2005
DOI: 10.1042/bst0330287
|View full text |Cite
|
Sign up to set email alerts
|

Development and use of fluorescent nanosensors for metabolite imaging in living cells

Abstract: To understand metabolic networks, fluxes and regulation, it is crucial to be able to determine the cellular and subcellular levels of metabolites. Methods such as PET and NMR imaging have provided us with the possibility of studying metabolic processes in living organisms. However, at present these technologies do not permit measuring at the subcellular level. The cameleon, a fluorescence resonance energy transfer (FRET)-based nanosensor uses the ability of the calcium-bound form of calmodulin to interact with… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

2
39
0

Year Published

2005
2005
2022
2022

Publication Types

Select...
5
2
1

Relationship

0
8

Authors

Journals

citations
Cited by 67 publications
(41 citation statements)
references
References 37 publications
2
39
0
Order By: Relevance
“…The FPs attached to the different lobes are expected to undergo a significant relative rearrangement in concert with the conformational movement. The sign of the change in FRET efficiency for each existing sensor was consistent with the change in interfluorophore distance predicted from the crystal structure (9)(10)(11)(12)35), demonstrating the importance of donor-acceptor separation in transfer efficiency. Interestingly, however, the magnitude of the FRET change of the various FLIP sensors is constant despite different absolute distance changes of the termini, suggesting that rotational averaging serves to dampen the distance effect and to change the value of the orientation factor 2 (36).…”
Section: Discussionsupporting
confidence: 52%
See 2 more Smart Citations
“…The FPs attached to the different lobes are expected to undergo a significant relative rearrangement in concert with the conformational movement. The sign of the change in FRET efficiency for each existing sensor was consistent with the change in interfluorophore distance predicted from the crystal structure (9)(10)(11)(12)35), demonstrating the importance of donor-acceptor separation in transfer efficiency. Interestingly, however, the magnitude of the FRET change of the various FLIP sensors is constant despite different absolute distance changes of the termini, suggesting that rotational averaging serves to dampen the distance effect and to change the value of the orientation factor 2 (36).…”
Section: Discussionsupporting
confidence: 52%
“…Fluorescent indicator protein (FLIP) nanosensors have thus far been developed by using type I periplasmic binding proteins (9)(10)(11)(12)19), with fluorophores attached to the N-and C-termini of the recognition element present on the two different lobes. The FPs attached to the different lobes are expected to undergo a significant relative rearrangement in concert with the conformational movement.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Nanosensors will enable compartmental analyses of metabolite levels and metabolic activity which will drive "diagnostic methodologies." Nanosensor prototypes have been expressed in Yeast and in mammalian cell cultures for determination of carbohydrate homeostasis in living cells with subcellular resolution (Fehr et al, 2005). Nanosensors can be selectively expressed under the control of tissue specific promoters.…”
Section: Discussionmentioning
confidence: 99%
“…Frommer and coworkers constructed enhanced cyan fluorescent protein-MBP-enhanced yellow fluorescent protein fusion (CFP-MBP-YFP) biosensors that they characterized in vitro and in vivo in yeast (Fehr et al, 2002, Fehr et al, 2005. The change in fluorescence ratio they observed for these sensors was around 0.1.…”
Section: Mbp-gfp Fusion Fret Sensorsmentioning
confidence: 99%