC-9-carboxylic acid, can be detected for long periods in the urine. 21 In our study, cannabis was detected in the urine samples of 101 of 293 (34.47%) patients (Table 3). Although no difference between sex and use of cannabis was observed in our study (Table 4), a difference was determined to exist between the age groups and use of cannabis.…”

Section: Discussionmentioning

confidence: 48%

Substance Abuse Profiles of Patients Admitted to the Alcohol and Drug Addiction Research, Treatment, and Education Center in Turkey

Canbolat

Kul

Ozdemir

et al. 2017

tjps

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ÖZAmaç: Çalışmamızın amacı, AMATEM biriminde tedavi gören hastaların madde kullanım profilini onların idrar örneklerindeki madde kullanım yüzde dağılımlarına ve çoklu madde kullanımlarına göre belirlemektir. Bunun için, İstanbul Nöropsikiyatri Hastanesi AMATEM biriminde 1 Ocak 2015 -12 Aralık 2015 tarihleri arasında tedavi gören 13 ve 65 yaş aralığında erkek ve kadın 600 hastanın idrar örneklerinin analiz raporları retrospektif olarak değerlendirildi. Gereç ve Yöntemler: İdrar örnekleri Üsküdar Üniversitesi İleri Toksikoloji Analiz Laboratuvarı'na gönderildi ve orada UPLC kütle spektrometresi ile (UPLC-MS/MS) analiz edildi. AMATEM birimine başvuruda bulunan hastaların madde kullanım profillerini tanımlamak için hastaların analiz raporları üzerinden istatistiksel değerlendirme yapılmıştır. Bulgular: Altı yüz idrar örneğinin analiz raporu incelendiğinde, hastaların 293'ünde bağımlılık yapıcı madde kullanımı belirlenmiştir. İdrar örneklerinde çoğunlukla tespit edilen maddeler sırasıyla; esrar, alkol, morfin, kokain, sentetik kannabinoitler, 3,4-metilendioksimetamfetamin ve amfetamindir. Sonuç: Çalışmamızın sonuçları bütün dünyada genç nüfus tarafından esrar kullanım oranı ile benzerlik göstermektedir. Ayrıca, sentetik kannabinoitlerin çeşitliliği her yıl dünyada hızla değiştiği için çalışmamızın sonuçları ve literatüre sonuçları sentetik kannabinoit tüketimi konusunda farklılık göstermektedir. Materials and Methods:The urine samples were sent to Üsküdar University Advanced Toxicology Analysis Laboratory and were analyzed using a UPLC tandem mass spectrometer (UPLC-MS/MS). To determine the substance use profiles of the patients applying to AMATEM, statistical assessment was performed on the analysis reports of the patients. Results: When the analysis reports of the 600 urine samples were examined, 293 patients were identified to have used addictive substances. The substances most frequently detected in the urine samples were respectively: cannabis, alcohol, morphine, cocaine, synthetic cannabinoids, 3,4-Methylenedioxymethamphetamine, and amphetamine. Conclusion: The findings in our study resemble the rates of cannabis use by the young population throughout the world. Our results show differences to the literature regarding the consumption of synthetic cannabinoids because the variety of synthetic cannabinoids change rapidly around the world each year.

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Section: Discussionmentioning

confidence: 48%

Substance Abuse Profiles of Patients Admitted to the Alcohol and Drug Addiction Research, Treatment, and Education Center in Turkey

Canbolat

Kul

Ozdemir

et al. 2017

tjps

View full text Add to dashboard Cite

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“…De nombreux auteurs procèdent à une hydrolyse alcaline ou à une β-deglucuronidation avant l'extraction des composés [17][18][19][20][21]. Ceci permet de convertir les métabolites conjugués (glucuronides) en métabolites libres et de doser la totalité du composé.…”

Section: Préparationunclassified

“…Pour les concentrations dans le sérum ou le plasma, qui diffèrent de celles du sang complet, la société allemande de toxicologie et chimie forensiques (GTFCh) a également publié ses propres recommandations. Ces valeurs sont énumérées dans [11,12,18], l'urine [21], les cheveux [31], le méconium ou le cerveau (souris) [44]. Le mélange de solvant habituellement employé pour l'extraction des cannabinoïdes est un mélange hexane : acétate d'éthyle (9 : 1) [11,12,18,25,54] ou heptane : acétate d'éthyle (1 : 4) [31,43,52,53].…”

Section: Composés Matrices Et Seuils De Concentrationunclassified

“…Pour l'analyse des cannabinoïdes, les colonnes C18 sont les plus couramment utilisées, bien qu'il soit possible de sé-parer les métabolites du cannabis avec une colonne C12 [21] ou une C8 [10,16,17]. Deux méthodes ont également été mises au point avec une colonne de type phenylhexyl pour le dosage du THC-A [15] ou d'une série de plusieurs cannabinoïdes (THC, 11-OH-THC, THCCOOH et THCCOOHgluc) [58].…”

Section: Choix Des Colonnesunclassified

“…Pourtant, que ce soit en mode positif [21] ou négatif [61], l'APCI permet d'obtenir des résultats satisfaisants puisque les limites de quantification obtenues dans ce cas varient entre 1 et 5 ng/mL. Ces valeurs sont cependant lé-gèrement supérieures aux valeurs que l'on peut obtenir avec l'ionisation par électrospray.…”

Section: Ionisation Chimique à Pression Atmosphériqueunclassified

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Analyse des cannabinoïdes par spectrométrie de masse en mode tandem

Fabritius¹,

Staub²,

Giroud³

2011

Ann Toxicol Anal

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Résumé -Depuis quelques années, la spectrométrie de masse en tandem (MS/MS) ne cesse de gagner du terrain comme méthode d'analyse en toxicologie forensique, notamment pour le dosage des cannabinoïdes. Couplée à la chromatographie liquide (LC) ou gazeuse (GC), elle permet l'identification fiable et le dosage rapide du THC, de son précurseur acide, et de ses principaux métabolites, y compris les glucuronides. Au cours de ces dix dernières années, un nombre significatif de publications sont parues sur ce sujet. L'objectif de cet article est de passer en revue les analyses par spectrométrie de masse en tandem des cannabinoïdes dans diverses matrices biologiques.Mots clés : Cannabinoïdes, spectrométrie de masse en tandem, matrice biologique, chromatographie Abstract -In recent years, tandem mass spectrometry (MS/MS) is gaining ground as a reference method of analysis in clinical and forensic toxicology, especially for the determination of cannabinoids. Coupled to liquid chromatography (LC) or gas chromatography (GC), it allows the definitive identification and rapid determination of THC, its acid precursor, and its major metabolites, including the glucuronides. During the past decade, several methods of analysis of cannabinoids in different matrices have appeared on this subject. The aim of this paper is to review the analysis of cannabinoids by tandem mass spectrometry methods in various biological matrices.

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Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Battista

Sergi

Montesano

et al. 2013

Drug Testing and Analysis

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Over the last two decades, the role played by phytocannabinoids and endocannabinoids in medicine has gained increasing interest in the scientific community. Upon identification of the plant compound Δ(9)-tetrahydrocannabinol (THC) and of the endogenous substance anandamide (AEA), different methodological approaches and innovative techniques have been developed, in order to evaluate the content of these molecules in various human matrices. In this review, we discuss the analytical methods that are currently used for the identification of phytocannabinoids and endocannabinoids, and we summarize the benefits and limitations of these procedures. Moreover, we provide an overview of the main biological matrices that have been analyzed to date for qualitative detection and quantitative determination of these compounds.

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Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts

Cited by 84 publications

References 24 publications

Substance Abuse Profiles of Patients Admitted to the Alcohol and Drug Addiction Research, Treatment, and Education Center in Turkey

Substance Abuse Profiles of Patients Admitted to the Alcohol and Drug Addiction Research, Treatment, and Education Center in Turkey

Analyse des cannabinoïdes par spectrométrie de masse en mode tandem

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

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